Aeromonas caviae inhibits hepatic enzymes of the phosphotransfer network in experimentally infected silver catfish: Impairment on bioenergetics

Abstract

Several studies have been demonstrated that phosphotransfer network, through the adenylate kinase (AK) and pyruvate kinase (PK) activities, allows for new perspectives leading to understanding of disease conditions associated with disturbances in energy metabolism, metabolic monitoring and signalling. In this sense, the aim of this study was to evaluate whether experimental infection by Aeromonas caviae alters hepatic AK and PK activities of silver catfish Rhamdia quelen. Hepatic AK and PK activities decreased in infected animals compared to uninfected animals, as well as the hepatic adenosine triphosphate (ATP) levels. Also, a severe hepatic damage was observed in the infected animals due to the presence of dilation and congestion of vessels, degeneration of hepatocytes and loss of liver parenchyma architecture and sinusoidal structure. Therefore, we have demonstrated, for the first time, that experimental infection by A. caviae inhibits key enzymes linked to the communication between sites of ATP generation and ATP utilization. Moreover, the absence of a reciprocal compensatory mechanism between these enzymes contributes directly to hepatic damage and for a severe energetic imbalance, which may contribute to disease pathophysiology.

Antagonistic activity of a novel chitosan-selenium nanoflower against common aquaculture pathogen Aeromonas caviae

“…With a 1-2% prevalence compared to other Aeromonas species, Aeromonas caviae has attracted attention as a signi cant sh pathogen (Vega-Sánchez et al 2014). A large variety of economically signi cant sh, including Clarias gariepinus (African cat sh), Colossoma macropomum (tambaqui) Oncorhynchus mykiss (rainbow trout) Clarias batrachus (Indian walking cat sh) and Rhamdia quelen (silver cat sh) (Thomas et Baldissera et al 2018b), are susceptible to infection by this gram-negative bacterium. This bacterium has been recognized in sh as one of the most common causes of hemorrhagic septicemia in sh.…”

“…This bacterium has been recognized in sh as one of the most common causes of hemorrhagic septicemia in sh. It affects sh and is associated with hepatosplenomegaly, ulcerative skin lesions (Thomas et al 2013), and disruption of the hepatic energy metabolism (Baldissera et al 2018b). There are several reports associating signi cant sh mortality to the presence of pathogenic A. caviae (Munro et al 1993;Ringo and Vadstein 1998).…”