<i>Blastomyces dermatitidis</i>
            : Production of the Sexual Stage

McDonough, E. S.; Lewis, Ann L.

doi:10.1126/science.156.3774.528

Cited by 62 publications

(26 citation statements)

References 11 publications

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“…4; see Table S3 in the supplemental material). These results were also obtained previously by laboratory crossings with their relatives H. capsulatum and B. dermatitidis (3,4). Coiled hyphae were observed in the paired T1F1 ϫ T15LN1 and T9B1 ϫ T15LN1 P. brasiliensis iso- Idiomorphic distribution of 98 isolates revealing that 56% harbored MAT1-1, 31% harbored MAT1-2, and 13% harbored both MAT1-1 and MAT1-2.…”

Section: Expression Of Sex-related Genes In Yeast and Mycelial Cells supporting

confidence: 73%

“…These results were obtained in mating experiments using V8, SEA, and OTA media, with samples being monitored for 6 months. The resemblance of the coiled hypha aggregations to immature ascocarps echoes previous observations in H. capsulatum, B. dermatitidis, Emmonsia spp., A. fumigatus, A. nidulans, and Trichophyton mentagrophytes, whose sexual cycles are known (3,4,(59)(60)(61)(62). Coiled hyphae were detected after 2 months of mating assays of isolates of opposite mating types for both species of Paracoccidioides; in fact, this is the first step of ascogenesis, as also reported for H. capsulatum and Aspergillus spp.…”

Section: Discussionsupporting

confidence: 49%

“…Also, a sexual cycle may occur. Mating via pheromone exchange in a heterothallic bipolar system in the presence of mycelial cells of different mating types has been described for Ajellomyces dermatitidis (3), Ajellomyces capsulatus (4,5), Ajellomyces parva, and Ajellomyces crescens (6).The initial step of mating in species of the Ajellomycetaceae family is triggered by hyphal constriction of cells of different mating types, coiling with one another several times to produce young ascocarps. Once hyphal aggregations become tighter, the cleistothecium is formed, with evanescence of the ascus, which harbors meiosporic spores (4).…”

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confidence: 99%

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Molecular and Morphological Data Support the Existence of a Sexual Cycle in Species of the Genus Paracoccidioides

et al. 2013

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The genus Paracoccidioides includes the thermodimorphic species Paracoccidioides brasiliensis and P. lutzii, both of which are etiologic agents of paracoccidioidomycosis, a systemic mycosis that affects humans in Latin America. Despite the common occurrence of a sexual stage among closely related fungi, this has not been observed with Paracoccidioides species, which have thus been considered asexual. Molecular evolutionary studies revealed recombination events within isolated populations of the genus Paracoccidioides, suggesting the possible existence of a sexual cycle. Comparative genomic analysis of all dimorphic fungi and Saccharomyces cerevisiae demonstrated the presence of conserved genes involved in sexual reproduction, including those encoding mating regulators such as MAT, pheromone receptors, pheromone-processing enzymes, and mating signaling regulators. The expression of sex-related genes in the yeast and mycelial phases of both Paracoccidioides species was also detected by realtime PCR, with nearly all of these genes being expressed preferentially in the filamentous form of the pathogens. In addition, the expression of sex-related genes was responsive to the putative presence of pheromone in the supernatants obtained from previous cocultures of strains of two different mating types. In vitro crossing of isolates of different mating types, discriminated by phylogenetic analysis of the ␣-box (MAT1-1) and the high-mobility-group (HMG) domain (MAT1-2), led to the identification of the formation of young ascocarps with constricted coiled hyphae related to the initial stage of mating. These genomic and morphological analyses strongly support the existence of a sexual cycle in species of the genus Paracoccidioides.

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Section: Expression Of Sex-related Genes In Yeast and Mycelial Cells supporting

confidence: 73%

Section: Discussionsupporting

confidence: 49%

mentioning

confidence: 99%

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Molecular and Morphological Data Support the Existence of a Sexual Cycle in Species of the Genus Paracoccidioides

et al. 2013

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“…Sexual reproduction has been described for H. capsulatum (142,146), B. dermatitidis (194), and most dermatophyte species (307). While successful mating has not yet been observed for P. marneffei, C. immitis, or C. posadasii, genes related to sexual reproduction have been identified in the genomes of these dimorphic fungal species (70,189,311).…”

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confidence: 99%

The Evolution of Sex: a Perspective from the Fungal Kingdom

Lee

et al. 2010

Microbiol Mol Biol Rev

323

344

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SUMMARY Sex is shrouded in mystery. Not only does it preferentially occur in the dark for both fungi and many animals, but evolutionary biologists continue to debate its benefits given costs in light of its pervasive nature. Experimental studies of the benefits and costs of sexual reproduction with fungi as model systems have begun to provide evidence that the balance between sexual and asexual reproduction shifts in response to selective pressures. Given their unique evolutionary history as opisthokonts, along with metazoans, fungi serve as exceptional models for the evolution of sex and sex-determining regions of the genome (the mating type locus) and for transitions that commonly occur between outcrossing/self-sterile and inbreeding/self-fertile modes of reproduction. We review here the state of the understanding of sex and its evolution in the fungal kingdom and also areas where the field has contributed and will continue to contribute to illuminating general principles and paradigms of sexual reproduction.

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“…The analyses by Matute et al [23] also provided evidences supporting recombination in nature of at least one of the P. brasiliensis species, thus indicating the presence of sexual reproduction. This is reinforced by the presence of teleomorphs in close relatives of P. brasiliensis, such as B. dermatitidis (Ajellomyces dermatitidis) [68] and H. capsulatum (Ajellomyces capsulatum) [69].…”

Section: Impact Of Pbgp43 Polymorphism At the Protein Levelmentioning

confidence: 99%