<i>Akkermansia muciniphila</i>counteracts the deleterious effects of dietary emulsifiers on microbiota and host metabolism

Daniel, Noëmie; Gewirtz, Andrew T.; Chassaing, Benoît

doi:10.1136/gutjnl-2021-326835

Cited by 39 publications

(21 citation statements)

References 55 publications

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“…Consistent with a previous study in which mice were administered CMC, P80, lecithin, and gum arabic, no significant difference in food intake was observed between CMC, lecithin, and control groups ( Sandall et al., 2020 ). We found that the difference in weight gain between the groups was not statistically significant as in previous studies ( Daniel, Gewirtz & Chassaing, 2023 ).…”

Section: Discussionsupporting

confidence: 55%

Inulin protects against the harmful effects of dietary emulsifiers on mice gut microbiome

Bekar,

Ozmen,

Ozkul

et al. 2024

PeerJ

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Background The prevalence of inflammatory bowel diseases is increasing, especially in developing countries, with adoption of Western-style diet. This study aimed to investigate the effects of two emulsifiers including lecithin and carboxymethyl cellulose (CMC) on the gut microbiota, intestinal inflammation and the potential of inulin as a means to protect against the harmful effects of emulsifiers. Methods In this study, male C57Bl/6 mice were divided into five groups (n:6/group) (control, CMC, lecithin, CMC+inulin, and lecithin+inulin). Lecithin and CMC were diluted in drinking water (1% w/v) and inulin was administered daily at 5 g/kg for 12 weeks. Histological examination of the ileum and colon, serum IL-10, IL-6, and fecal lipocalin-2 levels were analyzed. 16S rRNA gene V3-V4 region amplicon sequencing was performed on stool samples. Results In the CMC and lecithin groups, shortening of the villus and a decrease in goblet cells were observed in the ileum and colon, whereas inulin reversed this effect. The lipocalin level, which was 9.7 ± 3.29 ng in the CMC group, decreased to 4.1 ± 2.98 ng with the administration of inulin. Bifidobacteria and Akkermansia were lower in the CMC group than the control, while they were higher in the CMC+inulin group. In conclusion, emulsifiers affect intestinal health negatively by disrupting the epithelial integrity and altering the composition of the microbiota. Inulin is protective on their harmful effects. In addition, it was found that CMC was more detrimental to microbiota composition than lecithin.

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Section: Discussionsupporting

confidence: 55%

Inulin protects against the harmful effects of dietary emulsifiers on mice gut microbiome

Bekar,

Ozmen,

Ozkul

et al. 2024

PeerJ

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“…Prior studies have consistently identified impaired intestinal integrity, thickness of mucus layer, and immunity (low-grade inflammation) as important factors contributing to the development of metabolic syndromes related diseases [66][67][68][69] . Mucin degrading bacteria like Akkermansia muciniphila 67,70,71 and Allobaculum spp. [72][73][74] have been reported to play a key role in these processes.…”

Section: Discussionmentioning

confidence: 99%

Transfer of modified fecal viromes improve blood glucose regulation and alleviates symptoms of metabolic dysfunction-associated fatty liver disease in an obesity male mouse model

Mao

Larsen

Zachariassen

et al. 2023

Preprint

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Introduction: Recent evidence suggest a link between gut microbiome dysbiosis and metabolic syndrome, including type-2-diabetes, obesity, and non-alcoholic fatty liver disease (NAFLD). Fecal microbiota transplantation (FMT) has been explored as a way to restore a healthy gut microbiome in obese patients but poses safety concerns. The wide use of FMT is limited by safety concerns about transferring the entire fecal microbiome from one individual to another. Fecal virome transplantation (FVT) is a safer alternative that transfers bacteriophages without bacterial transfer, but still carries the risk of eukaryotic virus infection. Therefore, a safer and more effective tool for modulating gut microbiome is needed. Methods: We explored the potential of implementing three alternative FVT techniques with increased safety established in a recent study (eukaryotic viruses were either eliminated or inactivated) to ameliorate symptoms associated with a diet-induced obesity mouse model. Male mice were fed with an ad libitum high-fat diet before being euthanized (23 weeks of age) and received the different FVT treatments twice with one week of interval. Body weight was measured, oral glucose tests were performed, feces were sampled frequently, and liver, fat pads, mesenteric lymph node, and blood serum were sampled at termination of study. Results: FVT treatments had no effect on weight gain or the amount of epididymal white adipose tissue. Mice given regular untreated FVT (FVT-UnT) had a significant (p < 0.05) drop in the pathological score of their liver tissue when compared to HFD-control mice. Mice treated with a chemostat propagated fecal virome (FVT-ChP, eukaryotic viruses eliminated by dilution) improved their blood glucose regulation significantly (p < 0.05) compared to HFD-control mice. Gut microbiome analysis of both the bacterial and viral component suggested that bacteriophage-mediated modulation of the gut microbiome could be a driving factor for the observed effects. Conclusions: These results may lay the ground to develop safer bacteriophage-based therapeutic tools to restore the dysbiotic gut microbiome associated with metabolic syndrome.

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“…[ 35 ] Of note, the significantly lifted Dubosiella , was recently suggested with anti‐aging effects, [ 36 ] and Akkermansia was known for its health promoting effects. [ 37,38 ] Moreover, high relative abundance of Akkermansia and Faecalibaculum (as observed in Inulin‐L and Anti‐Inulin‐L groups after inulin supplementation stopped), was associated with hypoglycemic effect. [ 39 ] Collectively, the dominance of Dubosiella , Akkermansia , and the outgrown Faecalibaculum after restoration, pointed to the long‐lasting health promoting effect of the supplemented inulin.…”

Section: Discussionmentioning

confidence: 99%

Restoration of Intestinal Microbiota After Inulin Supplementation Halted: The Secondary Effect of Supplemented Inulin

An,

Zhou,

Zhang

et al. 2024

Molecular Nutrition Food Res

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ScopeConsumption of inulin could affect the intestinal microbiota composition. Hereby, it is aimed to investigate the intestinal microbial community restoration process when the inulin supplementation is terminated (i.e., the secondary effect).Methods and resultsThe current study investigates the response and restoration of intestinal microbiota to/after high (Inulin‐H) and low (Inulin‐L) dosage of inulin supplementation or sequential antibiotics and inulin (Anti‐Inulin‐L) supplementation, based on analysis of 16S rRNA gene sequences in C57BL/6 mice. The number of significantly changed genera in response to inulin is highest in Anti‐Inulin‐L (n = 66) group, followed by Inulin‐H (n = 51) and Inulin‐L (n = 38) group. After inulin supplementation stops, microbiota of all studied groups tend to recover to their original states, with highest percentage of inulin‐responding microbes stay significantly different at Anti‐Inulin‐L (93.94%) group, followed by Inulin‐H (74.51%) and Inulin‐L (44.12%) groups. Of note, the relative abundance of some non‐inulin‐responding taxa significantly increases during restoration.ConclusionSequential antibiotics and inulin supplementation induce greatest changes in the intestinal microbial composition, followed by high and low dosage of inulin. Additionally, the changes induce by supplemented inulin in the intestinal microbial community, provide a chance for some microbes to outcompete the other microbes during the spontaneous restoration.

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Akkermansia muciniphilacounteracts the deleterious effects of dietary emulsifiers on microbiota and host metabolism

Cited by 39 publications

References 55 publications

Inulin protects against the harmful effects of dietary emulsifiers on mice gut microbiome

Inulin protects against the harmful effects of dietary emulsifiers on mice gut microbiome

Transfer of modified fecal viromes improve blood glucose regulation and alleviates symptoms of metabolic dysfunction-associated fatty liver disease in an obesity male mouse model

Restoration of Intestinal Microbiota After Inulin Supplementation Halted: The Secondary Effect of Supplemented Inulin

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