<i>AtNFXL1</i>, an Arabidopsis homologue of the human transcription factor NF‐X1, functions as a negative regulator of the trichothecene phytotoxin‐induced defense response

Asano, Taku; Masuda, Daisuke; Yasuda, Michiko; Nakashita, Hideo; Kudo, Toshiaki; Kimura, Makoto; Yamaguchi, Kazuo; Nishiuchi, Takumi

doi:10.1111/j.1365-313x.2007.03353.x

Cited by 48 publications

(34 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As for WRKY3, its expression is strongest in seeds, with an increase during final seed maturation (Figure 7). In Arabidopsis, NFXL1 has been implicated in the trichothecene phytotoxin-induced response as well as in the general defense response (Asano et al, 2008). In addition, plants overexpressing NFXL1 display a higher survival when subjected to abiotic stresses such as salt, drought, or high light intensity (Lisso et al, 2006).…”

Section: Link Between Seed Longevity and Genetic Pathways Regulating mentioning

confidence: 99%

Inference of Longevity-Related Genes from a Robust Coexpression Network of Seed Maturation Identifies Regulators Linking Seed Storability to Biotic Defense-Related Pathways

Righetti¹,

Vu²,

Pelletier³

et al. 2015

Plant Cell

121

191

View full text Add to dashboard Cite

Seed longevity, the maintenance of viability during storage, is a crucial factor for preservation of genetic resources and ensuring proper seedling establishment and high crop yield. We used a systems biology approach to identify key genes regulating the acquisition of longevity during seed maturation of Medicago truncatula. Using 104 transcriptomes from seed developmental time courses obtained in five growth environments, we generated a robust, stable coexpression network (MatNet), thereby capturing the conserved backbone of maturation. Using a trait-based gene significance measure, a coexpression module related to the acquisition of longevity was inferred from MatNet. Comparative analysis of the maturation processes in M. truncatula and Arabidopsis thaliana seeds and mining Arabidopsis interaction databases revealed conserved connectivity for 87% of longevity module nodes between both species. Arabidopsis mutant screening for longevity and maturation phenotypes demonstrated high predictive power of the longevity cross-species network. Overrepresentation analysis of the network nodes indicated biological functions related to defense, light, and auxin. Characterization of defense-related wrky3 and nf-x1-like1 (nfxl1) transcription factor mutants demonstrated that these genes regulate some of the network nodes and exhibit impaired acquisition of longevity during maturation. These data suggest that seed longevity evolved by co-opting existing genetic pathways regulating the activation of defense against pathogens.

show abstract

Section: Link Between Seed Longevity and Genetic Pathways Regulating mentioning

confidence: 99%

Inference of Longevity-Related Genes from a Robust Coexpression Network of Seed Maturation Identifies Regulators Linking Seed Storability to Biotic Defense-Related Pathways

Righetti¹,

Vu²,

Pelletier³

et al. 2015

Plant Cell

121

191

View full text Add to dashboard Cite

show abstract

“…The AtNFXL1 promoter activity was apparently induced by T-2 toxin and DAS in Arabidopsis plants. 7 The fold increase of promoter activities of T-2 toxin-treated plants was higher than that of DAS-treated plants. In contrast, DON only weakly induced promoter activity in AtNFXL1 promoter::GUS plants.…”

mentioning

confidence: 88%

“…Correspondingly, an atnfxl1 mutant exhibited a severe growth defect on MS medium containing 0.1 μM T-2 toxin compared to wild type. 7 Microarray analysis suggested that the atnfxl1 mutant could not appropriately repress the defense response induced by T-2 toxin, resulting in severe growth defects in T-2 toxin-treated Arabidopsis seedlings. 7 As presented in Figure 1, growth defect by DAS was also enhanced in the atnfxl1 mutant.…”

mentioning

confidence: 99%

“…7 Microarray analysis suggested that the atnfxl1 mutant could not appropriately repress the defense response induced by T-2 toxin, resulting in severe growth defects in T-2 toxin-treated Arabidopsis seedlings. 7 As presented in Figure 1, growth defect by DAS was also enhanced in the atnfxl1 mutant. Hypersensitivity phenotype of the T-2 toxin-treated atnfxl1 mutant is more severe than that of the DAS-treated mutant (Fig.…”

mentioning

confidence: 99%

“…In contrast, DON only weakly induced promoter activity in AtNFXL1 promoter::GUS plants. 7 Therefore, AtNFXL1 gene exhibited type A trichothecenedependent expression pattern. Correspondingly, an atnfxl1 mutant exhibited a severe growth defect on MS medium containing 0.1 μM T-2 toxin compared to wild type.…”

mentioning

confidence: 99%

See 2 more Smart Citations

TheAtNFXL1gene functions as a signaling component of the type A trichothecene-dependent response

Asano

Yasuda

Nakashita

et al. 2008

Plant Signaling & Behavior

Self Cite

View full text Add to dashboard Cite

profiles, suggesting that these chemotypes play a role in the virulence of individual Fusarium strains. 2 Trichothecenes are classifiable into four groups by their characteristic functional groups. Type A [T-2 toxin and DAS] and type B [nivalenol (NIV) and DON] trichothecenes, which are distinguishable by the absence or presence of a carbonyl group at the C8 position, have frequently contaminated in cereal crops and processed grains. Trichothecenes inhibit peptidyl transferase activity in eukaryotic cells by binding to the 60S ribosomal subunit. 3 Therefore, trichothecenes are considered to inhibit the defense response of host plants. However, we showed that type A trichothecenes, such as T-2 toxin and DAS, induce an elicitor-like signaling pathway and cell death in Arabidopsis thaliana at a concentration of 1 μM. 4 It is likely that type A trichothecene-induced cell death contributes directly to virulence of necrotrophic fungi. In contrast, 5-10 μM DON apparently inhibits protein translation in Arabidopsis cells, but fails to activate the elicitor-like signaling pathway. 4 These results suggest that Fusarium species use DON as a non-defense-inducing translational inhibitor during disease spread in host plants. The role of trichothecene in virulence is likely to differ greatly among its molecular species.Furthermore, we performed a comparative analysis of the phytotoxic action of representative trichothecenes when Arabidopsis grew on media containing these compounds. 4 Both DON and DAS) preferentially inhibited root elongation. Preferential inhibition of root elongation was also observed in plants treated with another phytotoxin, coronatine. 5 In addition, T-2 toxin-treated seedlings exhibited dwarfism with aberrant morphological changes (e.g., petiole shortening, curled dark-green leaves and reduced cell size). These results imply that the phytotoxic action of trichothecenes differed among their molecular species. Seedlings treated with another translational inhibitor, cycloheximide (CHX), did not display these features. Although the DAS structure closely resembles that of T-2 toxin, DAS and T-2 toxin are distinguished by the presence and absence, respectively, of an isovaleryl group at the C8 position. In addition, phytotoxic effects of HT-2 toxin (type A with the isovaleryl group) are comparable to those of T-2 toxin, whereas T-2 tetraol (type A without the isovaleryl group) did not have these effects (data not shown). It might be that the isovaleryl group at the C8 position affects the mode of action of trichothecenes in host plants, causing morphological change of Arabidopsis shoots. Phytotoxins represent a diverse group of secondary fungal metabolites, which vary widely in their chemistry and toxicology. Trichothecene phytotoxins are produced by necrotrophic phytopathogens such as Fusarium species. 1 Trichothecene-producing Fusarium species have strain-specific trichothecene metabolite

show abstract

A physiological perspective of late maturation processes and establishment of seed quality in Medicago truncatula seeds

Verdier

Leprince

Buitink

2019

The Model Legume Medicago Truncatula

View full text Add to dashboard Cite

AtNFXL1, an Arabidopsis homologue of the human transcription factor NF‐X1, functions as a negative regulator of the trichothecene phytotoxin‐induced defense response

Cited by 48 publications

References 39 publications

Inference of Longevity-Related Genes from a Robust Coexpression Network of Seed Maturation Identifies Regulators Linking Seed Storability to Biotic Defense-Related Pathways

Inference of Longevity-Related Genes from a Robust Coexpression Network of Seed Maturation Identifies Regulators Linking Seed Storability to Biotic Defense-Related Pathways

TheAtNFXL1gene functions as a signaling component of the type A trichothecene-dependent response

A physiological perspective of late maturation processes and establishment of seed quality in Medicago truncatula seeds

Contact Info

Product

Resources

About