<i>A novel facet to consider for the effects of butyrate on its target cells</i>. Focus on “The short-chain fatty acid butyrate is a substrate of breast cancer resistance protein”

Gill, Ravinder K.; Dudeja, Pradeep K.

doi:10.1152/ajpcell.00290.2011

Cited by 11 publications

(5 citation statements)

References 27 publications

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“…The role of MCT1 in the transport of butyrate into colonocytes has been well studied. MCT1 is present on the luminal membrane of the human and pig colon, and is involved in the uptake of butyrate by both proton co-transport and anion-exchange mechanisms, as has been discussed in the literature (4,27,28) . It has been demonstrated that butyrate uptake is enhanced at acidic pH and inhibited or reduced by structural analogues such as acetate, propionate, L-lactate or pyruvate (4,27) .…”

Section: Discussionmentioning

confidence: 99%

Down-regulation of monocarboxylate transporter 1 (MCT1) gene expression in the colon of piglets is linked to bacterial protein fermentation and pro-inflammatory cytokine-mediated signalling

et al. 2015

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The present study investigated the influence of bacterial metabolites on monocarboxylate transporter 1 (MCT1) expression in pigs using in vivo, ex vivo and in vitro approaches. Piglets (n 24) were fed high-protein (26 %) or low-protein (18 %) diets with or without fermentable carbohydrates. Colonic digesta samples were analysed for a broad range of bacterial metabolites. The expression of MCT1, TNF-a, interferon g (IFN-g) and IL-8 was determined in colonic tissue. The expression of MCT1 was lower and of TNF-a and IL-8 was higher with high-protein diets (P, 0·05). MCT1 expression was positively correlated with L-lactate, whereas negatively correlated with NH 3 and putrescine (P,0·05). The expression of IL-8 and TNF-a was negatively correlated with L-lactate and positively correlated with NH 3 and putrescine, whereas the expression of IFN-g was positively correlated with histamine and 4-ethylphenol (P,0·05). Subsequently, porcine colonic tissue and Caco-2 cells were incubated with Na-butyrate, NH 4 Cl or TNF-a as selected bacterial metabolites or mediators of inflammation. Colonic MCT1 expression was higher after incubation with Na-butyrate (P,0·05) and lower after incubation with NH 4 Cl or TNF-a (P,0·05). Incubation of Caco-2 cells with increasing concentrations of these metabolites confirmed the up-regulation of MCT1 expression by Na-butyrate (linear, P, 0·05) and down-regulation by TNF-a and NH 4 Cl (linear, P,0·05). The high-protein diet decreased the expression of MCT1 in the colon of pigs, which appears to be linked to NH 3 -and TNF-a-mediated signalling.

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Section: Discussionmentioning

confidence: 99%

Down-regulation of monocarboxylate transporter 1 (MCT1) gene expression in the colon of piglets is linked to bacterial protein fermentation and pro-inflammatory cytokine-mediated signalling

et al. 2015

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“…Mammalian gastrointestinal epithelia can receive and utilize butyrate as a signaling molecule to regulate proliferation, apoptosis and differentiation to adapt the growth of the bacterial community [3,4]. Butyrate also plays a role as a histone deacetylase inhibitor (HDACi) which regulates gene expressions in cells [5,6].…”

Section: Introductionmentioning

confidence: 99%

Sodium Butyrate Promotes Reassembly of Tight Junctions in Caco-2 Monolayers Involving Inhibition of MLCK/MLC2 Pathway and Phosphorylation of PKCβ2

Miao

Wang

et al. 2016

IJMS

118

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As a physiological small molecular product from the microbial fermentation of dietary fibers, butyrate plays an important role in maintaining intestinal health. Our previous works have proved that the effect of sodium butyrate (NaB) on the intestinal barrier function is mediated by activation of AMP-activated protein kinase (AMPK). However, the detailed pathway involved remains unknown. Using the calcium switch assay in the Caco-2 cell monolayer model, we found here that NaB activated AMPK mainly by increasing the calcium level, but not the ATP concentration, via promoting store-operated calcium entry (SOCE). Upon the activation of AMPK, NaB promoted the reassembly of tight junctions (TJs) based on reducing the phosphorylation of myosin II regulatory light chain (MLC2) at Ser19 and increasing phosphorylation of protein kinase C β2 (PKCβ2) at Ser660. Inhibiting (protein kinase C β) PKCβ blocked the reassembly of TJs induced by NaB in the barrier monolayer model. These results indicated that NaB could activate the calcium/calmodulin-dependent protein kinase kinase β (CaMKKβ) pathway to mediate AMPK phosphorylating, which then inhibited the phosphorylation of MLC2 and promoted the phosphorylation of PKCβ2, respectively, so that the downstream molecules of AMPK coordinately contributed to the reassembly of TJs in the Caco-2 barrier model. These results suggested a potential mechanism of butyrate for intestine homeostasis and protection.

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“…Recently, other studies have shown that several uremic toxins (such as indoxyl sulfate, p-cresyl sulfate, and hippuric acid) are generated by the gut microbiota in mice with adenine diet-induced CKD and can inhibit substrate-specific transportation mediated by ABCG2 ( Lowenstein and Nigam, 2021 ). Butyric acid produced by the gut microbiota is the substrate of ABCG2 in the colon, and the expression of ABCG2 increases alongside an increase in the butyric acid concentration ( Gill and Dudeja, 2011 ). To build on the results of the present study, it will be necessary to conduct further analyses on the effect of changes in microbial composition on UA excretion by the intestine and to identify the microbial species implicated.…”

Section: Discussionmentioning

confidence: 99%

“…The production of SCFAs in the large intestine occurs through carbohydrate fermentation ( Furuse et al, 2014 ). They include acetic acid, propionic acid, and butyric acid, which are typically produced in a ratio of about 60:25:15 and play a significant role in the colon and cecum ( Gill and Dudeja, 2011 ). Previous studies have shown that butyric acid and propionic acid can play anti-inflammatory and protective roles in the kidney.…”

Section: Discussionmentioning

confidence: 99%

Effects of polysaccharides-riched Prunus mume fruit juice concentrate on uric acid excretion and gut microbiota in mice with adenine-induced chronic kidney disease

Huang

Guo

et al. 2022

Current Research in Food Science

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A novel facet to consider for the effects of butyrate on its target cells. Focus on “The short-chain fatty acid butyrate is a substrate of breast cancer resistance protein”

Cited by 11 publications

References 27 publications

Down-regulation of monocarboxylate transporter 1 (MCT1) gene expression in the colon of piglets is linked to bacterial protein fermentation and pro-inflammatory cytokine-mediated signalling

Down-regulation of monocarboxylate transporter 1 (MCT1) gene expression in the colon of piglets is linked to bacterial protein fermentation and pro-inflammatory cytokine-mediated signalling

Sodium Butyrate Promotes Reassembly of Tight Junctions in Caco-2 Monolayers Involving Inhibition of MLCK/MLC2 Pathway and Phosphorylation of PKCβ2

Effects of polysaccharides-riched Prunus mume fruit juice concentrate on uric acid excretion and gut microbiota in mice with adenine-induced chronic kidney disease

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