<i>16S rRNA</i>
            gene‐based metagenomic analysis of the gut microbial community associated with the DUI species
            <i>Unio crassus</i>
            (Bivalvia: Unionidae)

Mioduchowska, Monika; Zając, Katarzyna; Bartoszek, Krzysztof; Madanecki, Piotr; Kur, Jarosław; Zając, Tadeusz

doi:10.1111/jzs.12377

Cited by 14 publications

(18 citation statements)

References 51 publications

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“…The biosynthesis of secondary molecules, including enterobactins, not synthesized by mussels but found in marine mussel foot adhesive proteins, were also variable across species [52], although their potential role in freshwater mussels is unknown. These data indicate that not only is the gastrointestinal microbiome important for the diet of the host mussel species as previously suggested [11,12], but it may also be important for the physical development of the host and its activity [17].…”

Section: Discussionsupporting

confidence: 81%

“…In invertebrates, the bacterial microbiome interacts with numerous host and ecosystem functional processes, as described in sponges and corals [ 9 , 10 ]. However, in freshwater mussels, despite recent evidence suggesting that the gut microbiome plays a role in nutrient digestion [ 11 , 12 ], data on how the microbiome varies across species and environmental conditions, and the functional role of the microbiome within mussel holobionts is still largely unexplored [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

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Gut Microbiomes of Freshwater Mussels (Unionidae) Are Taxonomically and Phylogenetically Variable across Years but Remain Functionally Stable

et al. 2021

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Freshwater mussels perform essential ecosystem functions, yet we have no information on how their microbiomes fluctuate over time. In this study, we examined temporal variation in the microbiome of six mussel species (Lampsilis ornata, Obovaria unicolor, Elliptio arca, Fusconaia cerina, Cyclonaias asperata, and Tritogonia verrucosa) sampled from the same river in 2016 and 2019. We examined the taxonomic, phylogenetic, and inferred functional (from 16S rRNA sequences) facets of their microbiome diversity. Significant differences between the two years were identified in five of the six species sampled. However, not all species that exhibited a temporally variable microbiome were functionally distinct across years, indicating functional redundancy within the mussel gut microbiome. Inferred biosynthesis pathways showed temporal variation in pathways involved in degradation, while pathways involved in cellular metabolism were stable. There was no evidence for phylosymbiosis across any facet of microbiome biodiversity. These results indicate that temporal variation is an important factor in the assembly of the gut microbiomes of freshwater mussels and provides further support that the mussel gut microbiome is involved in host development and activity.

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Gut Microbiomes of Freshwater Mussels (Unionidae) Are Taxonomically and Phylogenetically Variable across Years but Remain Functionally Stable

et al. 2021

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“…Differences in microbiome composition between individuals, especially those collected from rivers or sites that differed in their physicochemistry, therefore probably reflect transient bacteria taken in from the environment during the feeding process. These transient bacteria can be abundant in Unionid mussel gut communities (Mioduchowska et al., 2020; Weingarten et al., 2019) and may contribute noise to diversity analyses, potentially accounting for differences in richness and evenness between individuals collected from different locations. How long these transient bacteria remain in the gut is unknown, as is whether they make any contribution to gut microbiome function.…”

Section: Discussionmentioning

confidence: 99%

The gut bacterial microbiome of the Threeridge mussel, Amblema plicata, varies between rivers but shows a consistent core community

2022

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1. Freshwater mussels are important for nutrient cycling and ecosystem health as they filter feed on their surrounding water. This filter feeding makes these bivalves especially sensitive to conditions in their environment. Gut microbial communities (microbiomes) have been recognised as important to both host organism and ecosystem health; however, how freshwater mussel microbiomes are organised and influenced is unclear.2. In this study, the gut bacterial microbiome of Threeridge mussel, Amblema plicata, was compared across two river basins, five rivers, and nine local sites in the south-eastern U.S.A. Mussel gut tissue was dissected, DNA extracted, and the microbiome characterised by high throughput sequencing of the V4 region of the 16S ribosomal RNA gene.3. Planctomycetes, Firmicutes, and Cyanobacteria were the most common bacterial phyla within the guts of all sampled A. plicata. However, the relative abundances of these major bacterial phyla differed between mussels sampled from different rivers and river basins, as did the relative abundance of specific bacterial operational taxonomic units (OTUs). Despite these differences, a core microbiome was identified across all mussels, with eight OTUs being consistent members of the A. plicata microbiome at all sites, the most abundant OTU identifying as a member of the family Planctomycetaceae. Geographic distance between sites was not correlated with similarity in the structure of the gut microbiome, which was more related to site physicochemistry. 4. Overall, these results suggest that while physicochemical conditions affect the composition of transient bacteria in the Threeridge mussel gut microbiome, the core microbiome is largely unaffected, and a portion of the A. plicata microbiome is retained regardless of the river system. 5. How long transient bacteria remain in the gut, and to what extent these transient microbes aid in host function is still unknown. Core microbiota have been foundto aid in multiple functions within animal hosts, and within freshwater mussels this core microbiome may aid in nutrient processing and cycling. Therefore, it is important to look at both transient and core microbes when studying the structure of freshwater invertebrate microbiomes.

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“…These differences in gut bacterial community composition among the target copepod species are considered as the results due to phylogenetic relationship. The gut bacterial communities of aquatic organisms, including invertebrates such as Bivalvia, as well as vertebrates, are strongly related to phylogeography and can be changed or maintained during the evolutionary process [ 12 , 44 ]. In the case of target copepod species in this study, Acartiidae ( A. hudsonica ) received relatively low nodal support on trees with Centropagidae ( S. tenellus ) and Pseudodiaptomidae ( P. inopinus ) using morphological data and parsimony-based phylogeny [ 45 ].…”

Section: Resultsmentioning

confidence: 99%

“…The introduction of DNA technology to microbiome analyses in both aquatic and terrestrial ecosystems has been a driving force for the study of small-sized organisms and their associated bacteria [ 11 , 12 , 13 , 14 , 15 ]. The copepod community and its associated bacterial community was also more actively analysed through the application of DNA analysis techniques; however, previous studies on copepod-associated bacteria have been primarily conducted at the laboratory scale (e.g., incubation) [ 16 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Application of Next-Generation Sequencing for the Determination of the Bacterial Community in the Gut Contents of Brackish Copepod Species (Acartia hudsonica, Sinocalanus tenellus, and Pseudodiaptomus inopinus)

Chae

Chang

et al. 2021

Animals

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The gut bacterial communities of copepods can affect metabolic processes, and consequently, their activity can be related to the release of organic substances to the environment. Hence, they are important for organic matter cycling in marine coast food webs. However, information regarding the variation in gut bacterial communities based on copepod species and environmental variations is limited. We analysed the differences in gut bacterial communities from dominant copepod species, i.e., Acartia hudsonica, Sinocalanus tenellus, and Pseudodiaptomus inopinus, in a brackish reservoir. The core bacteria among the copepod species and locations consisted of the following main operational taxonomic units (OTUs): Novosphingobium capsulatum and the family Rhodobacteraceae belonging to Alphaproteobacteria, which is abundant in seawater and freshwater aquatic ecosystems as a zooplankton-associated bacterial community. The bacterial community composition of each copepod (except the core species) showed high variability. The bacterial community diversity differed depending on the copepod species and the sites’ environmental conditions, especially salinity, e.g., compositional variations in the bacterial community of P.inopinus were high at sites with low salinity. Therefore, the gut bacterial community of each copepod species responds differently to the environment.

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16S rRNA gene‐based metagenomic analysis of the gut microbial community associated with the DUI species Unio crassus (Bivalvia: Unionidae)

Abstract: 16S rRNA gene-based metagenomic analysis of the gut microbial community associated with the DUI species Unio crassus (Bivalvia: Unionidae)A short running title: Metagenomic analysis of bivalve gut microbiome

Cited by 14 publications

References 51 publications

Gut Microbiomes of Freshwater Mussels (Unionidae) Are Taxonomically and Phylogenetically Variable across Years but Remain Functionally Stable

Gut Microbiomes of Freshwater Mussels (Unionidae) Are Taxonomically and Phylogenetically Variable across Years but Remain Functionally Stable

The gut bacterial microbiome of the Threeridge mussel, Amblema plicata, varies between rivers but shows a consistent core community

Application of Next-Generation Sequencing for the Determination of the Bacterial Community in the Gut Contents of Brackish Copepod Species (Acartia hudsonica, Sinocalanus tenellus, and Pseudodiaptomus inopinus)

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