Arabidopsis thaliana H1 histones

Abstract

We have isolated two Arubidopsis thulium cDNA clones that encode different H1 histone proteins. The H1-3 and HI-2 proteins are 274 and 273 amino acids in length, respectively. Unlike the H1 histones within a single animal species, the two plant H1 proteins share little sequence similarity outside the protein's central globular domain. Within the globular domain, a pentapeptide that is extremely well conserved in animal Hl histones, is not found in either of the plant proteins. Southern blot analysis suggests that A. thalianu has only three HI histone genes. A genomic clone encoding the HI-1 protein was isolated and the protein-coding region was found to consist of two exons separated by a 104-bp intron. The site of transcriptional initiation of the H1-I gene was mapped by primer-extension analysis and a conserved octamer motif, identical to that observed in most plant core histone genes that have been characterized to date, was found 101 nucleotides upstream of the presumed transcription-initiation site. The 3' portion of the gene encoding H1-2 was also isolated and sequenced. When the 3'-flanking regions of the two H1 genes were compared, several highly conserved sequences were observed that might be convergently transcribed relative to the histone genes.

Molecular cloning, sequence analysis and differential expression of an intron‐containing gene encoding tomato histone H1

“…In general, apicies and young tissues contain significant amounts of mRNA, but mature tissues do not. We found that the histone H1 mRNA is a polyadenylated message (data not shown) and, in this respect, it is similar to all the other reported higher plant H1 mRNAs [9,10,21,291. In contrast, the Volvox [20] and most animal cell histone H1 mRNAs are non-polyadenylated, and those which are generally fit into the category of developmental variants or replication-independent variants.…”

Deletion of the unique gene encoding a typical histone H1 has no apparent phenotype in Aspergillus nidulans

“…Second, and signi®cantly, the position of the ®rst intron of the A. nidulans gene is exactly conserved in several plant H1 genes. This intron is exactly in the same position as the single intron of the tomato H1 histone (Jayawardene and Riggs, 1994) and the ®rst intron of the genes encoding histones H1-1 of Arabidopsis thaliana (Gannt and Lenvik, 1991) and H1-I of Volvox carteri (Lindauer et al, 1993). This intron is not conserved in the other histone H1 genes found in the last two organisms.…”

“…No other gene encoding a linker histone is present in this organism. The A. nidulans coding sequence is shorter than animal (about 220 residues) and plant (240±274 residues) typical sequences (Gannt and Lenvik, 1991;Lindauer et al, 1993;Jayawardene and Riggs, 1994). The A. nidulans translated sequence lacks part of the amino terminus present in most histones of higher eukaryotes.…”

“…been cloned using anchor-dT as a primer for the ®rst strand synthesis. Poly(A)-containing transcripts have been described for histone genes from a number of organisms (Ehinger et al, 1990;Sanicola et al, 1990;Gannt and Lenvik, 1991;Ushinsky et al, 1997). The sequence of two independent cDNA clones shows a polyadenylation site at position 1528 (see Fig.…”

A wheat histone H3 promoter confers cell division‐dependent and ‐independent expression of the gus A gene in transgenic rice plants

“…In higher plants, H3 and H4 genes of wheat Tabata eta/., 1983Tabata eta/., ,1984, corn (Chaubet eta/., 1986;Gigot eta/., 1987;Phillipps eta/., 1986), rice (Wu etab, 1989b), Arabidopsis (Chaboute eta/., 1987) and alfalfa (Wu et a/., 1988) and a H I gene of Arabidopsis (Gantt and Lenvik, 1991) were isolated and their structures and sequences were analyzed. Most of the plant histone genes are dispersed in the genome except for the case of rice in which H2A, H2B and H4 genes are clustered in a 6.5 kb chromosome fragment (Thomas and PadayaW, 1983).…”