Anguilla japonica lectin 1 delivery through adenovirus vector induces apoptotic cancer cell death through interaction with PRMT5

Abstract

The results of the present study suggest that the PRMT5-E2F-1 pathway may act as a common target for exogenous lectins including AJL1, and the cellular response to exogenous AJL1 may suggest a novel agent for cancer gene therapy. Copyright © 2016 John Wiley & Sons, Ltd.

Ulva pertusa lectin 1 delivery through adenovirus vector affects multiple signaling pathways in cancer cells

“…Our results showed that Ad-UPL1 dramatically stimulated the phosphorylation of ERK1/2 in both BEL-7404 and Huh7 cells, different from previously reported exogenous AJL1, which completely inhibited ERK1/2 phosphorylation [14]. In the meanwhile, we further showed that MEK1/2 inhibition significantly enhanced the Ad-UPL1 induced cytotoxicity.…”

“…The varied distribution of exogenous lectins may reflect varied subcellular glycosylation patterns, thus providing binding sites for different lectins. Our studies also showed that exogenous lectins including PPA, DlFBL, SpRBL, AJL1, HddSBL [11][12][13][14] as well as UPL1 induced varied intracellular signaling pathways. Taken together, varied intracellular signaling pathways affected by Fig.…”

“…The distribution of UPL1 in cell membrane, ER, and mitichondria, but not Golgi apparatus, was observed. Previously, the intracellular distribution of various exogenous lectins has been investigated in our laboratory [12][13][14]. Exogenous fucose binding lectin DlFBL was shown to be distributed in various membrane systems, including cell membrane, mitichondria, ER, and Golgi apparatus in cancer cells.…”

“…Previously, we determined that, through adenovirus-mediated gene delivery, lectins including mannose binding plant lectin Pinellia pedatisecta agglutinin (PPA) as well as marine lectins such as galectin Anguilla japonica lectin 1 (AJL1), Haliotis discus discus sialic acid binding lectin (HddSBL), Dicentrarchus labrax fucose binding lectin (DlFBL), and Strongylocentrotus purpuratus rhamnose binding lectin (SpRBL) elicited cytotoxicity to a variety of cancer cells [11][12][13][14]. Protein arginine methyltransferase 5 (PRMT5) was determined to act as a common binding target for exogenous PPA, DlFBL, SpRBL, HddSBL, as well as AJL1.…”

Marine Lectins DlFBL and HddSBL Fused with Soluble Coxsackie-Adenovirus Receptor Facilitate Adenovirus Infection in Cancer Cells BUT Have Different Effects on Cell Survival

“…For example, Maackia amurensis seed lectin [1], Concanavalin A [2], Fenneropenaeus indicus hemolymph fucose binding lectin [3], Polygonatum cyrtonema lectin [4], as well as MytiLec [5,6,7] were shown to be cytotoxic to various cancer cells through inducing apoptosis or autophagy. Through adenovirus-mediated gene delivery, the mannose binding plant lectin Pinellia pedatisecta agglutinin (PPA), as well as marine lectins, such as galectin Anguilla japonica lectin 1, Haliotis discus discus sialic acid binding lectin (HddSBL), Dicentrarchus labrax fucose binding lectin (DlFBL), and Strongylocentrotus purpuratus rhamnose binding lectin, could be exogenously expressed in various cancer cells and led to cancer cell death [8,9,10,11]. PPA delivered through a CD123 retargeted oncolytic adenovirus significantly inhibited in vivo leukemic xenograft growth, suggesting a possible anticancer lectin gene therapy strategy for cancer treatment [12].…”