<i>Babesia vogeli</i>
            in dogs in Chile

Cataldo, Sophia Di; Claudia, Ulloa-Contreras; Cevidanes, Aitor; Hernández, Claudia; Millán, Javier

doi:10.1111/tbed.13609

Cited by 14 publications

(18 citation statements)

References 26 publications

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“…DNA was not detected during this survey. Babesia vogeli was recently found in Chile [36], is surprising we had not found it despite been present the competent vector R. sanguineus. Absent of Hepatozoon spp.…”

Section: Discussionmentioning

confidence: 49%

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Canine vector-borne pathogens in rural dogs in Chile: molecular survey and co-infection patterns

Cevidanes¹,

Cataldo²,

Martín³

et al. 2020

Preprint

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Background: Canine vector-borne pathogens (CVBP) comprise a relevant and globally distributed group of disease agents. The aim of this study is to determine de co-occurrence of the most relevant CVBP of veterinary and zoonotic interest, in free-ranging, owned, rural dogs of central Chile, and to evaluate risk factors and potential non-apparent hematological alterations associated to pathogen co-infection by two or more agents.Methods: Nine groups of canine vector-borne pathogens (CVBP) were molecularly investigated in 111 free-ranging, owned rural dogs in the Metropolitan Region of Chile. Results: At least one pathogen was detected in 75% of the dogs. The most prevalent agent was Anaplasma platys (36%), followed by Candidatus Mycoplasma haematoparvum (CMhp; 31%), Mycoplasma haemocanis (Mhc; 28%), Trypanosoma cruzi (17%), Leishmania spp. (4.5%) and Acanthocheilonema reconditum (1%). DNA of Ehrlichia spp., Rickettsia spp., Bartonella spp., Piroplasmida and Hepatozoon spp. was not detected. Thirty-eight dogs (34%) were coinfected, either by two (n=20), three (n=7), or four agents (n=1). The most common co-infection pattern was CMhp – Mhc (n=14). CMhp was involved in 71%, Mhc in 58%, and A. platys in 50% of the co-infections. Prevalence of A. platys was higher in juvenile than in adult dogs, whereas the opposite was found for CMhp and Mhc. Adult dogs had five times more probabilities of being coinfected than young animals. Dogs positive for A. platys were infested by a larger number of Rhipicephalus sanguineus sensu lato ticks than uninfected individuals. At clinical evaluation, most of the animals were considered healthy, with only eight dogs (7%) presenting pale mucous membranes. Co-infected animals showed higher white blood cell count, segmented neutrophil count and GGT levels than non-co-infected dogs. Conclusions: Widespread occurrence of and co-infection by CVBP was found in rural free-ranging dogs of central Chile. This study represents the first report of Leishmania spp. in Chile. Clinically healthy but infected dogs as those studied here may act as reservoirs of CVBP, potentially contributing to the spread of these pathogens to other tick-exposed dogs as well as human beings or wild carnivores.

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Section: Discussionmentioning

confidence: 49%

“…Canine babesiosis in South America is caused by the intraerythrocytic protozoan parasites Babesia vogeli and Babesia gibsoni [34,35]. Recently, B. vogeli has been reported in Chile [36]. Dog population in Chile was estimated at 4.059.200 individuals [37], and owned free-roaming dogs (i.e.…”

Section: Introductionmentioning

confidence: 99%

Canine vector-borne pathogens in rural dogs in Chile: molecular survey and co-infection patterns

Cevidanes¹,

Cataldo²,

Martín³

et al. 2020

Preprint

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“…All the sequences analyzed in this study were consistent with Babesia vogeli. In general, this has been the most frequently detected species in dogs in Brazil (Passos et al, 2005;Duarte et al, 2011;O'Dwyer et al, 2009;Spolidorio et al, 2011;Lemos et al, 2012, Moraes et al, 2015, Sousa et al, 2017Barbosa et al 2020, Castro et al, 2020 and also others countries as China, Sudan, Spain, Venezuela, Chile, and USA (Li et al, 2020;Oyamada et al, 2005;Criado-Fornelio et al, 2007;Di Cataldo et al, 2020;Rey-Valeiron et al, 2007;Passos et al, 2005).…”

Section: Discussionmentioning

confidence: 98%

Molecular evaluation of piroplasms and hematological changes in canine blood stored in a clinical laboratory in Niterói, Rio de Janeiro

Santos

Corrêa

Lobão

et al. 2020

Rev. Bras. Parasitol. Vet.

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Piroplasm species were analyzed by molecular tools in total 31 blood samples from positive dogs, previously checked by stained slides, stored until DNA extraction between 2016 to 2018 in the laboratory Clinical Analyzes in Niterói, Rio de Janeiro. The piroplasms were identified by PCR, targeting the 18S rRNA gene and sequencing. From the total number of samples only 24 (77.4%) were positive and show adequate nucleotide sequences for interpretation with identity between 93%-100% with Babesia vogeli in compared to the sequences isolated of infected dogs from other states in Brazil deposited on GenBank. Most of dogs infected with B. vogeli had anemia (62.5%) and thrombocytopenia (95.8%). The findings of this study are compatible with previous reports in the literature and highlight B. vogeli as the most incriminated species in canine piroplasmosis in Brazil, and thrombocytopenia the hematological alteration most frequently identified in this infection. It is important to note that this is the first study involving the molecular characterization of piroplasms in the metropolitan region of Rio de Janeiro, based on PCR followed by sequencing.

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“…The molecular analyses of the present study only identified B. vogeli. In fact, B. vogeli is the piroplasmid species that has been most detected in dogs in different regions of Brazil [16,18,25,28,[30][31][32][35][36][37], and in other countries [37][38][39][40][41][42]. However, in addition to this species, the piroplasmid R. vitalii has also been detected in dogs in the southern region of Brazil and in municipalities in the mountain region of the state of Rio de Janeiro, by means of molecular tools [13,14,16].…”

Section: Discussionmentioning

confidence: 99%

Microscopic Detection, Hematological Evaluation and Molecular Characterization of Piroplasms from Naturally Infected Dogs in Rio de Janeiro, Brazil

et al. 2021

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Purpose This study aimed to analyze the frequency of piroplasmids in the blood of dogs in Rio de Janeiro, compare the performance of microscopic techniques, assess the risk factors associated with infections and also molecularly and morphologically characterize the piroplasmids identified. Methods In all, 407 blood samples were collected from dogs between 2018 and 2019. These were subjected to microscopic parasitological techniques for thin and thick smears, stained with Giemsa and using a rapid staining kit. The slides were read under an optical microscope and the protozoa were characterized morphometrically. In addition, the blood samples were subjected to molecular characterization for diagnosing piroplasmid species using primers that amplified the gene 18S rRNA. Results Piroplasmids were detected in 38 (9.3%) samples. Of these, 33 samples presented nucleotide sequences compatible with Babesia vogeli. Most of the positive samples were young, male, defined breeds dogs that had been attended in clinics in São Gonçalo city. Thrombocytopenia and leukopenia were the hematological alterations more observed in positive samples, but positive samples without alterations were also detected. The sex was the only variable that showed statistical differences. Males dogs being more often infected than females (p < 0.05). The microscope slides mostly showed piriform and oval merozoites measuring greater than 2.5 µm in length, which were compatible with B. vogeli. However, smaller forms were also identified, thus demonstrating the polymorphic nature of this parasite. Conclusion Babesia vogeli was detected in blood samples from dogs in the metropolitan cities of Rio de Janeiro by molecular techniques in different parasite morphotypes.

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Babesia vogeli in dogs in Chile

Cited by 14 publications

References 26 publications

Canine vector-borne pathogens in rural dogs in Chile: molecular survey and co-infection patterns

Canine vector-borne pathogens in rural dogs in Chile: molecular survey and co-infection patterns

Molecular evaluation of piroplasms and hematological changes in canine blood stored in a clinical laboratory in Niterói, Rio de Janeiro

Microscopic Detection, Hematological Evaluation and Molecular Characterization of Piroplasms from Naturally Infected Dogs in Rio de Janeiro, Brazil

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