<i>Bartonella henselae</i>and<i>B. koehlerae</i>DNA in Birds

Mascarelli, Patricia E.; McQuillan, Maggie; Harms, Craig A.; Harms, Ronald V.; Breitschwerdt, Edward B.

doi:10.3201/eid2003.130563

Cited by 22 publications

(18 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bartonella species have been validated (Buffet, Kosoy, & Vayssier-Taussat, 2013;Kosoy & Goodrich, 2019), from which 17 show zoonotic potential (Chomel et al, 2009;Maggi et al, 2011;Mascarelli, McQuillan, Harms, Harms, & Breitschwerdt, 2014).…”

Section: Introductionmentioning

confidence: 99%

Multi‐locus sequencing reveals a novel Bartonella in mammals from the Superorder Xenarthra

Calchi

Vultão

Alves

et al. 2020

Transbound Emerg Dis

View full text Add to dashboard Cite

The superorder Xenarthra consists of sloths, anteaters and armadillos, mammals originated from South America and currently distributed from the south of North America to the south of South America. The present study aimed to investigate the occurrence and genetic diversity of Bartonella spp. in blood and spleen samples from free‐living Xenarthra mammals in the states of São Paulo (SP), Mato Grosso do Sul (MS), Rondônia (RO) and Pará (PA). Based on a quantitative real‐time PCR (qPCR) assay, a Bartonella spp. nuoG gene fragment was detected in 1.51% (5/330) of the samples: 4 six‐banded armadillos (Euphractus sexcinctus) sampled in the MS and 1 southern tamandua (Tamandua tetradactyla) sampled in the PA. Eight sequences (5 ftsZ, 2 gltA and 1 rpoB) were obtained in the conventional PCR assays. In both phylogenetic analyses based on Bayesian and distance (SplitsTree) methods, the obtained ftsZ, gltA and rpoB sequences were positioned in a distinct clade, but related to B. washoensis. The analysis of SplitsTree and genotype networks based on B. washoensis sequences from several hosts from various localities of the world showed that the sequences of the present study were allocated in a group separated from the other sequences, indicating that they probably originated from median vectors and large numbers of mutational events. Additionally, the analyses performed by BLAST showed low percentages of identities of the sequences obtained in the present study when compared to those previously deposited in GenBank. Therefore, we propose a new Candidatus to Bartonella occurring in Xenarthra in Brazil. The present study was the first to report the occurrence of Bartonella sp. in mammals of the superorder Xenarthra in the world, and it was the first to describe a new Candidatus related to B. washoensis in Brazil.

show abstract

Section: Introductionmentioning

confidence: 99%

Multi‐locus sequencing reveals a novel Bartonella in mammals from the Superorder Xenarthra

Calchi

Vultão

Alves

et al. 2020

Transbound Emerg Dis

View full text Add to dashboard Cite

show abstract

“…A recent molecular study has been carried out on ticks collected from migratory birds in Italy, which found that they are often carriers of ticks, in particular Hyalomma marginatum, infected by Rickettsia sp, B. burgdorferi s.l., C. burnetii and Babesia microti [5]. However, data about the detection of tick-borne bacteria in birds are scant [4,6,7,8], and in particular, there is no data available on the prevalence of these pathogens among birds in Italy.…”

Section: Introductionmentioning

confidence: 99%

Serological evidence of exposure to zoonotic tick-borne bacteria in pheasants (Phasianus colchicus)

Ebani¹,

Bertelloni²,

Mani³

2017

Ann Agric Environ Med.

View full text Add to dashboard Cite

Introduction and objective. Previous studies on tick-borne pathogens in the avian population have focused mainly on the detection of the agents in ticks collected from birds, but data about the presence of tick-borne bacteria in these animals are scant. The aim of the presented study was to verify the exposure to some zoonotic tick-borne bacteria, in particular, Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato, Coxiella burnetii, and the Rickettsia species of the Spotted Fever Group (SFG), in pheasants (Phasianus colchicus) living in a central Italy area, characterized by conditions favourable for the diffusion of the ticks. Materials and method. Blood serum samples from 276 farm-reared pheasants were examined by indirect immunofluorescence antibody test to detect antibodies against the 4 reported pathogens. Results. A total of 124 (44.92%) birds resulted as seropositive: in particular 3 (1.08%) to C. burnetii, 31 (11.23%) to A. phagocytophilum, 46 (16.67%) to B. burgdorferi s.l., 49 (17.75%) to SFG Rickettsia spp. antigens. Three pheasants resulted positive both to A. phagocytophilum and B. burgdorferi s.l. antigens, whereas 2 -both to B. burgdorferi s.l. and SFG Rickettsia spp. antigens. The antibody titers varied from 1:40-1:320. Conclusions. The obtained results show that the pheasants had developed antibodies to the investigated tick-borne agents. For this reason, they seem to be involved in the epidemiology of the studied tick-borne bacteria.

show abstract

“…These five libraries were processed and sequenced separately 59 from the other samples, but by using identical procedures. 60 Library preparation and sequencing. We amplified and sequenced the V4 61 hypervariable region of 16S rRNA gene.…”

mentioning

confidence: 99%

“…Bartonella are gram-negative Bacteria that are typically transmitted by blood 240 sucking arthropods, and are infectious in mammalian hosts [56][57][58]. There are also 241 reports on Bartonella incidence in birds [59,60], and it is conceivable that the Bacteria 242 originate from the birds, rather than from the mites. That would suggest that the host 243 range for Bartonella spp.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Microbial composition of enigmatic bird parasites:WolbachiaandSpiroplasmaare the most important bacterial associates of quill mites (Acari: Syringophilidae)

Glowska

Filutowska

Dabert

et al. 2018

Preprint

View full text Add to dashboard Cite

Background The microbiome is an integral component of many animal species, potentially affecting behaviour, physiology, and other biological properties. Despite this importance, bacterial communities remain vastly understudied in many groups of invertebrates, including mites. Quill mites (Acariformes: Syringophilidae) are a poorly known group of permanent bird ectoparasites that occupy quills of feathers and feed on bird subcutaneous tissue and fluids. Most of the known species have strongly female biased sex ratio and it was hypothesized that this is caused by endosymbiotic bacteria.Their peculiar lifestyle further makes them potential vectors for bird diseases.Previously, Anaplasma phagocytophilum and a high diversity of Wolbachia strains were detected in quill mites via targeted PCR screens. Here, we use an unbiased 16S amplicon sequencing approach to determine other Bacteria that potentially impact quill mite biology. ResultsWe performed 16S V4 amplicon sequencing of 126 quill mite individuals from eleven species parasitizing twelve bird species (four families) of passeriform birds. In addition to Wolbachia, we found Spiroplasma as potential symbiont of quill mites.Interestingly, consistently high Spiroplasma titres were only found in individuals of two mite species associated with finches of the genus Carduelis, suggesting a history of horizontal transfers of Spiroplasma via the bird host. Furthermore, there was evidence for Spiroplasma negatively affecting Wolbachia titres. We found no evidence for the previously reported Anaplasma in quill mites, but detected the potential pathogensBrucella and Bartonella at low abundances. Other amplicon sequence variants (ASVs) could be assigned to a diverse number of bacterial taxa, including several that were 1/17 previously isolated from bird skin. We observed a relatively uniform distribution of these ASVs across mite taxa and bird hosts, i.e, there was a lack of host-specificity for most detected ASVs. Further, many frequently found ASVs were assigned to taxa that show a very broad distribution with no strong prior evidence for symbiotic association with animals. We interpret these findings as evidence for a scarcity or lack of resident microbial associates (other than inherited symbionts) in quill mites, or for abundances of these taxa below our detection threshold.Hi-Q TM View OT2 Kit with regard to manufacturer's instructions. Sequencing of the 66 templated ISPs was conducted on the Ion 318 TM Chip with the use of Ion PGM TM 67 Hi-Q TM View Sequencing Kit and Ion PGM system (Ion Torrent, Thermo Fisher

show abstract

Bartonella henselaeandB. koehleraeDNA in Birds

Cited by 22 publications

References 10 publications

Multi‐locus sequencing reveals a novel Bartonella in mammals from the Superorder Xenarthra

Multi‐locus sequencing reveals a novel Bartonella in mammals from the Superorder Xenarthra

Serological evidence of exposure to zoonotic tick-borne bacteria in pheasants (Phasianus colchicus)

Microbial composition of enigmatic bird parasites:WolbachiaandSpiroplasmaare the most important bacterial associates of quill mites (Acari: Syringophilidae)

Contact Info

Product

Resources

About