<i>An Introduction to Dynamic Light Scattering by Macromolecules</i>

Schmitz, Kenneth S.; Phillies, George D. J.

doi:10.1063/1.2810116

Cited by 171 publications

(188 citation statements)

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“…We now note that (D 31 53 However, in multicomponent mixtures, this technique only provides some of the eigenvalues of the diffusion coefficient matrix. [54][55][56] Since cross-diffusion coefficients cannot be directly determined, each measurable eigenvalue is often assumed to be equal to a corresponding main-term diffusion coefficient.…”

Section: Resultsmentioning

confidence: 99%

Quaternary Diffusion Coefficients in a Protein−Polymer−Salt−Water System Determined by Rayleigh Interferometry

et al. 2009

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We have experimentally investigated multicomponent diffusion in a protein-polymer-salt-water quaternary system. Specifically, we have measured the nine multicomponent diffusion coefficients, D ij , for the lysozyme-poly(ethylene glycol)-NaCl-water system at pH 4.5 and 25°C using precision Rayleigh interferometry. Lysozyme is a model protein for protein-crystallization and enzymology studies. We find that the protein diffusion coefficient, D 11 , decreases as polymer concentration increases at a given salt concentration. This behavior can be quantitatively related to the corresponding increase in fluid viscosity only at low polymer concentration. However, at high polymer concentration (250 g/L), protein diffusion is enhanced compared to the corresponding viscosity prediction. We also find that a protein concentration gradient induces salt diffusion from high to low protein concentration. This effect increases in the presence of poly(ethylene glycol). Finally, we have evaluated systematic errors associated with measurements of protein diffusion coefficients by dynamic light scattering. This work overall helps characterize protein diffusion in crowded environments and may provide guidance for further theoretical developments in the field of protein crystallization and protein diffusion in such crowded systems, such as the cytoplasm of living cells.

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Section: Resultsmentioning

confidence: 99%

Quaternary Diffusion Coefficients in a Protein−Polymer−Salt−Water System Determined by Rayleigh Interferometry

et al. 2009

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“…Different theoretical models were described for simple molecule shapes (spheres, rods, random coils [24]). In case of rod-like particles (expected for VWF multimers) with diameters that are small compared with the wavelength, τ should vary as λ -3 and should go to zero as λ → ∞ [28].…”

Section: Resultsmentioning

confidence: 99%

“…The time course of the first part of the aggregation curve suggests for a diffusion limited process as the maximum aggregate size increases exponentially with time [24].…”

Section: Accepted M Manuscriptmentioning

confidence: 96%

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Kinetic study of von Willebrand factor self-aggregation induced by ristocetin

Stasio

Romitelli

Lancellotti

et al. 2009

Biophysical Chemistry

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Von Willebrand factor (VWF) is a multimeric glycoprotein present in circulating blood and in secretory granules of endothelial cells and platelets. VWF is sensitive to hydrodynamic shear stress that promotes conformational changes, rendering it able to interact with subendothelial proteins and platelets, thus promoting primary haemostasis. Likewise, the binding of the glycopeptide antibiotic ristocetin to VWF triggers hemostatically relevant conformational transitions. These changes reveal both the interaction site for platelet receptor GpIbα and the Tyr1605-Met1606 peptide bond, which is cleaved by the regulatory metalloprotease ADAMTS-13.In this study we investigated by a combined approach of light scattering spectroscopy and

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“…protein molecules in solution [14]. Thus, protein aggregation is recognizable by an increase in the hydrodynamic radius (Rh).…”

Section: Dynamic Light Scattering Analysismentioning

confidence: 99%

An approach to prevent aggregation during the purification and crystallization of wild type acyl coenzyme A: Isopenicillin N acyltransferase from Penicillium chrysogenum

Yoshida

Hensgens²,

Laan

et al. 2005

Protein Expression and Purification

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An Introduction to Dynamic Light Scattering by Macromolecules

Cited by 171 publications

References 0 publications

Quaternary Diffusion Coefficients in a Protein−Polymer−Salt−Water System Determined by Rayleigh Interferometry

Quaternary Diffusion Coefficients in a Protein−Polymer−Salt−Water System Determined by Rayleigh Interferometry

Kinetic study of von Willebrand factor self-aggregation induced by ristocetin

An approach to prevent aggregation during the purification and crystallization of wild type acyl coenzyme A: Isopenicillin N acyltransferase from Penicillium chrysogenum

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