Aronia melanocarpaConcentrate Ameliorates Pro-Inflammatory Responses in HaCaT Keratinocytes and 12-O-Tetradecanoylphorbol-13-Acetate-Induced Ear Edema in Mice

Abstract

Abnormal expression of pro-inflammatory mediators such as cell adhesion molecules and cytokines has been implicated in various inflammatory skin diseases, including atopic dermatitis. In this study, we investigated the anti-inflammatory activity of Aronia melanocarpa concentrate (AC) and its action mechanisms using in vivo and in vitro skin inflammation models. Topical application of AC on mouse ears significantly suppressed 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ear edema formation, as judged by measuring ear thickness and weight, and histological analysis. Topical administration of AC also reduced the expression of pro-inflammatory cytokines such as TNF-α, IL-1β, and IL-6 in TPA-stimulated mouse ears. Pretreatment with AC suppressed TNF-α-induced ICAM-I expression and subsequent monocyte adhesiveness in human keratinocyte cell line HaCaT. In addition, AC significantly decreased intracellular reactive oxygen species (ROS) generation as well as mitogen-activated protein kinase (MAPK) activation in TNF-α-stimulated HaCaT cells. AC and its constituent cyanidin 3-glucoside also attenuated TNF-α-induced IKK activation, IκB degradation, p65 phosphorylation/nuclear translocation, and p65 DNA binding activity in HaCaT cells. Overall, our results indicate that AC exerts anti-inflammatory activities by inhibiting expression of pro-inflammatory mediators in vitro and in vivo possibly through suppression of ROS-MAPK-NF-κB signaling pathways. Therefore, AC may be developed as a therapeutic agent to treat various inflammatory skin diseases.

Topical Application of Aronia melanocarpa Extract Rich in Chlorogenic Acid and Rutin Reduces UVB-Induced Skin Damage via Attenuating Collagen Disruption in Mice

“…In addition, excessive accumulation of ROS increases MMP activity, resulting in destruction of extracellular matrix (ECM) via collagen degradation [ 36 ]. Additionally, it has been reported that topical administration of AME ameliorates pro-inflammatory response via suppression of ROS-MAPK-NF-kB signaling pathway in a mouse model of tetradecanoylphorbol-13-acetate (TPA)-induced ear edema model [ 37 ].…”

“…However, there was a limitation of the qualitative analysis of the ingredients contained in the OJE; it was poorly acknowledged which components of the OJE contributed to the protective effect against UVB-induced skin damage. In a few studies, AME showed an improvement in inflammation in a mouse model of ear edema [ 37 ], but the active ingredient of the extract was not reported. Our present results present not only the protective effect of the AME against UVB-induced skin damage but also present major ingredients of the AME containing phenolic compounds including chlorogenic acid and rutin.…”

Leaves of Raphanus sativus L. Shows Anti-Inflammatory Activity in LPS-Stimulated Macrophages via Suppression of COX-2 and iNOS Expression.

“…Many pharmacologically active compounds isolated from natural products were developed as medicines (5). In particular, food-based compounds with anti-inflammatory activities have gained widespread attention owing to their safety (6–8). …”

“…Inflammation is mediated by several factors and cellular signaling molecules that are released from macrophages (6,16). When the macrophages are stimulated by endotoxins, such as lipopolysaccharides (LPS), they release various proinflammatory mediators and cytokines, such as nitric oxide (NO), prostaglandin E 2 (PGE 2 ), tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, and IL-6 (16).…”

Inhibition of Lipid Peroxidation of Kiwicha (Amaranthus caudatus) Hydrolyzed Protein Using Zebrafish Larvae and Embryos

“…Other authors reported the use of DCFH-DA to inhibit ROS in cells. Goh et al (2016), described ROS inhibition in keratinocyte cell lines, HaCaT, and monocytic THP-1 cell lines treated with concentrate obtained from Alonia species [ 60 ]. Jensen et al (2015) reported ROS inhibition in polymorphonuclear cells in an extract obtained from blue-green algae [ 61 ].…”