Bletilla striata Micron Particles Function as a Hemostatic Agent by Promoting Rapid Blood Aggregation

Abstract

The human body cannot control blood loss without treatment. Available hemostatic agents are ineffective at treating cases of severe bleeding and are expensive or raise safety concerns. Bletilla striata serve as an inexpensive, natural, and promising alternative. However, no detailed studies on its hemostatic approach have been performed. The aim of this study was to examine the hemostatic effects of B. striata Micron Particles (BSMPs) and their hemostatic mechanisms. We prepared and characterized BSMPs of different size ranges and investigated their use as hemostatic agent. BSMPs of different size ranges were characterized by scanning electron microscope. In vitro coagulation studies revealed BSMP-blood aggregate formation via stereoscope and texture analyzers. In vivo studies based on rat injury model illustrated the BSMP capabilities under conditions of hemostasis. Compared to other BSMPs of different size ranges, BSMPs of 350–250 μm are most efficient in hemostasis. As powder sizes decrease, the degree of aggregation between particles and hemostatic BSMP effects declines. The BSMP in contact with a bleeding surface locally forms a visible particle/blood aggregate as a physical barrier that facilitates hemostasis. Considering the facile preparation, low cost, and long shelf life of B. striata, BSMPs offer great potential as mechanisms of trauma treatment.

Selection of Reference Genes for qRT-PCR in Bletilla striata under Heat Stress

“…Modern pharmacology research has further proven that B. striata fights against bacteria (Li et al 2014;Guo et al 2016;Jiang et al 2019), influenza A virus (Shi et al 2017), fibrosis (Wang et al 2014) and tumorigenesis (Zhan et al 2014) and promotes wound (Diao et al 2008;Luo et al 2010) and oral ulcer healing (Liao et al 2019). Additionally, B. striata is used as a hemostatic agent since it promotes rapid blood coagulation (Hung and Wu 2016;Zhang et al 2017). "Yunnan Bai Yao", made mainly from B. striata, has been popular for wound healing for more than a hundred years.…”

De Novo assembly, characterization and development of EST-SSRs from Bletilla striata transcriptomes profiled throughout the whole growing period

“…f. (Orchidaceae) has been widely used as a common medicinal plant and horticulture specimen in many countries for several centuries. The pseudobulbs of this traditional Chinese medicinal (TCM) herb have various medicinal properties, including hemostatic [ 1 ], anti-inflammatory, and antibacterial [ 2 ] activities, as well as the ability to reduce swelling and promote tissue regeneration [ 3 ]. In addition, previous studies also demonstrated that B .…”

Prostatic Artery Embolisation with Bletilla Striata Particles in Treatment of Moderate to Severe Lower Urinary Tract Symptoms (LUTS) Resulted from Benign Prostatic Hyperplasia (BPH)

“…(2) Bletilla striata particles spur hemostatic modes of action by forming a visible particle/blood aggregate as a physical barrier that gives rise to hemostasis. The hemostatic results of 350-250 μm particles were found to be the most efficient [27]. (3) Bletilla striata has the activity anti-inflammatory.…”