<i>Blautia</i>Abundance and Mucosal Barrier Injury: A Complex Play of Cause and Effect

Mooij, Charlotte E. M. de; Groningen, Lenneke F.J. van; Molendijk, Eva B D; Wardill, Hannah R.; Velden, Walter J.F.M. van der; Blijlevens, Nicole M. A.

doi:10.1093/cid/ciac887

Cited by 7 publications

(7 citation statements)

References 8 publications

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“…In addition, the abnormal enrichment of Blautia, a member of the Firmicutes phylum, in the CK group can lead to intestinal mucosal damage and disrupt the intestinal barrier [33]. These alterations in the microbiota are one of the reasons why the control group's intestinal structure is damaged and has not been able to repair itself.…”

Section: Discussionmentioning

confidence: 99%

Hyaluronan with Different Molecular Weights Can Affect the Gut Microbiota and Pathogenetic Progression of Post-Intensive Care Syndrome Mice in Different Ways

Jiang

Zhu

et al. 2023

IJMS

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Post-intensive care syndrome (PICS) poses a serious threat to the health of intensive care unit (ICU) survivors, and effective treatment options are currently lacking. With increasing survival rates of ICU patients worldwide, there is a rising interest in developing methods to alleviate PICS symptoms. This study aimed to explore the potential of using Hyaluronan (HA) with different molecular weights as potential drugs for treating PICS in mice. Cecal ligation and puncture (CLP) were used to establish a PICS mice model, and high molecular weight HA (HMW-HA) or oligo-HA were used as therapeutic agents. Pathological and physiological changes of PICS mice in each group were monitored. 16S rRNA sequencing was performed to dissect gut microbiota discrepancies. The results showed that both molecular weights of HA could increase the survival rate of PICS mice at the experimental endpoint. Specifically, 1600 kDa-HA can alleviate PICS in a short time. In contrast, 3 kDa-HA treatment decreased PICS model survivability in the early stages of the experiment. Further, via 16S rRNA sequence analysis, we observed the changes in the gut microbiota in PICS mice, thereby impairing intestinal structure and increasing inflammation. Additionally, both types of HA can reverse this change. Moreover, compared to 1600 kDa-HA, 3 kDa-HA can significantly elevate the proportion of probiotics and reduce the abundance of pathogenic bacteria (Desulfovibrionaceae and Enterobacteriaceae). In conclusion, HA holds the advantage of being a potential therapeutic drug for PICS, but different molecular weights can lead to varying effects. Moreover, 1600 kDa-HA showed promise as a protective agent in PICS mice, and caution should be taken to its timing when considering using 3 kDa-HA.

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Section: Discussionmentioning

confidence: 99%

Hyaluronan with Different Molecular Weights Can Affect the Gut Microbiota and Pathogenetic Progression of Post-Intensive Care Syndrome Mice in Different Ways

Jiang

Zhu

et al. 2023

IJMS

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“… 55 However, other studies have suggested that Blautia may damage the intestinal barrier. 22 We believe that the relationship of Lactobacillus and Blautia with T2DM is an important direction for future research.…”

Section: Discussionmentioning

confidence: 93%

“… 21 ; de Mooij et al. 22 Bifidobacterium Gram-positive anaerobic bacteria probiotics 1. Inhibit the growth of harmful bacteria, resist the infection of pathogenic bacteria; 2. synthesize vitamins needed by the human body, promote the absorption of minerals by the human body; 3. produce acetic acid, propionic acid, lactic acid, and other organic acids to stimulate intestinal peristalsis; 4. promote defecation, prevent constipation and inhibit intestinal spoilage, purify intestinal environment, decompose carcinogens; 5. stimulate the human immune system, it plays an important role in improving disease resistance.…”

Section: Resultsmentioning

confidence: 99%

Correlation between intestinal flora and GLP-1 receptor agonist dulaglutide in type 2 diabetes mellitus treatment—A preliminary longitudinal study

Liang,

Su,

Guan

et al. 2024

iScience

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“…The findings of our research indicate a positive correlation between the level of plasma sCD14 and Blautia, a gram-positive bacterium. Blautia exhibits the capacity to produce short-chain fatty acids; however, the proportion of genes associated with carbohydrate transport and metabolism in Blautia is relatively small (6.57%), suggesting a limited ability of Blautia to metabolize carbohydrates ( de Mooij et al., 2023 ). Additionally, there exists a disparity in the association of the Blautia genus with human diseases.…”

Section: Discussionmentioning

confidence: 99%

Oral administration of lysozyme protects against injury of ileum via modulating gut microbiota dysbiosis after severe traumatic brain injury

Yang,

Xi,

Yao

et al. 2024

Front. Cell. Infect. Microbiol.

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ObjectiveThe current study sought to clarify the role of lysozyme-regulated gut microbiota and explored the potential therapeutic effects of lysozyme on ileum injury induced by severe traumatic brain injury (sTBI) and bacterial pneumonia in vivo and in vitro experiments.MethodsMale 6–8-week-old specific pathogen-free (SPF) C57BL/6 mice were randomly divided into Normal group (N), Sham group (S), sTBI group (T), sTBI + or Lysozyme-treated group (L), Normal + Lysozyme group (NL) and Sham group + Lysozyme group (SL). At the day 7 after establishment of the model, mice were anesthetized and the samples were collected. The microbiota in lungs and fresh contents of the ileocecum were analyzed. Lungs and distal ileum were used to detect the degree of injury. The number of Paneth cells and the expression level of lysozyme were assessed. The bacterial translocation was determined. Intestinal organoids culture and co-coculture system was used to test whether lysozyme remodels the intestinal barrier through the gut microbiota.ResultsAfter oral administration of lysozyme, the intestinal microbiota is rebalanced, the composition of lung microbiota is restored, and translocation of intestinal bacteria is mitigated. Lysozyme administration reinstates lysozyme expression in Paneth cells, thereby reducing intestinal permeability, pathological score, apoptosis rate, and inflammation levels. The gut microbiota, including Oscillospira, Ruminococcus, Alistipes, Butyricicoccus, and Lactobacillus, play a crucial role in regulating and improving intestinal barrier damage and modulating Paneth cells in lysozyme-treated mice. A co-culture system comprising intestinal organoids and brain-derived proteins (BP), which demonstrated that the BP effectively downregulated the expression of lysozyme in intestinal organoids. However, supplementation of lysozyme to this co-culture system failed to restore its expression in intestinal organoids.ConclusionThe present study unveiled a virtuous cycle whereby oral administration of lysozyme restores Paneth cell’s function, mitigates intestinal injury and bacterial translocation through the remodeling of gut microbiota.

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BlautiaAbundance and Mucosal Barrier Injury: A Complex Play of Cause and Effect

Cited by 7 publications

References 8 publications

Hyaluronan with Different Molecular Weights Can Affect the Gut Microbiota and Pathogenetic Progression of Post-Intensive Care Syndrome Mice in Different Ways

Hyaluronan with Different Molecular Weights Can Affect the Gut Microbiota and Pathogenetic Progression of Post-Intensive Care Syndrome Mice in Different Ways

Correlation between intestinal flora and GLP-1 receptor agonist dulaglutide in type 2 diabetes mellitus treatment—A preliminary longitudinal study

Oral administration of lysozyme protects against injury of ileum via modulating gut microbiota dysbiosis after severe traumatic brain injury

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