Calluna vulgaris (L.) Hull: chemical characterization, evaluation of its bioactive properties and effect on the vaginal microbiota

Abstract

C. vulgaris inflorescences revealed myricetin derivatives as the main compound and polar extracts revealed higher bioactivities, preserving vaginal microbiota.

Chemical composition and in vitro biological activities of cardoon (Cynara cardunculus L. var. altilis DC.) seeds as influenced by viability

“…To the best of the authors' knowledge, this is the first study comparing the antioxidant activity of viable and unviable cardoon seeds and measuring their (antihaemolytic) activity by OxHLIA. A correlation between the content of phenolic compounds and antioxidant properties has been demonstrated (Mandim et al, 2019;Palmeira et al, 2019;Petropoulos, Fernandes, et al, 2018). Herein, viable seeds revealed higher levels of phenolic compounds and α-tocopherol and a higher antioxidant capacity, thus suggesting a positive correlation between these parameters.…”

“…An erythrocyte solution (2.8%, v/v; 200 µL) was mixed with 400 µL of either extract solution (18.75-600 µg/mL in PBS), PBS (control), or water (for complete haemolysis). After pre-incubation at 37°C for 10 min with shaking, AAPH (200 μL, 160 mM in PBS) was added and the optical density (690 nm) was measured every 10 min in a microplate reader (Bio-Tek Instruments Inc., ELX800, Winooski, VT, USA) until complete haemolysis (Mandim et al, 2019). The results were expressed as IC 50 values (µg/mL) at Δt of 60 min and 120 min.…”

“…The extracts capacity to inhibit the lipopolysaccharide (LPS)-induced NO production by a murine macrophage cell line (RAW 264.7) was determined as nitrite concentration in the culture medium as described previously (Mandim et al, 2019). Extracts re-dissolved in DMSO at a final concentration of 8 mg/mL were tested.…”

Changes in the Content of Some Groups of Phenolic Compounds and Biological Activity of Extracts of Various Parts of Heather (Calluna vulgaris (L.) Hull) at Different Growth Stages

“…The wide variety of medicinal properties of heather is determined by the biologically active substances present, especially the phenolic compounds [17].…”

Effect of Natural Preservatives on the Nutritional Profile, Chemical Composition, Bioactivity and Stability of a Nutraceutical Preparation of Aloe arborescens

“…The OxHLIA assay followed a procedure described previously by Mandim et al [18] in which a known weight of extract was dissolved in phosphate buffered saline solution (PBS) (Thermo Fisher Scientific Co., Waltham, MS, USA), obtaining different concentrations ranging from 10 µg mL −1 to 25 mg mL −1 . To determine the inhibition capacity of the oxidative hemolysis associated with each of the tested extracts, blood from healthy sheep was harvested and the isolated erythrocytes were used as substrate.…”