Arabidopsis
 C-terminal domain phosphatase-like 1 and 2 are essential Ser-5-specific C-terminal domain phosphatases

Koiwa, Hisashi; Hausmann, Stéphane; Bang, Woo Young; Ueda, Akihiro; Kondo, Naoko; Hiraguri, Akihiro; Fukuhara, Teruyuki; Bahk, Jeong Dong; Yun, Dae‐Jin; Bressan, Ray A.; Hasegawa, Paul M.; Shuman, Stewart

doi:10.1073/pnas.0403174101

Cited by 109 publications

(171 citation statements)

References 43 publications

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“…Therefore, both abnormal transcripts in rcf2-1 would encode truncated proteins with no or significantly reduced phosphatase activity. The C-terminal portion of RCF2 including the two putative dsRBMs and NLS is critical for protein-protein interactions and nuclear localization (Koiwa et al, 2004;Bang et al, 2008). Because normal RCF2 transcript was not detected in the rcf2-1 plants and because rcf2-1 is a recessive mutant, our data suggest that rcf2-1 is a loss-of-function allele of RCF2/CPL1/FRY2.…”

Section: Rcf2 Is a Positive Regulator For Freezing Tolerancementioning

confidence: 71%

“…The truncated mutant protein lacks the C-terminal 556-amino acid segment of wild-type RCF2 that contains two putative dsRBMs and an NLS (Supplemental Figure 2D). The N-terminal portion (amino acids 47 to 638) of RCF2 is essential for its phosphatase activity (Koiwa et al, 2004). Therefore, both abnormal transcripts in rcf2-1 would encode truncated proteins with no or significantly reduced phosphatase activity.…”

Section: Rcf2 Is a Positive Regulator For Freezing Tolerancementioning

confidence: 99%

“…The fry2-1 mutation causes missplicing (intron retention) of the eighth intron in the abnormal transcript detected in fry2-1 (Koiwa et al, 2004). The fry2-1 mutant produces a truncated RCF2 protein consisting of an N-terminal 676 amino acids of wild-type protein plus a C-terminal tail containing 8 extra amino acids encoded by the included eighth intron (Koiwa et al, 2004). The truncated mutant protein generated in fry2-1 lacks the C-terminal 292-amino acid segment of the wild-type RCF2 that contains the two dsRBMs and the NLS.…”

Section: Rcf2 Is a Positive Regulator For Freezing Tolerancementioning

confidence: 99%

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The Protein Phosphatase RCF2 and Its Interacting Partner NAC019 Are Critical for Heat Stress–Responsive Gene Regulation and Thermotolerance in Arabidopsis

et al. 2014

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Heat stress is a major environmental constraint for crop production worldwide. To respond to and cope with heat stress, plants synthesize heat shock proteins (HSPs), which are often molecular chaperones and are under the control of heat stress transcription factors (HSFs). Very little is known about the upstream regulators of HSFs. In a forward genetic screen for regulators of C-REPEAT BINDING FACTOR (CBF) gene expression (RCFs), we identified RCF2 and found that it is allelic to CPL1/FIERY2, which encodes a homolog of C-terminal domain phosphatase. Our results also showed that, in addition to being critical for cold stress tolerance, RCF2 is required for heat stress-responsive gene regulation and thermotolerance, because, compared with the wild type, the rcf2-1 mutant is hypersensitive to heat stress and because the reduced thermotolerance is correlated with lower expression of most of the 21 HSFs and some of the HSPs in the mutant plants. We found that RCF2 interacts with the NAC transcription factor NAC019 and that RCF2 dephosphorylates NAC019 in vivo. The nac019 mutant is more sensitive to heat stress than the wild type, and chromatin immunoprecipitation followed by quantitative PCR analysis revealed that NAC019 binds to the promoters of HSFA1b, HSFA6b, HSFA7a, and HSFC1. Overexpression of RCF2 or NAC019 in Arabidopsis thaliana increases thermotolerance. Together, our results suggest that, through dephosphorylation of NAC019, RCF2 is an integrator of high-temperature signal transduction and a mechanism for HSF and HSP activation.

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Section: Rcf2 Is a Positive Regulator For Freezing Tolerancementioning

confidence: 71%

Section: Rcf2 Is a Positive Regulator For Freezing Tolerancementioning

confidence: 99%

Section: Rcf2 Is a Positive Regulator For Freezing Tolerancementioning

confidence: 99%

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The Protein Phosphatase RCF2 and Its Interacting Partner NAC019 Are Critical for Heat Stress–Responsive Gene Regulation and Thermotolerance in Arabidopsis

et al. 2014

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“…CDKE;1, also known as HEN3, is required for the specification of stamen and carpel identities and for the proper termination of stem cells in the floral meristem (Wang and Chen, 2004). There are >20 genes encoding CTD phosphatase-like proteins (CPLs) in Arabidopsis, based on domain architecture identified in family member proteins of other eukaryotes (Koiwa et al, 2004). Genetic evidence indicates that CPL3 is a negative regulator of abiotic stress signaling (Koiwa et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Roles of Arabidopsis Cyclin-Dependent Kinase C Complexes in Cauliflower Mosaic Virus Infection, Plant Growth, and Development

et al. 2007

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The C-terminal domain (CTD) of RNA polymerase II is phosphorylated during the transcription cycle by three cyclindependent kinases (CDKs): CDK7, CDK8, and CDK9. CDK9 and its interacting cyclin T partners belong to the positive transcription elongation factor b (P-TEFb) complexes, which phosphorylate the CTD to promote transcription elongation. We report that Arabidopsis thaliana CDK9-like proteins, CDKC;1 and CDKC;2, and their interacting cyclin T partners, CYCT1;4 and CYCT1;5, play important roles in infection with Cauliflower mosaic virus (CaMV). cdkc;2 and cyct1;5 knockout mutants are highly resistant and cdkc;2 cyct1;5 double mutants are extremely resistant to CaMV. The mutants respond normally to other types of plant viruses that do not replicate by reverse transcription. Expression of a reporter gene driven by the CaMV 35S promoter is markedly reduced in the cdkc;2 and cyct1;5 mutants, indicating that the kinase complexes are important for transcription from the viral promoter. Loss of function of CDKC;1/CDKC;2 or CYCT1;4/CYCT1;5 results in complete resistance to CaMV as well as altered leaf and flower growth, trichome development, and delayed flowering. These results establish Arabidopsis CDKC kinase complexes as important host targets of CaMV for transcriptional activation of viral genes and critical regulators of plant growth and development. INTRODUCTIONThe transcription of protein-coding genes in eukaryotes is performed by RNA polymerase II (RNAP II) and forms a so-called transcription cycle that includes preinitiation, initiation, promoter clearance, elongation, and termination (Sims et al., 2004). The transcription cycle starts with the assembly of the preinitiation complex at the promoter, which includes the general transcription factors TFIID, TFIIB, TFIIE, and TFIIH as well as RNAP II. Assembly of the preinitiation complex is followed by ATPdependent melting of the double-stranded DNA (dsDNA) template and formation of an open complex between RNAP II and the DNA template. Once the open complex is established, transcription initiation occurs and RNAP II is allowed to clear the promoter and engaged to make the transition from initiation to elongation. Transcription elongation is a dynamic and highly regulated process that coordinates downstream events such as capping and splicing of primary transcripts. The final step in the cycle is transcription termination. At this stage, the mRNA is cleaved, polyadenylated, and transported to the cytoplasm for translation.The transcription cycle involving RNAP II is accompanied by another cycling event: phosphorylation of the C-terminal domain (CTD) of the largest subunit of RNAP II (Sims et al., 2004). The RNAP II CTD contains multiple repeats of the heptapeptide sequence YSPTSPS (26 such repeats in yeast, 32 in Caenorhabditis elegans, 45 in Drosophila, and 52 in mammals). RNAP II in the preinitiation complex is unphosphorylated or hypophosphorylated, whereas transcription-competent RNAP II is heavily phosphorylated on its CTD, with Ser-2 and Ser-5 as the major...

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“…The recently reported CTD phosphatase like gene in rice is involved in cell differentiation, and controls abscission layer development and seed shattering (Ji et al, 2010). Of the more than 20 genes in Arabidopsis genome that encode CTD-phosphatase like domain, only four (AtCPL1-AtCPL4) have been isolated and characterized in detail (Bang et al, 2006;Koiwa et al, 2002Koiwa et al, , 2004Ueda et al, 2008;Xiong et al, 2002). In addition to the CTD phosphatase domain, AtCPL1 and AtCPL2 harbor two and a single double-stranded RNA binding motifs (DRM) in their C-terminus, respectively (Ueda et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Mutation in OsLMS, a gene encoding a protein with two double-stranded RNA binding motifs, causes lesion mimic phenotype and early senescence in rice (Oryza sativa L.)

et al. 2012

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The rice (Oryza sativa L.) lesion mimic and senescence (lms) EMS-mutant, identified in a japonica cultivar Hitomebore, is characterized by a spontaneous lesion mimic phenotype during its vegetative growth, an accelerated senescence after flowering, and enhanced resistance to rice blast (Magnaporthe oryzae). To isolate the OsLMS gene, we crossed the lms mutant to Kasalath (indica), and used mutant F 2 plants to initially map the candidate region to about 322-kb on the long arm of chromosome 2. Illumina whole-genome re-sequencing of the mutant and aligning the reads to Hitomebore reference sequence within the candidate region delineated by linkage analysis identified a G to A nucleotide substitution. The mutation corresponded to the exon-intron splicing junction of a novel gene that encodes a carboxyl-terminal domain (CTD) phosphatase domain and two double stranded RNA binding motifs (dsRBM) containing protein. By PCR amplification, we confirmed that the mutation causes splicing error that is predicted to introduce a premature stop codon. RNA interference (RNAi) transgenic lines with suppressed expression of LMS gene exhibited the lesion mimic phenotype, confirming that the mutation identified in LMS is responsible for the mutant phenotype. OsLMS shares a moderate amino-acid similarity to the Arabidopsis FIERY2/CPL1 gene, which is known to control many plant processes such as stress response and development. Consistence with this similarity, the lms mutant shows sensitivity to cold stress at the early growth stage, suggesting that LMS is a negative regulator of stress response in rice.

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Arabidopsis C-terminal domain phosphatase-like 1 and 2 are essential Ser-5-specific C-terminal domain phosphatases

Cited by 109 publications

References 43 publications

The Protein Phosphatase RCF2 and Its Interacting Partner NAC019 Are Critical for Heat Stress–Responsive Gene Regulation and Thermotolerance in Arabidopsis

The Protein Phosphatase RCF2 and Its Interacting Partner NAC019 Are Critical for Heat Stress–Responsive Gene Regulation and Thermotolerance in Arabidopsis

Roles of Arabidopsis Cyclin-Dependent Kinase C Complexes in Cauliflower Mosaic Virus Infection, Plant Growth, and Development

Mutation in <i>OsLMS</i>, a gene encoding a protein with two double-stranded RNA binding motifs, causes lesion mimic phenotype and early senescence in rice (<i>Oryza sativa</i> L.)

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