Aspergillus SteA (Sterile12‐like) is a homeodomain‐C2/H2‐Zn+2 finger transcription factor required for sexual reproduction

Vallim, Marcelo A.; Miller, Karen Y.; Miller, Bruce L.

doi:10.1046/j.1365-2958.2000.01874.x

Cited by 157 publications

(165 citation statements)

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“…When environmental and internal conditions are met, GprA and GprB are expressed, and sensitization of these GPCRs would exert activation of the FadA-SfaDTGpgA heterotrimer and the subsequent signaling cascade for cleistothecia development. This branch may be composed of SteC (MAPKKK) and SteA (a Ste12 homolog) in A. nidulans (Vallim et al 2000;Wei et al 2003). Further genetic and biochemical studies must be carried out to understand the molecular mechanisms underlying signal transduction from GPCRs to G-proteins to downstream effectors that selectively determine cellular responses.…”

Section: G203rmentioning

confidence: 99%

Multiple Roles of a Heterotrimeric G-Protein γ-Subunit in Governing Growth and Development of Aspergillus nidulans

Seo¹,

Han²,

2005

Genetics

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Vegetative growth signaling in the filamentous fungus Aspergillus nidulans is primarily mediated by the heterotrimeric G-protein composed of FadA (Ga), SfaD (Gb), and a presumed Gg. Analysis of the A. nidulans genome identified a single gene named gpgA encoding a putative Gg-subunit. The predicted GpgA protein consists of 90 amino acids showing 72% similarity with yeast Ste18p. Deletion (D) of gpgA resulted in restricted vegetative growth and lowered asexual sporulation. Moreover, similar to the DsfaD mutant, the DgpgA mutant was unable to produce sexual fruiting bodies (cleistothecia) in self-fertilization and was severely impaired with cleistothecial development in outcross, indicating that both SfaD and GpgA are required for fruiting body formation. Developmental and morphological defects caused by deletion of flbA encoding an RGS protein negatively controlling FadA-mediated vegetative growth signaling were suppressed by DgpgA, indicating that GpgA functions in FadA-SfaD-mediated vegetative growth signaling. However, deletion of gpgA could not bypass the need for the early developmental activator FluG in asexual sporulation, suggesting that GpgA functions in a separate signaling pathway. We propose that GpgA is the only A. nidulans Gg-subunit and is required for normal vegetative growth as well as proper asexual and sexual developmental progression.

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Section: G203rmentioning

confidence: 99%

Multiple Roles of a Heterotrimeric G-Protein γ-Subunit in Governing Growth and Development of Aspergillus nidulans

Seo¹,

Han²,

2005

Genetics

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“…This process needs molecular communication between mating partners, followed by finely organized morphogenetic changes, regulated by a number of genes that are affected by either intrinsic and/or environmental stimuli, like age of the culture, temperature, nutrient status or light. Early studies (Dyer et al 1992) suggested that sexual morphogenesis in fungi is under polygenic control and several genes involved in sexual communication have already been characterized in detail (Shen et al 1999;Vallim et al 2000; Pö ggeler and Kü ck 2001; Kim et al 2002;Turina et al 2003;Kim and Borkovich 2004). Nevertheless the molecular mechanisms of this series of events are still not fully understood (Coppin et al 1997;Kronstadt and Staben 1997;Shiu and Glass 2000;Trail et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Tagging target genes of the MAT1-2-1 transcription factor in Fusarium verticillioides (Gibberella fujikuroi MP-A)

Keszthelyi

Jeney

Kerényi

et al. 2006

Antonie van Leeuwenhoek

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Mating type in filamentous ascomycetes is controlled by idiomorphic alleles, named MAT1-1 and MAT1-2, which contain 1-3 genes. Of these genes MAT1-1-1 and MAT1-2-1 encode putative transcription factors and are thus considered to be the major regulators of sexual communication and mating. Fungi with no known sexual stage may also have fully functional mating type genes and therefore it was plausible to hypothesize that the MAT products may also regulate other types of genes not involved directly in the mating process. To identify putative target genes of these transcription factors in Fusarium verticillioides, DMAT1-2-1 knock out mutants were produced and transcript profiles of mutant and wild type were compared by means of differential cDNA hybridization. Clones, either up-or down-regulated in the DMAT1-2-1 mutant were sequenced and a total of 248 sequences were blasted against the NCBI database as well as the Gibberella zeae and Gibberella moniliformis genomes. Fifty-five percent of the clones were down-regulated in the mutant, indicating that the MAT1-2-1 product positively affected these tagged sequences. On the other hand, 45% were found to be up-regulated in the mutant, suggesting that the MAT1-2-1 product also exerted a negative regulatory function on this set of genes. Sequences involved in protein synthesis and metabolism occurred more frequently among the clones up-regulated in the mutant, whereas genes belonging to cell signalling and communication were especially frequently tagged among the sequences down-regulated in the mutant.

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“…After Fus3 is activated, it enters the nucleus and phosphorylates Ste12, a mating-type-specific transcription factor, as well as two regulators of Ste12, Dig1 and Dig2 (Blackwell et al 2007). Activated Ste12 regulates the expression of many genes involved in the mating process, both indirectly and directly by binding pheromone response elements in target promoters (Zeitlinger et al 2003).Ste12-like proteins have also been studied in filamentous fungi, where they play essential roles in development and pathogenicity (Alspaugh et al 1998;Vallim et al 2000;Borneman et al 2001;Park et al 2002;Tsuji et al 2003;Li et al 2005;Nolting and Poggeler 2006;Ren et al 2006;Tollot et al 2009;Rispail and Di Pietro 2010;Wong Sak Hoi and Dumas 2010). Fus3-like proteins in filamentous fungi could directly or indirectly phosphorylate Ste12-like proteins, but this has not yet been shown in any system.…”

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confidence: 99%

“…All Ste12-like proteins contain a divergent homeodomain (STE) near their N terminus, which is involved in DNA binding (Errede and Ammerer 1989). Ste12-like proteins in filamentous fungi also contain two C-terminal C2H2-Zn 2+ motifs (Vallim et al 2000;Chang et al 2004) ( Figure 1A) that are absent in S. cerevisiae and other related ascomycete yeast species. In the plant pathogen Magnaporthe grisea, both the STE domain and the C2H2-Zn 2+ motifs of the STE12 homolog, mst12, are essential for the development of appressorial penetration pegs (Park et al 2004), while in the human basidiomycete pathogen Cryptococcus neoformans, the STE domain and the C2H2-Zn 2+ motifs were important for function, although mutagenesis of each domain resulted in strains with different phenotypes (Chang et al 2004).…”

mentioning

confidence: 99%

Early Colony Establishment in Neurospora crassa Requires a MAP Kinase Regulatory Network

Leeder¹,

Jonkers²,

Li³

et al. 2013

Genetics

129

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Vegetative fusion is essential for the development of an interconnected colony in many filamentous fungi. In the ascomycete fungus Neurospora crassa, vegetative fusion occurs between germinated conidia (germlings) via specialized structures termed "conidial anastomosis tubes" (CATs) and between hyphae within a mature colony. In N. crassa, both CAT and hyphal fusion are under the regulation of a conserved MAP kinase cascade (NRC1, MEK2, and MAK2). Here we show that the predicted downstream target of the MAK2 kinase pathway, a Ste12-like transcription factor known as PP1, regulates elements required for CAT and hyphal fusion. The PP1 regulatory network was revealed by expression profiling of wild type and the Dpp-1 mutant during conidial germination and colony establishment. To identify targets required for cell fusion more specifically, expression-profiling differences were assessed via inhibition of MAK2 kinase activity during chemotropic interactions and cell fusion. These approaches led to the identification of new targets of the cell fusion pathway that, when mutated, showed alterations in chemotropic signaling and cell fusion. In particular, conidial germlings carrying a deletion of NCU04732 (Dham-11) failed to show chemotropic interactions and cell fusion. However, signaling (as shown by oscillation of MAK2 and SO to CAT tips), chemotropism, and cell fusion were restored in Dham-11 germlings when matched with wild-type partner germlings. These data reveal novel insights into the complex process of self-signaling, germling fusion, and colony establishment in filamentous fungi. CELL fusion between genetically identical cells is important in development (for example, myoblast fusion during muscle formation) and occurs in many multicellular organisms from simple ascomycete fungi to mammals (Chen et al. 2007;Aguilar et al. 2013). Cell fusion between genetically identical cells can be mediated by cells that have differentiated, but in some cases, also between cells in an identical developmental state, for example, cell fusion between germinating asexual spores (conidia) of filamentous fungi (Pandey et al. 2004;Roca et al. 2005a;Read et al. 2010). In filamentous fungi, these fusions are integral to the formation of an interconnected hyphal network, which mediates genetic mixing and the sharing of resources (Simonin et al. 2012;Roper et al. 2013). How this process is initiated and maintained and what proteins are involved are still mostly unknown.In filamentous ascomycete fungi, a conserved MAP kinase pathway that is involved in pheromone response and mating in Saccharomyces cerevisiae (Ste11, Ste7, and Fus3) (Bardwell 2005) is required for cell fusion and heterokaryon formation during vegetative growth (Hou et al. 2002;Wei et al. 2003;Pandey et al. 2004;Fu et al. 2011;Jun et al. 2011;Dettmann et al. 2012). This conserved pathway also plays a role in sexual development and secondary metabolism and is required for the virulence of both plant and animal fungal pathogens (Roman et al. 2007;Rispail and Di Piet...

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Aspergillus SteA (Sterile12‐like) is a homeodomain‐C₂/H₂‐Zn⁺² finger transcription factor required for sexual reproduction

Cited by 157 publications

References 83 publications

Multiple Roles of a Heterotrimeric G-Protein γ-Subunit in Governing Growth and Development of Aspergillus nidulans

Multiple Roles of a Heterotrimeric G-Protein γ-Subunit in Governing Growth and Development of Aspergillus nidulans

Tagging target genes of the MAT1-2-1 transcription factor in Fusarium verticillioides (Gibberella fujikuroi MP-A)

Early Colony Establishment in Neurospora crassa Requires a MAP Kinase Regulatory Network

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