<i>Bordetella pertussis</i>
            Culture Having Only Species Factor 1

Eldering, Grace; Holwerda, Jack; Baker, Julia

doi:10.1128/jb.91.5.1759-1762.1966

Cited by 14 publications

(3 citation statements)

References 7 publications

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“…However, Eldering et al (7,8) were not able to detect a significant difference in active or passive immunization studies with strains of different serotypes, and we, in the present report, could not show mouse protection with agglutinogen 1. However, these observations do not explain the role of these agglutinogen factors in the prophylaxis of whooping cough.…”

Section: Discussioncontrasting

confidence: 77%

Antigens of Bordetella pertussis V. Separation of Agglutinogen 1 and Mouse-Protective Antigen

Ross

Muñoz

1971

Infect Immun

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Agglutinogen I of Bordetella pertussis strain 353/Z (serotype 1) was separated from protective antigen and histamine-sensitizing factor by starch-block electrophoresis. Most of the agglutinogen migrated towards the cathode in starch-block electrophoresis, although some remained near the origin. Fractions containing most of the agglutinogen I were free of detectable mouse-protecting or histamine-sensitizing activities. Agglutinogen 1 from a serotype 1,3 B. pertussis strain (J20) migrated similarly to the agglutinogen I from strain 353/Z. All agglutinogen 3 activity was found at the point of application in the starch block. No clear relationship was found between agglutinogen I and mouse-protecting antigen or histamine-sensitizing factor. Eight different agglutinogens have been described for Bordetella pertussis (1, 5, 6). Six of these (1, 2, 3, 4, 5, 6) are species specific, and two (7, 13) are shared with other species of the genus Bordetella. Agglutinogen 1 is common to all strains of B. pertussis, whereas agglutinogens 2, 3, 4, 5, and 6 are found in various combinations as strain-specific antigens. Agglutinogens 2, 3, and 5 resist 100 C for 2.5 hr, but 1, 6, and 7 are destroyed by this treatment (4). The relationship of these agglutinogens to immunity induced by pertussis vaccines is not clear. Work with purified fractions of B. pertussis has indicated that at least some of the agglutinogens are separated from the antigen that is responsible for immunity in the mouse protection test of Kendrick et al. (10). Moreover, no difference in mouse-protective activity has been demonstrated among vaccines prepared from cultures possessing different combinations of agglutinogens (2, 7, 8, 24). These observations indicate that the specific agglutinogens 2, 3, 4, 5, and 6 do not play a significant role in active immunization of mice. This cannot be said with certainty of agglutinogen 1, because this antigen is present in all smooth strains of B. pertussis (5), and definite separation of this agglutinogen and mouse-protective antigen (PA) has not been reported. Recent epidemiological findings (3, 17) and results of passive mouse protection tests (18,

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Section: Discussioncontrasting

confidence: 77%

Antigens of Bordetella pertussis V. Separation of Agglutinogen 1 and Mouse-Protective Antigen

Ross

Muñoz

1971

Infect Immun

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“…The results of passive protection tests in mice, like those of active tests presented earlier (2). give no indication that protection is associated with serotype.…”

Section: Discussionmentioning

confidence: 71%

Mouse-protective Properties of Bordetella pertussis Serotypes in Passive Tests

1967

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In a passive protection procedure in which the ED50 values of Bordetellc/ pertuessis antisera were determined, groups of mice were given graded intraperitoneal doses of serum, followed the next day by intracerebral challenge with 100,000 organisms. Antiserum produced with B. pertussis culture 5373, serotype 1 3, protected mice against challenge with culture 18-323, serotype 1 2. 3, as effectively as did an antiserum produced with a serotype 1 2.3 culture. When two groups of mice similarly treated with pertussis immune serum were challenged with culture 353Z (serotype 1) and 18-323, respectively, much lower ED50 values were obtained with the animals challenged with 353Z. Passive protection tests with adsorbed antiserum gave equivocal results, suggesting that some of the adsorbing antigen remained in the serum and interfered with the tests. There was no evidence that serotype is related to protection.

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“…Cook County Hospital Laboratory, Chicago, Ill., sent 29 cultures isolated as part of their diagnostic service. The other sources were as follows: Ontario Provincial Laboratory, Windsor, Canada, 11 cultures; Emory University, Atlanta, Ga., 9 cultures; Children's Hospital, Los Angeles, Calif., 8 cultures; Cleveland Metropolitan General Hospital, Cleveland, Ohio, 3 cultures; Maryland State Laboratory, Baltimore, 1 culture.…”

Section: Methodsmentioning

confidence: 99%

Bordetella pertussis Serotypes in the United States

Eldering¹,

Holwerda²,

Davis³

et al. 1969

Applied Microbiology

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To determine the Bordetella pertussis serotypes currently causing whooping cough in the United States, recently isolated cultures from different geographic areas were studied. Specific adsorbed antisera were prepared in our laboratory and used in both tube and slide agglutination tests. Among the 177 cultures isolated during 1966 and 1967 in seven states and one Canadian province, the predominant serotype was 1.3 (or 1.3.6), represented by 85 % of the cultures. Fifteen cultures were serotype 1.2.3.4.6. The 376 cultures isolated in the Grand Rapids area during the last 30 years showed a changing pattern from serotype 1.2.3 during the early years to the currently prevailing 1.3 (or 1.3.6). During the 2-year study period, seven cultures of B. parapertussis were isolated in Michigan, and seven were received from other states. One culture of B. bronchiseptica, recovered from a child in Boston, was sent to us.

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Bordetella pertussis Culture Having Only Species Factor 1

Cited by 14 publications

References 7 publications

Antigens of Bordetella pertussis V. Separation of Agglutinogen 1 and Mouse-Protective Antigen

Antigens of Bordetella pertussis V. Separation of Agglutinogen 1 and Mouse-Protective Antigen

Mouse-protective Properties of Bordetella pertussis Serotypes in Passive Tests

Bordetella pertussis Serotypes in the United States

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