<i>Campylobacter</i>-Induced Interleukin-8 Secretion in Polarized Human Intestinal Epithelial Cells Requires<i>Campylobacter</i>-Secreted Cytolethal Distending Toxin- and Toll-Like Receptor-Mediated Activation of NF-κB

Zheng, Jie; Meng, Jianghong; Zhao, Shaohua; Singh, Ruby; Song, Wenxia

doi:10.1128/iai.01317-07

Cited by 113 publications

(88 citation statements)

References 60 publications

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“…Furthermore, we have identified mmp9 and irak3 as novel MyD88-dependent immune response genes. MyD88-dependent as well as MyD88-independent induction of IL-8 has been observed in mammalian systems (57,58). However, regarding the Myd88-independent il8 induction that we observed in zebrafish embryos, note that the orthology of this gene with mammalian IL-8 is ambiguous and that zebrafish express other closely related putative IL-8 homologs.…”

Section: Discussioncontrasting

confidence: 45%

Transcriptome Profiling and Functional Analyses of the Zebrafish Embryonic Innate Immune Response to Salmonella Infection

Stockhammer

Zakrzewska

Hegedűs

et al. 2009

The Journal of Immunology

197

204

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Due to the clear separation of innate immunity from adaptive responses, the externally developing zebrafish embryo represents a useful in vivo model for identification of innate host determinants of the response to bacterial infection. Here we performed a time-course transcriptome profiling study and gene ontology analysis of the embryonic innate immune response to infection with two model Salmonella strains that elicit either a lethal infection or an attenuated response. The transcriptional response to infection with both the lethal strain and the avirulent LPS O-Ag mutant strain showed clear conservation with host responses detected in other vertebrate models and human cells, including induction of genes encoding cell surface receptors, signaling intermediates, transcription factors, and inflammatory mediators. Furthermore, our study led to the identification of a large set of novel immune response genes and infection markers, the future functional characterization of which will support vertebrate genome annotation. From the time series and bacterial strain comparisons, matrix metalloproteinase genes, including mmp9, were among the most consistent infection-responsive genes. Purified Salmonella flagellin also strongly induced mmp9 expression. Using knockdown analysis, we showed that this gene was downstream of the zebrafish homologs of the flagellin receptor TLR5 and the adaptor MyD88. Additionally, flagellin-mediated induction of other inflammation markers, including il1b, il8, and cxcl-C1c, was reduced upon Tlr5 knockdown as well as expression of irak3, a putative negative TLR pathway regulator. Finally, we showed that induction of il1b, mmp9, and irak3 requires Myd88-dependent signaling, while ifn1 and il8 were induced Myd88 independently during Salmonella infection.

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Section: Discussioncontrasting

confidence: 45%

Transcriptome Profiling and Functional Analyses of the Zebrafish Embryonic Innate Immune Response to Salmonella Infection

Stockhammer

Zakrzewska

Hegedűs

et al. 2009

The Journal of Immunology

197

204

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“…Infection of fully differentiated T84 cells with C. jejuni upregulated DCs and T-cell chemokine gene transcription and secretion through the activation of NF-B signaling, but this is independent of TLR5 activation by flagellin (725). C. jejuni, by use of its flagellum and cytolethal distending toxin, increases the secretion of both IL-8 and TNF-␣ via TLR4-dependent NF-B signaling (726). C. jejuni isolates expressing ganglioside-like LOS increase the secretion of the T-cell attractant CXCL10 in fully differentiated Caco-2 and T84 cells (348).…”

Section: Host Cellular Defense Responsesmentioning

confidence: 99%

Pathogenesis of Human Enterovirulent Bacteria: Lessons from Cultured, Fully Differentiated Human Colon Cancer Cell Lines

Moal

Servin

2013

Microbiol Mol Biol Rev

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SUMMARY Hosts are protected from attack by potentially harmful enteric microorganisms, viruses, and parasites by the polarized fully differentiated epithelial cells that make up the epithelium, providing a physical and functional barrier. Enterovirulent bacteria interact with the epithelial polarized cells lining the intestinal barrier, and some invade the cells. A better understanding of the cross talk between enterovirulent bacteria and the polarized intestinal cells has resulted in the identification of essential enterovirulent bacterial structures and virulence gene products playing pivotal roles in pathogenesis. Cultured animal cell lines and cultured human nonintestinal, undifferentiated epithelial cells have been extensively used for understanding the mechanisms by which some human enterovirulent bacteria induce intestinal disorders. Human colon carcinoma cell lines which are able to express in culture the functional and structural characteristics of mature enterocytes and goblet cells have been established, mimicking structurally and functionally an intestinal epithelial barrier. Moreover, Caco-2-derived M-like cells have been established, mimicking the bacterial capture property of M cells of Peyer's patches. This review intends to analyze the cellular and molecular mechanisms of pathogenesis of human enterovirulent bacteria observed in infected cultured human colon carcinoma enterocyte-like HT-29 subpopulations, enterocyte-like Caco-2 and clone cells, the colonic T84 cell line, HT-29 mucus-secreting cell subpopulations, and Caco-2-derived M-like cells, including cell association, cell entry, intracellular lifestyle, structural lesions at the brush border, functional lesions in enterocytes and goblet cells, functional and structural lesions at the junctional domain, and host cellular defense responses.

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“…12 Zehng et al demonstrated that Campylobacter infection is accompanied by the elevation of IL-8 and TNF α, which could also play an important role in the initiation of malignant transformation. 32 It seems that changing environmental conditions in the esophagus, for example exposure to acidic gastric contents, favors the colonization of the gastrointestinal tract by C. concisus. The original change, however, has not as yet been established and requires further studies.…”

Section: Concisus and Esophagus Diseasesmentioning

confidence: 99%

“…26,31 NF-κ B is a major factor controlling the activity of pro-inflammatory factors in epithelial cells. 32 Activation of the NF-κ B path is equivalent to increased levels of proinflammatory cytokines like Il-1, IL-6, IL-8 or TNF α, contributes directly to stimulating an immune response and influences the rate of neoplastic transformation. 26,31 Type II microbiome could be used as an indicator of the need for intervention in daily practice if it were proved that its presence contributes to the progression of erosive esophageal cancer.…”

Section: Concisus and Esophagus Diseasesmentioning

confidence: 99%

Campylobacter concisus as the etiologic agent of gastrointestinal diseases

Akutko¹,

Matusiewicz²

2017

Adv Clin Exp Med

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A number of reports on the pathogenic influence of Campylobacter concisus on the human body and its role in many diseases of the gastrointestinal system, including gastroesophageal reflux disease, Barrett's esophagus and inflammatory bowel disease, have appeared lately. Campylobacter concisus is a Gram negative bacteria which requires an anaerobic environment or microaerophilic environment with hydrogen for growth and is therefore difficult to culture. Due to this difficulty, the rate of infections in epidemiological data are underestimated. There are reports that C. concisus was the only pathogen isolated from the stool of patients with acute diarrhea, which could indicate that it is an etiologic factor of acute gastrointestinal infections in humans. Moreover, the results of some studies suggest that infection with C. concisus is a factor predisposing to the development of gastroesophageal reflux disease and Barrett's esophagus, conditions which may be present before the development of cancer. There are also studies which indicate C. concisus infection as a trigger of inflammatory bowel disease, since it has been demonstrated that C. concisus is present more frequently in patients with newly diagnosed Crohn's disease than in a control group.

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Campylobacter-Induced Interleukin-8 Secretion in Polarized Human Intestinal Epithelial Cells RequiresCampylobacter-Secreted Cytolethal Distending Toxin- and Toll-Like Receptor-Mediated Activation of NF-κB

Cited by 113 publications

References 60 publications

Transcriptome Profiling and Functional Analyses of the Zebrafish Embryonic Innate Immune Response to Salmonella Infection

Transcriptome Profiling and Functional Analyses of the Zebrafish Embryonic Innate Immune Response to Salmonella Infection

Pathogenesis of Human Enterovirulent Bacteria: Lessons from Cultured, Fully Differentiated Human Colon Cancer Cell Lines

Campylobacter concisus as the etiologic agent of gastrointestinal diseases

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