<i>Aphanomyces astaci</i> pathogenicity under laboratory and field conditions

Alderman, D. J.; Polglase, Jane L.; Frayling, M.

doi:10.1111/j.1365-2761.1987.tb01086.x

Cited by 71 publications

(64 citation statements)

References 6 publications

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“…Moribund individuals were removed immediately and frozen (−20°C) for further molecular analyses, and surviving crayfish were collected for molecular analyses. The temperature was adjusted to 19°C to avoid the temperature affecting the mortality rates (Alderman et al 1987, CEFAS 2000. The length of the trials was chosen to represent at least the length of the fastest growing season for crayfish during the Finnish summer.…”

Section: Infection Experiments: Environment and Experimental Designmentioning

confidence: 99%

“…In previous studies, the resistance of different crayfish species (Unestam 1969a(Unestam , 1972(Unestam , 1975 or the effects of different temperatures, water mineral content and zoospore densities to the progress of the disease (Alderman et al 1987) have been the main focus. However, possible differences in the virulence of crayfish plague isolates on noble crayfish Astacus astacus populations have not been fully evaluated.…”

Section: Introductionmentioning

confidence: 99%

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Differing virulence of Aphanomyces astaci isolates and elevated resistance of noble crayfish Astacus astacus against crayfish plague

Makkonen

Jussila²,

Kortet³

et al. 2012

Dis. Aquat. Org.

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Crayfish plague epidemics (caused by Aphanomyces astaci) have been causing population collapses among native European crayfish stocks since the late 1800s. Recent indirect and direct evidence has shown that its virulence has been variable, with native European crayfish even acting as carriers. We tested the differences in A. astaci virulence under experimental conditions using both PsI-and As-genotypes with 3 Finnish noble crayfish Astacus astacus populations. We infected crayfish with adjusted quantities of A. astaci zoospores and monitored the symptoms and mortality of the crayfish. The PsI-genotype isolate caused rapid and total mortality among the tested populations, while the As-genotype isolates expressed more variable virulence. In some cases, mortality among the As-genotype-infected crayfish did not exceed the mortality level of the control group. All of the tested noble crayfish stocks showed lower mortality towards the As-genotype of A. astaci isolated from the River Kemijoki epidemic. We conclude that there are clear differences in virulence between different A. astaci genotypes and also differences in virulence within As-genotypes. Furthermore, we observed clear signs of increased resistance in different populations of noble crayfish towards some of the tested strains belonging to the Asgenotype of A. astaci.

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Section: Infection Experiments: Environment and Experimental Designmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Differing virulence of Aphanomyces astaci isolates and elevated resistance of noble crayfish Astacus astacus against crayfish plague

Makkonen

Jussila²,

Kortet³

et al. 2012

Dis. Aquat. Org.

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“…The latter, recorded in Europe since 1860 (Vogt 1999), is responsible for numerous outbreaks of the so-called crayfish plague. From there, it had a dramatic impact on the European native crayfish populations, and its relevance has led to extensive research focused on crayfish pathology in the last 25 years (see Alderman et al 1987;Diéguez-Uribeondo, and Söderhäll 1993;Edgerton 2002a, b;Diéguez-Uribeondo et al 2007;Diéguez-Uribeondo 2009;Kozubíková et al 2010). The pathogen introduction is one of the reasons why invasive crayfish are responsible for decline or extinction of nearly one third of the world's crayfish populations (Taylor 2002).…”

Section: Introductionmentioning

confidence: 99%

A model of co-occurrence: segregation and aggregation patterns in the mycoflora of the crayfish Procambarus clarkii in Lake Trasimeno (central Italy)

et al. 2012

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“…For European and Australasian species of crayfish, the infection by the plague pathogen is fatal (ALDERMAN et al, 1987). However, crayfish species native to North America are much more resistant to the acute disease (UNESTAM, 1969;PERSSON and SÖDERHÄLL, 1983;DIÉGUEZ-URIBEONDO and SÖDERHÄLL, 1993).…”

Section: Introductionmentioning

confidence: 99%

The Crayfish Plague in the Czech Republic - Review of Recent Suspect Cases and a Pilot Detection Study

Hoffmann

Oidtmann

2006

Bull. Fr. Pêche Piscic.

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There are only very limited reports about the occurrence of the crayfish plague in Czechia. In recent years, mass mortalities of Astacus spp. with symptoms of possible crayfish plague were noticed in three streams in the country -two in Central Bohemia and one in Silesia (2004). Three dead individuals from the last outbreak were examined for the presence of the crayfish plague pathogen, Aphanomyces astaci, by observation of the presence of hyphae in their cuticle and by a PCR-based diagnostic method. In all three cases the detection was positive. Although causes of mass mortalities from two other localities lack such a direct confirmation, the indirect evidence supports the same conclusion. The main potential vector of A. astaci in Czechia is the American spiny-cheeck crayfish Orconectes limosus, widespread in large rivers of the western part of the country and in various isolated standing waters. Using the same molecular method, we investigated the presence of A. astaci in living O. limosus individuals from six localities (three running and three standing waters). The analysis indicated the presence of the pathogen in animals from five out of six investigated Orconectes populations. One of them is present in the stream where two European Astacus species had gone extinct in 1998-9. Our results suggest that the crayfish plague is still present in Czechia, and that populations of O. limosus represent a reservoir for the crayfish plague pathogen, which directly endangers populations of the native crayfish. LA PESTE DES ÉCREVISSES EN RÉPUBLIQUE TCHÈQUE -EXAMEN DES CAS SUSPECTS RÉCENTS ET UNE ÉTUDE PILOTE DE DÉTECTION RÉSUMÉIl n'y a que peu de comptes rendus sur la présence de la peste des écrevisses en République Tchèque. Ces dernières années, la mortalité d'Astacus spp. avec des symptômes de la peste des écrevisses ont été relevés dans trois cours d'eau en République tchèque -deux en Bohème centrale (1998-9) et une en Silésie (2004). On a examiné la présence d'Aphanomcyes astaci, un parasite oomycète causant cette peste, dans trois individus morts lors de la dernière épidémie, utilisant des observations directes du mycélium de l'agent pathogène dans la cuticule, ainsi qu'une méthode de diagnostic à base de PCR. À chaque occasion, les résultats furent positifs. Bien que les causes de mortalité dans deux autres localités manquent de confirmations directes, des évidences indirectes supportent la même conclusion. La peste des écrevisses est traditionnellement transmise par Orconectes limosus, une écrevisse nord américaine.

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Aphanomyces astaci pathogenicity under laboratory and field conditions

Cited by 71 publications

References 6 publications

Differing virulence of Aphanomyces astaci isolates and elevated resistance of noble crayfish Astacus astacus against crayfish plague

Differing virulence of Aphanomyces astaci isolates and elevated resistance of noble crayfish Astacus astacus against crayfish plague

A model of co-occurrence: segregation and aggregation patterns in the mycoflora of the crayfish Procambarus clarkii in Lake Trasimeno (central Italy)

The Crayfish Plague in the Czech Republic - Review of Recent Suspect Cases and a Pilot Detection Study

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