<i>Bradyrhizobium iriomotense</i>sp. nov., Isolated from a Tumor-Like Root of the Legume<i>Entada koshunensis</i>from Iriomote Island in Japan

Islam, Muhammad Saiful; Kawasaki, Hiroko; Muramatsu, Yuki; Nakagawa, Yasuyoshi; Seki, Tatsuji

doi:10.1271/bbb.70739

Cited by 77 publications

(34 citation statements)

References 44 publications

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“…The dominant fatty acid for strain CCBAU 23068 T was C 18 : 1 v7c. This was different to the dominant fatty acid found in the three related species, but was consistent with the major fatty acid found for other species of the genus Bradyrhizobium, such as B. japonicum and Bradyrhizobium liaoningense (Islam et al, 2008). Several fatty acids (C 12 : 0 2-OH, C 14 : 0 iso 3-OH, C 15 : 1 iso F and C 17 : 0 2-OH) were found in small amounts only in strain CCBAU 23086 T of the novel group.…”

supporting

confidence: 81%

Bradyrhizobium lablabi sp. nov., isolated from effective nodules of Lablab purpureus and Arachis hypogaea

Chang

Wang

et al. 2011

International Journal of Systematic and Evolutionary Microbiology

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Five strains isolated from root nodules of Lablab purpureus and Arachis hypogaea grown in the Anhui and Sichuan provinces of China were classified as members of the genus Bradyrhizobium. These strains had identical 16S rRNA gene sequences which shared 99.48 %, 99.48 % and 99.22 % similarity with the most closely related strains of Bradyrhizobium jicamae PAC68 T , Bradyrhizobium pachyrhizi PAC48 T and Bradyrhizobium elkanii USDA 76 T , respectively. A study using a polyphasic approach, including 16S rRNA gene RFLP, IGS-RFLP, BOX-PCR, comparative sequence analysis of the 16S-23S rRNA intergenic spacer (IGS) and the recA, atpD and glnII genes, DNA-DNA hybridization and phenotypic tests, showed that the five strains clustered into a coherent group that differentiated them from all recognized species of the genus Bradyrhizobium. Sequencing of nifH and nodC genes and cross-nodulation tests showed that the representative strains CCBAU 23086 T , CCBAU 23160 and CCBAU 61434, isolated from different plants, had identical nifH and nodC gene sequences and were all able to nodulate Lablab purpureus, Arachis hypogaea and Vigna unguiculata. Based upon these results, the name Bradyrhizobium lablabi sp. nov. is proposed for this novel species and strain CCBAU 23086 T (5LMG 25572 T 5HAMBI 3052 T ) is designated as the type strain. The DNA G+C mol% is 60.14 (T m ).

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supporting

confidence: 81%

Bradyrhizobium lablabi sp. nov., isolated from effective nodules of Lablab purpureus and Arachis hypogaea

Chang

Wang

et al. 2011

International Journal of Systematic and Evolutionary Microbiology

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“…in China, and B. betae from tumor-like root deformation on sugar-beet in Northern Spain [30]. Three new species, designated B. pachyrhiziand B.jicamae, isolated from the legume Pachyrhizuserosus [29], and B. iriomotense, isolated from a root outgrowth of Entada koshunensis, a legume available in Japan [18], have recently been proposed. Additionally, the photosynthetic bradyrhizobia and two other evolutionary lineages, labeled as genospecies alpha and beta, represent unnamed species that may include a greater number of bradyrhizobia isolates not yet characterized [48].…”

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confidence: 99%

Endosymbiotic bacteria nodulating a new endemic lupine Lupinus mariae-josephi from alkaline soils in Eastern Spain represent a new lineage within the Bradyrhizobium genus

Sánchez-Cañizares

Rey

Durán

et al. 2011

Systematic and Applied Microbiology

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Lupinus mariae-josephi isa recently described endemic Lupinus species from a small area in Eastern Spain where it thrives in soils with active lime and high pH. The L. mariae-josephi root symbionts were shown to be very slow-growing bacteria with different phenotypic and symbiotic characteristics from those of Bradyrhizobium strains nodulating other Lupinus. Their phylogenetic status was examined by multilocus sequence analyses of four housekeeping genes (16S rRNA, glnl I, recA, and atpD) and showed the existence of a distinct evolutionary lineage for L. mariae-josephi thatalso included Bradyrhizobiumjicamae. Within this lineage, the tested isolates clustered in three different sub-groups that might correspond to novel sister Bradyrhizobium species. These core gene analyses consistently showed that all the endosymbiotic bacteria isolated from other Lupinus species of the Iberian Peninsula were related to strains of the B. canariense or B. japonicum lineages and were separate from the L. mariae-josephi isolates. Phylogenetic analysis based on nodC symbiotic gene sequences showed that L. mariae-josephi bacteria also constituted a new symbiotic lineage distant from those previously defined in the genus Bradyrhizobium. In contrast, the nodC genes of isolates from other Lupinus spp. from the Iberian Peninsula were again clearly related to the B. canariense and B. japonicum bv. genistearum lineages. Speciation of L. mariae-josephi bradyrhizobia may result from the colonization of a singular habitat by their unique legume host.

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“…Amplification and sequencing of recA, atpD, dnaK and thrC genes were carried out according to the methods of Islam et al (2008). The obtained sequences were compared with those from GenBank using the BLASTN program (Altschul et al, 1990) …”

mentioning

confidence: 99%

Rhizobium azibense sp. nov., a nitrogen fixing bacterium isolated from root-nodules of Phaseolus vulgaris

Mnasri

Liu

Saïdi

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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Three microbial strains isolated from common beans, 23C2T (Tunisia), Gr42 (Spain) and IE4868 (Mexico), which have been identified previously as representing a genomic group closely related to Rhizobium gallicum , are further studied here. Their 16S rRNA genes showed 98.5–99 % similarity with Rhizobium loessense CCBAU 7190BT, R. gallicum R602spT, Rhizobium mongolense USDA 1844T and Rhizobium yanglingense CCBAU 71623T. Phylogenetic analysis based on recA, atpD, dnaK and thrC sequences showed that the novel strains were closely related and could be distinguished from the four type strains of the closely related species. Strains 23C2T, Gr42 and IE4868 could be also differentiated from their closest phylogenetic neighbours by their phenotypic and physiological properties and their fatty acid contents. All three strains harboured symbiotic genes specific to biovar gallicum. Levels of DNA–DNA relatedness between strain 23C2T and the type strains of R. loessense , R. mongolense , R. gallicum and R. yanglingense ranged from 58.1 to 61.5 %. The DNA G+C content of the genomic DNA of strain 23C2T was 59.52 %. On the basis of these data, strains 23C2T, Gr42 and IE4868 were considered to represent a novel species of the genus Rhizobium for which the name Rhizobium azibense is proposed. Strain 23C2T ( = CCBAU 101087T = HAMBI3541T) was designated as the type strain.

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Bradyrhizobium iriomotensesp. nov., Isolated from a Tumor-Like Root of the LegumeEntada koshunensisfrom Iriomote Island in Japan

Cited by 77 publications

References 44 publications

Bradyrhizobium lablabi sp. nov., isolated from effective nodules of Lablab purpureus and Arachis hypogaea

Bradyrhizobium lablabi sp. nov., isolated from effective nodules of Lablab purpureus and Arachis hypogaea

Endosymbiotic bacteria nodulating a new endemic lupine Lupinus mariae-josephi from alkaline soils in Eastern Spain represent a new lineage within the Bradyrhizobium genus

Rhizobium azibense sp. nov., a nitrogen fixing bacterium isolated from root-nodules of Phaseolus vulgaris

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