<i>Brachypodium distachyon</i>Cell Suspension Cultures: Establishment and Utilisation

Hunt, D. J.; Chambers, J.P.; Behpouri, A.; Kelly, Sandra; Whelan, Linda C.; Pietrzykowska, M.; Downey, Frances; McCabe, Paul F.; Ng, Carl

doi:10.1556/crc.2013.0040

Cited by 4 publications

(7 citation statements)

References 28 publications

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“…BdbZIP10::GFPo/e callus tissue was homogenized and examined under Nomarski light, and the nucleus was detected with 49,6-diamidino-2-phenylindole (DAPI) staining and UV light (Fig. 6A, sections 1 and 2; Zink et al, 2003;Hunt et al, 2013). Consistent with its potential role as a transcription factor, BdbZIP10::GFP localized to the nucleus at detectable levels (Fig.…”

Section: Bdbzip10 Localizes To the Nucleus With Oxidative Stressmentioning

confidence: 79%

Enhanced Oxidative Stress Resistance through Activation of a Zinc Deficiency Transcription Factor inBrachypodium distachyon

et al. 2014

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Identification of viable strategies to increase stress resistance of crops will become increasingly important for the goal of global food security as our population increases and our climate changes. Considering that resistance to oxidative stress is oftentimes an indicator of health and longevity in animal systems, characterizing conserved pathways known to increase oxidative stress resistance could prove fruitful for crop improvement strategies. This report argues for the usefulness and practicality of the model organism Brachypodium distachyon for identifying and validating stress resistance factors. Specifically, we focus on a zinc deficiency B. distachyon basic leucine zipper transcription factor, BdbZIP10, and its role in oxidative stress in the model organism B. distachyon. When overexpressed, BdbZIP10 protects plants and callus tissue from oxidative stress insults, most likely through distinct and direct activation of protective oxidative stress genes. Increased oxidative stress resistance and cell viability through the overexpression of BdbZIP10 highlight the utility of investigating conserved stress responses between plant and animal systems.

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Section: Bdbzip10 Localizes To the Nucleus With Oxidative Stressmentioning

confidence: 79%

Enhanced Oxidative Stress Resistance through Activation of a Zinc Deficiency Transcription Factor inBrachypodium distachyon

et al. 2014

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“…Seeds of Brachypodium distachyon inbred line Bd21 (kindly provided by Dr. David Garvin, USDA-ARS-Plant Science Research, St. Paul, MN 55108, USA) were germinated and plants were grown as previously described (Hunt et al 2014).…”

Section: Seed Germination and Plant Growthmentioning

confidence: 99%

“…Immature embryos of B. distachyon inbred line Bd21 were used for callus induction 4 to 6 weeks following transfer of the seedlings to soil. The method used for callus induction was according to Vogel and Hill (2008) and Hunt et al (2014). After 3 weeks, the embryos that produced yellow healthy embryogenic callus and those that produced compact embryogenic callus (CEC) were evaluated in each treatment in 3 experimental replicates.…”

Section: Callus Induction and Proliferationmentioning

confidence: 99%

“…Callus induction and formation was conducted according to (Vogel and Hill 2008;Alves et al 2009;Hunt et al 2014). After 6 weeks of embryo culture and embryogenic callus multiplication on LS medium (LS 4.4 g l -1 , 3% (w/v) maltose 2.5 mg l -1 2,4-D, 0.6 mg l -1 CuSO 4 , and 0.8% (w/v) plant cell culture tested agar, pH5.8), CECs were inoculated with A. tumefaciens strain AGL1 (OD 600 = 1) harbouring pPZP-RCS2, pEU334AN, pCAM-BIA 1305.1 in LS medium supplemented with 45 mg l -1 acetosyringone (AS).…”

Section: Transformation Of B Distachyon Calli Using Agrobacterium Tumentioning

confidence: 99%

“…They used 2.5mg l -1 2,4-D for callus initiation. Hunt et al (2014) established B. distachyon cell suspension cultures using 7.5 mg l -1 2,4-D for callus initiation. Due to inter-laboratory differences, it is important to empirically determine the optimal conditions for callus induction and proliferation before Agrobacterium-mediated transformation.…”

Section: Introductionmentioning

confidence: 99%

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Optimizing callus induction and proliferation for Agrobacterium-mediated transformation of Brachypodium distachyon

Behpouri

Perochon

Doohan

et al. 2018

Cereal Research Communications

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Brachypodium distachyon has emerged as the model species for important temperate grass crops such as wheat and barley and the genome of the B. distachyon community inbred line Bd21 has been sequenced. Methods for tissue culture and Agrobacterium-mediated transformation have been developed for this model grass as a resource for reverse genetics and functional genomic analyses. In order to obtain a high quantity and quality of compact embryogenic callus (CEC) in B. distachyon, it is important to examine and optimize the optimal concentration of the auxin 2,4-D (dichlorophenoxyacetic acid) to use in both callus induction and callus proliferation media. Here, we investigated the effects of different concentrations of 2,4-D on callus induction and callus proliferation of B. distachyon Bd21. Our results showed that 2.5 mg l-1 2,4-D is an optimal concentration to use for both callus induction and proliferation, although 5.0 mg l-1 may also be used for callus proliferation. Additionally, the suitability of hygromycin or bialaphos as selectable markers was examined and results indicated that hygromycin is significantly more efficient than bialaphos when using the Agrobacterium-mediated transformation system.

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A simple method for the isolation of leaf epidermis from graminaceous species for studying stomatal physiology

Brett

2021

CEREAL RESEARCH COMMUNICATIONS

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Brachypodium distachyonCell Suspension Cultures: Establishment and Utilisation

Cited by 4 publications

References 28 publications

Enhanced Oxidative Stress Resistance through Activation of a Zinc Deficiency Transcription Factor inBrachypodium distachyon

Enhanced Oxidative Stress Resistance through Activation of a Zinc Deficiency Transcription Factor inBrachypodium distachyon

Optimizing callus induction and proliferation for Agrobacterium-mediated transformation of Brachypodium distachyon

A simple method for the isolation of leaf epidermis from graminaceous species for studying stomatal physiology

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