Bordetella holmesiiin children suspected of pertussis in Argentina

Bottero, Daniela; Griffith, Matthew; Lara, Claudia; Flores, Daniela; Pianciola, Luis; Gaillard, María Emilia; Mazzeo, M; Zamboni, Michele; Spoleti, M.J.; Anchart, Eduardo; Ruggeri, Domenica Rita; Sorhouet, Cecilia; Fiori, Sandra; Galas, Marcelo; Tondella, M. Lucia; Hozbor, Daniela Flavia

doi:10.1017/s095026881200132x

Cited by 40 publications

(30 citation statements)

References 11 publications

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“…This poses a potential problem, because the diagnosis of B. pertussis infection is based on the PCR detection of IS481 sequences. The development of specific PCR diagnosis for B. holmesii infection, targeting the recA gene [7][8][9], or the transposase IS1001bho [10], the amplification of specific target as bhoE [11] or the sequencing of 16sRNA or OmpA [12] has made it possible for a number of retrospective studies to demonstrate an increase in the number of reported cases of respiratory infections due to B. holmesii over the last few years [10,11,[13][14][15][16][17][18][19][20][21]. We wondered whether this increase in detection reflected a real increase in the number of infections or was simply a consequence of the increasing use of RT-PCR targeting IS481 for the diagnosis of B. pertussis since 2005.…”

Section: Introductionmentioning

confidence: 99%

Bordetella holmesii: Comparison of Two Isolates from Blood and a Respiratory Sample

Bouchez

Guiso²

2013

AID

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Interest in Bordetella holmesii is increasing, but very little is known about this bacterium, which can be isolated from both blood and respiratory samples. In this study, we compared a B. holmesii isolate from the blood sample of an adult with bacteremia with another isolate from a nasopharyngeal swab from an adult with whooping cough syndrome. Genetic analysis was carried out, targeting relevant genes, and virulence properties were studied in cellular and animal models. Our genomic analysis provided no evidence of traits specific to either blood or respiratory isolates of B. holmesii. Neither isolate was cytotoxic to human tracheal epithelial cells. Both isolates were only weakly invasive and they did not persist within epithelial cells for less than 48 h.

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Section: Introductionmentioning

confidence: 99%

Bordetella holmesii: Comparison of Two Isolates from Blood and a Respiratory Sample

Bouchez

Guiso²

2013

AID

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“…Due to the increase in number of notifications of pertussis observed in other countries (16,17) and also in Brazil, for over a decade, there was a need to implement a diagnostic method with higher sensitivity and specificity, with a shorter time to release results. Then, in 2010, we standardized the Real-Time PCR TaqMan® (RT-PCR) in routine diagnosis of Instituto Adolfo Lutz, the national reference center for pertussis in Brazil.…”

Section: Introductionmentioning

confidence: 99%

Implementation and Assessment of the Use of Real-Time PCR in Routine Diagnosis for Bordetella pertussis Detection in Brazil

Leite

Blanco

Melo

et al. 2013

Arch Pediatr Infect Dis

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Background: Bordetella pertussis is the causative agent of pertussis. In Brazil, laboratory diagnosis of pertussis is based on the culture. In 2010, was standardized the Real-Time PCR TaqMan® in routine diagnosis. Objectives: The aim of this study was to evaluate the impact achieved with the introduction of RT-PCR for the routine diagnosis of pertussis and to compare with the results obtained from culture. Patients and Methods: 4,697 samples of nasopharyngeal secretions collected from suspected pertussis cases and/or contacts were analyzed for RT-PCR and culture, from January 2008 until the end of December 2011. Results: According to the results obtained from the samples 6.9% were culture/RT-PCR positive, 14.8% were positive only for RT-PCR and 0.2% only for culture. Negative samples for both techniques was 3,622 (77.1%) and 1.0% were inconclusive for RT-PCR. Conclusions:The implementation of RT-PCR in routine diagnosis resulted in an increase in laboratory confirmation by almost three times. The RT-PCR assay does not intend to replace the culture technique, but to promote an improvement in the diagnosis of pertussis.

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“…This is particularly important for adolescent and adult populations. As previously shown, B. holmesii carriage is mostly found in teenagers and adults, at least in Europe and North America (10,11), although it has been detected in some newborns in Romania, Chile, and Argentina (12)(13)(14). In these populations, it is important to identify the Bordetella species present in the samples, particularly when vaccine effectiveness or the duration of vaccineinduced immunity is determined.…”

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confidence: 99%

Follow-Up of External Quality Controls for PCR-Based Diagnosis of Whooping Cough in a Hospital Laboratory Network (Renacoq) and in Other Hospital and Private Laboratories in France

Guillot

Guiso

2016

J Clin Microbiol

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The French National Reference Centre (NRC) for Whooping Cough carried out an external quality control (QC) analysis in 2010 for the PCR diagnosis of whooping cough. The main objective of the study was to assess the impact of this QC in the participating laboratories through a repeat analysis in 2012.W hooping cough, a highly contagious respiratory disease affecting humans (1), can be diagnosed by culture, PCR, or serology (2). PCR, and particularly real-time PCR (RT-PCR) methods, overcomes some of the limitations of culture and serology and has become the most widely used method for diagnosing Bordetella infection. Insertion sequences IS481 and IS1001 are the most frequently used targets for the detection of B. pertussis and B. parapertussis, respectively. However, the IS481 sequence is also present in the B. holmesii genome (3). Moreover, B. bronchiseptica is causing problems for current consensus molecular diagnostic tests for pertussis, because some isolates harbor IS481 (4) and/or IS1001 (5). Following on the initial proficiency program (6), the French National Reference Centre (NRC) offered quality controls (QCs) to the pediatric hospital laboratory network (7) and some other hospital and private medical laboratories in 2010 (QC-2010) and 2012 (QC-2012. The main objectives were (i) to provide an overview of the PCR methods used by the laboratories and of the interpretation of the data obtained and (ii) to assess the impact of QC-2010 on the participating laboratories by repeat evaluation in 2012. Participation was voluntary, with 33 laboratories participating in QC-2010 and 35 in QC-2012. The participating laboratories were asked to complete a technical questionnaire (Table 1) to provide information about their equipment, reagents, and methods. The DNA samples of Bordetella isolates harboring the IS481 or IS1001 sequence in their genome were prepared by NRC and sent to the participating laboratories (Table 2). Each proficiency panel consisted of six specimens, each containing DNA at a concentration of 0.01 to 100.00 pg/l or molecular biology-quality water. The participants were asked to report the positive or negative result obtained for DNA detection for each tube, together with the crossover threshold (Ct) value and the conclusive sentence routinely used for such situations in their analysis reports. The correct interpretation for a sample that tests positive for IS481 or IS1001 should be "detection of Bordetella species DNA." The conclusive sentence was considered incorrect if the laboratory interpreted a positive test for IS481 as indicating that the sample corresponded to B. pertussis or a positive test for IS1001 as indicating that the sample corresponded to B. parapertussis. The results were compiled and analyzed by NRC and then transmitted to all participants.A summary of the technical questionnaire completed by the laboratories is presented in Table 1. In 2010 and 2012, half of the laboratories routinely used commercial kits, a third used an automated system for DNA extraction from respiratory samp...

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Bordetella holmesiiin children suspected of pertussis in Argentina

Cited by 40 publications

References 11 publications

<i>Bordetella holmesii</i>: Comparison of Two Isolates from Blood and a Respiratory Sample

<i>Bordetella holmesii</i>: Comparison of Two Isolates from Blood and a Respiratory Sample

Implementation and Assessment of the Use of Real-Time PCR in Routine Diagnosis for Bordetella pertussis Detection in Brazil

Follow-Up of External Quality Controls for PCR-Based Diagnosis of Whooping Cough in a Hospital Laboratory Network (Renacoq) and in Other Hospital and Private Laboratories in France

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Bordetella holmesiiin children suspected of pertussis in Argentina

Cited by 40 publications

References 11 publications

&lt;i&gt;Bordetella holmesii&lt;/i&gt;: Comparison of Two Isolates from Blood and a Respiratory Sample

&lt;i&gt;Bordetella holmesii&lt;/i&gt;: Comparison of Two Isolates from Blood and a Respiratory Sample

Implementation and Assessment of the Use of Real-Time PCR in Routine Diagnosis for Bordetella pertussis Detection in Brazil

Follow-Up of External Quality Controls for PCR-Based Diagnosis of Whooping Cough in a Hospital Laboratory Network (Renacoq) and in Other Hospital and Private Laboratories in France

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Product

Resources

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<i>Bordetella holmesii</i>: Comparison of Two Isolates from Blood and a Respiratory Sample

<i>Bordetella holmesii</i>: Comparison of Two Isolates from Blood and a Respiratory Sample