<i>Annona muricata</i>modulate brain-CXCL10 expression during cerebral malaria phase

Djamiatun, Kis; Matug, Sumia M A; Prasetyo, Awal; Wijayahadi, Noor; Nugroho, Djoko

doi:10.1088/1755-1315/55/1/012034

Cited by 3 publications

(7 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most recent study shows that CXCL10 expression in the brain of severe-PbAinfected Swiss mice is modulated by ethanolic leaf extract of Annona muricata. 9 Parasitaemia, however is not affected by this extract. 9 Additionally, it has not been studied before whether artemisinin combination therapy (ACT), anti-malaria standard therapy commonly used, affect CXCL10 expressed by astrocyte during severe malaria.…”

Section: Introductionmentioning

confidence: 77%

“…9 Parasitaemia, however is not affected by this extract. 9 Additionally, it has not been studied before whether artemisinin combination therapy (ACT), anti-malaria standard therapy commonly used, affect CXCL10 expressed by astrocyte during severe malaria. Therefore it is interesting to study the influence of combination of this herb and ACT toward CXCL10 expressed by astrocytes of Swiss mice during severe malaria.…”

Section: Introductionmentioning

confidence: 77%

“…The previous study showed that the brain-CXCL10-expression in controls with severe PbA infection was significantly lower than healthy control groups. 9 This indicates that reduce the brain-CXCL10 expression associates with severe PbA infection when the highest parasitaemia levels occur.…”

Section: Discussionmentioning

confidence: 99%

“…This finding is in line with previous study using ethanolic extract of Annona muricata leaves. 9 The ethanolic extract of Annona muricata leaves in dose of 100 mg/Kg BW/day for total period of 14 days increases the brain-CXCL10-expression in severe PbA infected Swiss mice. The present study showed that the brain-CXCL10 expression of X2 and X3 group was not different than C-group.…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Effectivity Of Annona Muricata and Artemisinin Combined Therapy on Brain CXCL10 expression (Study in Swiss Mice During Severe Plasmodium Berghei ANKA Infection)

Sulayman

Djamiatun

Muniroh

2019

J. Biomed. Transl. Res.

View full text Add to dashboard Cite

Background: Malaria, caused by Plasmodium sp infection, is a major global cause of morbidity and mortality. Most experimental cerebral malaria (ECM) studies show increase number of Th1 cells and CTLs in the brain, due to increase chemokine expression, including CXCL10, a potent chemokine involved in cerebral malaria (CM). Recent studies show that CXCL10 provokes apoptosis of human brain micro-endothelial cells and in vitro neuroglia cells.Objective: To determine whether combination of Annona muricata-leaf-extracted-by-water (AME) and artemisinin-combination-therapy (ACT) reduce brain-CXCL10-expression of Swiss-mice inoculated with P. berghei ANKA (PbA). Methods: This was an experimental-study with post-test-only-control-group-design. Twenty-four Swiss-mice (PbA-inoculated) were randomly divided into 4 groups. Control group (C) was PbA inoculated only. X1, X2 and X3 groups received AME, ACT and combination of AME and ACT treatment, respectively. CXCL10 was stained with in immunohistochemistry, which then observed by light microscope in order to determine Allred-score. Kruskal-Wallis test was used to statistically analyze the differences among groups, then followed by a Mann- Whitney U test.Result: C and X1groups had severe-PbA-infection when the study was end on day-7-PbA-infection, while X2 and X3 groups entered recovery-stage. The AME-ACT-treatment-group had significantly lower of brain-CXCL10-expression than AME-group (p=0.008) and nearly significantly lower than control-group (p=0.058). Group that received ACT alone had no different value of brain-CXCL10-expression than control-group (p=0.502) and combination AME–ACT group (p=0.335).Conclusion: The combination of AME–ACT treatment decreases brain-CXCL10-expression of Swiss-mice during PbA-infection-recovery-stage, indicating the effectivity of AME–ACT combined therapy is better prevention of cerebral malaria than AME alone.

show abstract

Section: Introductionmentioning

confidence: 77%

Section: Introductionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Effectivity Of Annona Muricata and Artemisinin Combined Therapy on Brain CXCL10 expression (Study in Swiss Mice During Severe Plasmodium Berghei ANKA Infection)

Sulayman

Djamiatun

Muniroh

2019

J. Biomed. Transl. Res.

View full text Add to dashboard Cite

show abstract

“…57,58 This is in addition to the modulation of CXCL10 (a strong independent marker of CM) by A. muricata. 59 The extract of A. muricata has been reported to contain about 212 identified and isolated secondary metabolites like flavonoids, tannins, saponins, alkaloids, polyphenols, kaempferol, diterpenoids, essential oils, acetogenin compounds and megastigmanes, 60 some of which are known to possess anti-plasmodial and antimalarial effects. 61 More than 120 Acetogenins, which are the most predominant bioactive compounds, have been reported from the leaves, seeds, stems, fruit peel and pulp of A. muricata, out of which 46 have been identified from the leaves.…”

Section: Discussionmentioning

confidence: 99%

Annona muricata Linn and Khaya grandifoliola C.DC. Reduce Oxidative Stress In Vitro and Ameliorate Plasmodium berghei-Induced Parasitemia and Cytokines in BALB/c Mice

Onohuean

Alagbonsi

Usman

et al. 2021

J Evid Based Complementary Altern Med

View full text Add to dashboard Cite

Background. Annona muricata and Khaya grandifoliola are ethnomedicinally used for the treatment of malaria and have been experimentally shown to have an anti-plasmodial effect, but the mechanisms involved are not fully understood. This study investigated the effect of the ethanol extracts of their leaves on parasitemia, radical scavenging and cytokines in Plasmodium berghei ANKA-infected BALB/c mice. Methods. BALB/c mice were infected with P. berghei and treated with chloroquine, A. muricata or K. grandifoliola extract for 4 days. The percentage of parasitemia and the level of cytokine expression were determined after treatment. Trace element, phytochemical and nitric oxide (NO) scavenging activity, 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging properties assays were done to study the antioxidant effects of AN and KG in vitro. Results. P. berghei consistently increased parasitemia in BALB/c mice. The tested doses (100-, 200-, and 400 mg/kg) of A. muricata and K. grandifoliola attenuated the P. berghei-induced elevation of parasitemia and cytokines (TNF-α, IL-5, and IL-6) in vivo during the experimental period, though not as much as chloroquine. Moreover, both extracts scavenged the DPPH and NO radicals, though A. muricata had more anti-oxidant effect than K. grandifoliola in-vitro. Conclusion. The ethanol extracts of A. muricata and K. grandifoliola reduce parasitemia in P. berghei-treated mice BALB/c by scavenging free radicals and reducing cytokines, though the extracts were not as effective as chloroquine.

show abstract

Annona muricata effect on parasitemia and lymphocyte formation of act treated malaria

Dwi Fatima,

Kis Djamiatun

2023

ijamscr

View full text Add to dashboard Cite

Combination therapy is essential to safeguard existing and future antimalarial drugs, prompting exploration into adjuvant therapies for managing cerebral malaria. This study aimed to assess the effectiveness of Annona muricata leaves extracted by water (AME) as an adjuvant for Artemisinin-based combination therapy (ACT) in mice infected with Plasmodium berghei ANKA (PbA) malaria. The research employed a Post Test Only Control Group Design for the study. A few of 24 Swiss mice were subjected to various treatments, including AME, ACT, and their combination, with a focus on host survival, lymphocyte generation, and migration. Results demonstrated that mice treated with the combination of AME and ACT exhibited significantly reduced parasitemia, and lower percentages of splenic lymphoblasts compared to control groups. Specifically, the mean percentage of splenic lymphoblasts in the AME and ACT combination group was substantially lower than in the control groups and individual treatment groups. Parasitemia levels were significantly reduced in the combination group, emphasizing the synergistic effect of A. muricata and ACT. In conclusion, A. muricata demonstrated a superior impact on parasitemia, and splenic lymphoblasts in malaria mice treated with ACT, highlighting its potential as a valuable adjuvant therapy in the fight against malaria.

show abstract

Annona muricatamodulate brain-CXCL10 expression during cerebral malaria phase

Cited by 3 publications

References 49 publications

Effectivity Of Annona Muricata and Artemisinin Combined Therapy on Brain CXCL10 expression (Study in Swiss Mice During Severe Plasmodium Berghei ANKA Infection)

Effectivity Of Annona Muricata and Artemisinin Combined Therapy on Brain CXCL10 expression (Study in Swiss Mice During Severe Plasmodium Berghei ANKA Infection)

Annona muricata Linn and Khaya grandifoliola C.DC. Reduce Oxidative Stress In Vitro and Ameliorate Plasmodium berghei-Induced Parasitemia and Cytokines in BALB/c Mice

Annona muricata effect on parasitemia and lymphocyte formation of act treated malaria

Contact Info

Product

Resources

About