<i>Caenorhabditis elegans </i><scp>DLC</scp>‐1 associates with ribonucleoprotein complexes to promote <scp>mRNA</scp> regulation

Day, Nicholas; Ellenbecker, Mary; Voronina, Ekaterina

doi:10.1002/1873-3468.13259

Cited by 2 publications

(2 citation statements)

References 59 publications

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“…The other GLD-1 RNPs are capable of regulating their specific targets independent of DLC-1. In support of this model, a subset of GLD-1 target mRNAs recovered through DLC-1 immunoprecipitation includes cye-1 and mes-3 mRNAs, but excludes puf-5 and spn-4 mRNAs (Day et al 2018). In the future, it would be of interest to identify GLD-1 target mRNAs that are misregulated in the gld-1 ndb ::ollas mutant worms leading to disruption of gametogenesis and oocyte cell cycle control or ovulation.…”

Section: Dlc-1 Cooperation With Gld-1 Is Consistent With Allosteric Ementioning

confidence: 85%

“…At the restrictive temperature (25°) gld-1(op236) germline exhibits enhanced cell death phenotype and only a small number of cells are able to exit pachytene and produce oocytes (Schumacher et al 2005). At 20°, dlc-1(-) mutant worms are sterile and the predominant phenotypes in the germline are enlarged germ cell nuclei and rapid deterioration of forming oocytes at the proximal end of the gonad resulting in only a few diplotene nuclei observed ( Figure S2B; Dorsett and Schedl 2009;Day et al 2018). A subset of both dlc-1(-) and gld-1(op236); dlc-1(-) double mutant worms exhibit a disorganized germline phenotype where some cells that fail to exit pachytene are interspersed with cells forming oocytes at 20°( Figure S2, B-D).…”

Section: Dlc-1/gld-1 Cooperation Facilitates Meiotic Entry and Prevenmentioning

confidence: 99%

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Dynein Light Chain DLC-1 Facilitates the Function of the Germline Cell Fate Regulator GLD-1 in Caenorhabditis elegans

Ellenbecker¹,

Osterli²,

Wang³

et al. 2018

Genetics

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Developmental transitions of germ cells are often regulated at the level of post-transcriptional control of gene expression. In the Caenorhabditis elegans germline, stem and progenitor cells exit the proliferative phase and enter meiotic differentiation to form gametes essential for fertility. The RNA binding protein GLD-1 is a cell fate regulator that promotes meiosis and germ cell differentiation during development by binding to and repressing translation of target messenger RNAs. Here, we discovered that some GLD-1 functions are promoted by binding to DLC-1, a small protein that functions as an allosteric regulator of multisubunit protein complexes. We found that DLC-1 is required to regulate a subset of GLD-1 target messenger RNAs and that DLC-1 binding GLD-1 prevents ectopic germ cell proliferation and facilitates gametogenesis in vivo. Additionally, our results reveal a new requirement for GLD-1 in the events of oogenesis leading to ovulation. DLC-1 contributes to GLD-1 function independent of its role as a light chain component of the dynein motor. Instead, we propose that DLC-1 promotes assembly of GLD-1 with other binding partners, which facilitates formation of regulatory ribonucleoprotein complexes and may direct GLD-1 target messenger RNA selectivity.

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Section: Dlc-1 Cooperation With Gld-1 Is Consistent With Allosteric Ementioning

confidence: 85%

Section: Dlc-1/gld-1 Cooperation Facilitates Meiotic Entry and Prevenmentioning

confidence: 99%

Dynein Light Chain DLC-1 Facilitates the Function of the Germline Cell Fate Regulator GLD-1 in Caenorhabditis elegans

Ellenbecker¹,

Osterli²,

Wang³

et al. 2018

Genetics

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DLC-1 facilitates germ granule assembly in Caenorhabditis elegans embryo

Day

Ellenbecker

Wang

et al. 2022

MBoC

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Germ granules are cytoplasmic assemblies of RNA binding proteins (RBPs) required for germ cell development and fertility. During the first four cell divisions of Caenorhabditis elegans zygote, regulated assembly of germ (P) granules leads to their selective segregation to the future germ cell. Here we investigate the role of DLC-1, a hub protein implicated in stabilization and function of diverse protein complexes, in maintaining P granule integrity. We find that DLC-1 directly interacts with several core P granule proteins, predominantly during embryogenesis. The loss of dlc-1 disrupts assembly of P granule components into phase-separated organelles in the embryos, regardless of whether or not DLC-1 directly interacts with these proteins. Finally, we infer that P granule dispersal in the absence of dlc-1 is likely independent of DLC-1’s function as a subunit of the dynein motor and does not result from a loss of cell polarity.

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Caenorhabditis elegans DLC‐1 associates with ribonucleoprotein complexes to promote mRNA regulation

Cited by 2 publications

References 59 publications

Dynein Light Chain DLC-1 Facilitates the Function of the Germline Cell Fate Regulator GLD-1 in Caenorhabditis elegans

Dynein Light Chain DLC-1 Facilitates the Function of the Germline Cell Fate Regulator GLD-1 in Caenorhabditis elegans

DLC-1 facilitates germ granule assembly in Caenorhabditis elegans embryo

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