Bauhinia variegataLeaf Extracts Exhibit Considerable Antibacterial, Antioxidant, and Anticancer Activities

Abstract

The present study reports the phytochemical profiling, antimicrobial, antioxidant, and anticancer activities of Bauhinia variegata leaf extracts. The reducing sugar, anthraquinone, and saponins were observed in polar extracts, while terpenoids and alkaloids were present in nonpolar and ethanol extracts. Total flavonoid contents in various extracts were found in the range of 11–222.67 mg QE/g. In disc diffusion assays, petroleum ether and chloroform fractions exhibited considerable inhibition against Klebsiella pneumoniae. Several other extracts also showed antibacterial activity against pathogenic strains of E. coli, Proteus spp. and Pseudomonas spp. Minimum bactericidal concentration (MBC) values of potential extracts were found between 3.5 and 28.40 mg/mL. The lowest MBC (3.5 mg/mL) was recorded for ethanol extract against Pseudomonas spp. The antioxidant activity of the extracts was compared with standard antioxidants. Dose dependent response was observed in reducing power of extracts. Polar extracts demonstrated appreciable metal ion chelating activity at lower concentrations (10–40 μg/mL). Many extracts showed significant antioxidant response in beta carotene bleaching assay. AQ fraction of B. variegata showed pronounced cytotoxic effect against DU-145, HOP-62, IGR-OV-1, MCF-7, and THP-1 human cancer cell lines with 90–99% cell growth inhibitory activity. Ethyl acetate fraction also produced considerable cytotoxicity against MCF-7 and THP-1 cell lines. The study demonstrates notable antibacterial, antioxidant, and anticancer activities in B. variegata leaf extracts.

Bioactive characterization of Persea americana Mill. by-products: A rich source of inherent antioxidants

“…As it was mention before, Epicatechin was the most abundant compound in both by-products, peels are also majoritarian in different sort of B-type epicatechin dimer, trimer, tetramers and flavonol glycosides. On one hand Hollman et al (1999) reports higher antioxidant activity on aglycones compared with glycosides, while Mishra et al (2013) point out the difference between dimers, trimers, tetramers, heptamers and hexamers specificity on their properties as an antioxidants. These and other interaction could explain the differences obtained in the antioxidant activity between P. americana by-products, but in order to obtain better comprehension on this phenomena, more analysis have to be performed.…”

Iron Chelating Activity of Gossypitrin Isolated from the Petals of Talipariti elatum Sw. (Fryxell) Malvaceae

“…Because of their capacity to chelate metal ions (iron, copper, etc. ), flavonoids also inhibit free radical generation Mishra et al, 2013b). The flavonoid heterocycle contributes to antioxidant activity by permitting conjugation between the aromatic rings and the presence of a free 3-OH.…”

“…Because of their capacity to chelate metal ions (iron, copper, etc. ), flavonoids also inhibit free radical generation Mishra et al, 2013b). Quercetin in particular is known for its iron-chelating and iron-stabilizing properties.…”

A review of the use of pteridophytes for treating human ailments

“…Bio-compounds containing antioxidant potential have the capacity to inhibit carcinogenesis (Wang et al, 2011;Firdaus et al, 2013). In earlier studies, apigenin, quercetin, and luteolin were shown to inhibit cancer cell proliferation (Mishra et al, 2013a(Mishra et al, , 2013bFotsis et al, 1997). Moreover, phenolics have been observed to postpone or inhibit the progression of various diseases by preventing peroxidation of lipid membranes .…”