<i>Arabidopsis</i>– a model to elucidate complex stress response mechanism during cryopreservation

Stock, Johanna; Mock, Hans‐Peter; Senula, Angelika; Nagel, Manuela

doi:10.17660/actahortic.2019.1234.11

Cited by 4 publications

(2 citation statements)

References 73 publications

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“…development of forecast systems, biotechnological applications, as well as the conservation, assessment, and distribution of genetic resources of wildlife, food, and agriculture (Overmann 2015;Overmann and Smith 2017;Schüngel et al 2014;Stock et al 2018). State-of-the-art biobanks do not only provide access to high-quality biological materials but also to associated data, thereby enabling rapid response to disease outbreaks, like those of the Zika virus or COVID-19 (Peeling et al 2020), or the Panama disease threatening banana production (García-Bastidas et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Microbial occurrence in liquid nitrogen storage tanks: a challenge for cryobanking?

Bajerski

Nagel

Overmann

2021

Appl Microbiol Biotechnol

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Modern biobanks maintain valuable living materials for medical diagnostics, reproduction medicine, and conservation purposes. To guarantee high quality during long-term storage and to avoid metabolic activities, cryostorage is often conducted in the N2 vapour phase or in liquid nitrogen (LN) at temperatures below − 150 °C. One potential risk of cryostorage is microbial cross contamination in the LN storage tanks. The current review summarises data on the occurrence of microorganisms that may compromise the safety and quality of biological materials during long-term storage. We assess the potential for the microbial contamination of LN in storage tanks holding different biological materials based on the detection by culture-based and molecular approaches. The samples themselves, the LN, the human microbiome, and the surrounding environment are possible routes of contamination and can cause cross contaminations via the LN phase. In general, the results showed that LN is typically not the source of major contaminations and only a few studies provided evidence for a risk of microbial cross contamination. So far, culture-based and culture-independent techniques detected only low amounts of microbial cells, indicating that cross contamination may occur at a very low frequency. To further minimise the potential risk of microbial cross contaminations, we recommend reducing the formation of ice crystals in cryotanks that can entrap environmental microorganisms and using sealed or second sample packing. A short survey demonstrated the awareness for microbial contaminations of storage containers among different culture collections. Although most participants consider the risk of cross contaminations in LN storage tanks as low, they prevent potential contaminations by using sealed devices and − 150 °C freezers. It is concluded that the overall risk for cross contaminations in biobanks is relatively low when following standard operating procedures (SOPs). We evaluated the potential sources in detail and summarised our results in a risk assessment spreadsheet which can be used for the quality management of biobanks. Key points • Identification of potential contaminants and their sources in LN storage tanks. • Recommendations to reduce this risk of LN storage tank contamination. • Development of a risk assessment spreadsheet to support quality management.

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Section: Introductionmentioning

confidence: 99%

Microbial occurrence in liquid nitrogen storage tanks: a challenge for cryobanking?

Bajerski

Nagel

Overmann

2021

Appl Microbiol Biotechnol

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“…The long-term storage of biomaterials (biobanking) is a precondition for modern life sciences, enabling follow-up scientific investigations, medical diagnostics, biotechnological applications, and the conservation of genetic resources and diversity (Overmann 2015;Overmann and Smith 2017;Schüngel et al 2014;Stock et al 2018). To guarantee the safe storage of biological material, dedicated quality management procedures and controls need to be improved continuously (Chatterjee et al 2017;Lauterboeck et al 2016;Rittinghaus and Glasmacher 2018).…”

Section: Introductionmentioning

confidence: 99%

Factors determining microbial colonization of liquid nitrogen storage tanks used for archiving biological samples

Bajerski

Bürger

Glasmacher

et al. 2019

Appl Microbiol Biotechnol

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The availability of bioresources is a precondition for life science research, medical applications, and diagnostics, but requires a dedicated quality management to guarantee reliable and safe storage. Anecdotal reports of bacterial isolates and sample contamination indicate that organisms may persist in liquid nitrogen (LN) storage tanks. To evaluate the safety status of cryocollections, we systematically screened organisms in the LN phase and in ice layers covering inner surfaces of storage tanks maintained in different biobanking facilities. We applied a cultureindependent approach combining cell detection by epifluorescence microscopy with the amplification of groupspecific marker genes and high-throughput sequencing of bacterial ribosomal genes. In the LN phase, neither cells nor bacterial 16S rRNA gene copy numbers were detectable (detection limit, 10 2 cells per ml, 10 3 gene copies per ml). In several cases, small numbers of bacteria of up to 10 4 cells per ml and up to 10 6 gene copies per ml, as well as Mycoplasma, or fungi were detected in the ice phase formed underneath the lids or accumulated at the bottom. The bacteria most likely originated from the stored materials themselves (Elizabethingia, Janthibacterium), the technical environment (Pseudomonas, Acinetobacter, Methylobacterium), or the human microbiome (Bacteroides, Streptococcus, Staphylococcus). In single cases, bacteria, Mycoplasma, fungi, and human cells were detected in the debris at the bottom of the storage tanks. In conclusion, the limited microbial load of the ice phase and in the debris of storage tanks can be effectively avoided by minimizing ice formation and by employing hermetically sealed sample containers.

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Development of the potato cryocollection preserved in the VIR cryobank

Efremova,

Volkova,

Rybakov

et al. 2023

Trudy po prikladnoy botanike, genetike i selektsii

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Background. The article presents the results of the studies carried out within the framework of a complex program for the long-term conservation of genotyped accessions of domestic potato cultivars in the cryobank of the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR). These accessions are genetically identical to the nomenclature standards of the corresponding cultivars.Materials and methods. The research material included 24 accessions from the in vitro collection of VIR, corresponding to the nomenclature standards of Russian potato cultivars developed in different breeding centers of the Russian Federation. Cryopreservation was carried out using the droplet vitrification method, several stages of which were modified in the Department of Biotechnology of VIR.Results and discussion. The frequency of post-cryogenic regeneration for the analyzed 24 cultivars in control variants with short-term immersion of explants in liquid nitrogen varied from 20 to 47.3%, and in 9 accessions it exceeded 39% – the current level of reliable long-term preservation of samples in cryobanks. The data of the statistical analysis failed to reveal a significant effect of the genotype or the calendar dates of different experiment replications on the regeneration ability. These 24 accessions were added to the potato cryocollection stored in the VIR cryobank. An analysis of the composition and structure of VIR’s potato cryocollection, which currently includes 140 accessions, is presented.

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Arabidopsis– a model to elucidate complex stress response mechanism during cryopreservation

Cited by 4 publications

References 73 publications

Microbial occurrence in liquid nitrogen storage tanks: a challenge for cryobanking?

Microbial occurrence in liquid nitrogen storage tanks: a challenge for cryobanking?

Factors determining microbial colonization of liquid nitrogen storage tanks used for archiving biological samples

Development of the potato cryocollection preserved in the VIR cryobank

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