<i>Candida</i>-Specific Antibodies during Experimental Vaginal Candidiasis in Mice

Wozniak, Karen L.; Wormley, Floyd L.; Fidel, Paul L.

doi:10.1128/iai.70.10.5790-5799.2002

Cited by 48 publications

(25 citation statements)

References 50 publications

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“…Animals were divided into 3 major groups per models. In vaginal infection model, 50 µl subcutaneous estradiol-valerate (10 mg/ml) was given to all mice three days prior to infection [11][12][13].…”

Section: Methodsmentioning

confidence: 99%

Serum interleukin-6 levels in murine models of Candida albicans infection

Kovács

Czudar

Horváth

et al. 2014

Acta Microbiologica Et Immunologica Hungarica

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Two Balb/C mouse models of Candida infection were used to detect serum interleukin-6 (IL-6) responses. The first model used systemic infection by Candida albicans ATCC 10231 strain infected through the lateral tail vein of mice without any specific pretreatment. The median Candida burdens of the kidneys were 1.5 × 106 CFU/ml 24 h postinoculation (p.i.) and 1.2 × 107 CFU/ml 72 h p.i., while median serum IL-6 levels were 479.3 pg/ml and 934.5 pg/ml, respectively. The Candida burden showed significant correlation with serum IL-6 24 h p.i. (R2 = 0.6358; P = 0.0082) but not 72 h p.i.The second model was a mouse vaginitis model applying intravaginal inoculation of mice pretreated with subcutaneous estradiol-valerate (10 mg/ml) 3 days before infection. Candida cell count in vaginal lavage fluid was 2.8 × 106 CFU/ml 24 h p.i. and 1.4 × 108 CFU/ml 72 h p.i. Serum IL-6 response was detected in 4 of 15 mice 24 h p.i. and 9 of 15 mice 72 h p.i. Even the responders had low IL-6 serum levels (mean values 29.9 pg/ml and 60.1 pg/ml, respectively) not correlating with Candida cell count in vaginal lavage fluid.In conclusion, serum IL-6 had strong relationship with systemic C. albicans infection while the local C. albicans infection of the vagina led to partial, prolonged and limited serum IL-6 response.

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Section: Methodsmentioning

confidence: 99%

Serum interleukin-6 levels in murine models of Candida albicans infection

Kovács

Czudar

Horváth

et al. 2014

Acta Microbiologica Et Immunologica Hungarica

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“…[77][78][79] More recently a comprehensive evaluation of Candida-specific IgG and IgA including subclasses (IgG1-4, IgA1-2) and secretory IgA (sIgA) in saliva showed no differences between HIV-negative and HIV-positive persons with or without OPC. 80 Therefore there appears little evidence for any deficiency or over expression of Candida-specific antibodies that could account for susceptibility to OPC.…”

Section: Local Immunitymentioning

confidence: 99%

Oral Opportunistic Infections in HIV-Positive Individuals: Review and Role of Mucosal Immunity

Leigh

Shetty

Fidel

2004

AIDS Patient Care and STDs

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Oral opportunistic infections in the HIV-positive individual have been documented since the first reports of the epidemic, with many lesions associated with reduced CD4(+) T lymphocyte cell count. The most common oral lesions seen in HIV disease prior to the advent of highly active antiretroviral therapy (HAART) were oropharyngeal candidiasis and oral hairy leukoplakia. However, since the advent of HAART while many oral lesions have decreased significantly the incidence of oral warts has surprisingly increased. Despite the correlation of diminished CD4(+) T lymphocyte count to the occurrence of these lesions, it is rare for the lesions to occur concurrently suggesting that each pathologic lesion type is associated with distinct host immune dysfunctions. To date, the oral opportunistic infection most frequently investigated is oropharyngeal candidiasis, where data suggests that both systemic and local immunity is important for protection against infection. In contrast, recent investigations into the host responses associated with oral hairy leukoplakia and oral warts show little to no evidence of systemic or mucosal immune responsiveness despite the presumed competence of several types of leukocytes other than CD4(+) T cells. Together these data are suggesting that susceptibility to oropharyngeal candidasis in HIV-positive persons is predominantly immune-based, whereas protection or susceptibility to oral hairy leukoplakia and oral warts may be more associated with factors other than mucosal immune function.

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“…For the model of VVC, female CBA/J (H-2 k ) mice in pseudoestrus were intravaginally inoculated with 5 ϫ 10 4 CFU of the C. albicans strains (26). We detected no differences in morphology or tissue burdens between CAI-12 and the null mutant in vaginal lavages recovered on days 4 and 7 postinfection (log CFU/ml for CAI-12, 3.34 Ϯ 0.35 at day 4 and 3.13 Ϯ 0.34 at day 7; log CFU/ml for the null mutant, 3.96 Ϯ 0.53 at day 4 and 2.54 Ϯ 0.89 at day 7) (P ϭ NS for both days).…”

Section: Vol 73 2005mentioning

confidence: 99%

The Role of Candida albicans NOT5 in Virulence Depends upon Diverse Host Factors In Vivo

Cheng¹,

Clancy

Checkley

et al. 2005

Infect Immun

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We previously identified Candida albicans Not5p as an immunogenic protein expressed during oropharyngeal candidiasis (OPC). In this study, we demonstrate that C. albicans NOT5 reverses the growth defects of a Saccharomyces cerevisiae not5 mutant strain at 37°C, suggesting that the genes share at least some functional equivalence. We implicate C. albicans NOT5 in the pathogenesis of disseminated candidiasis (DC) induced by intravenous infection among neutropenic and nonimmunosuppressed mice, as well as in that of OPC in mice immunosuppressed with corticosteroids. We find no role in virulence, however, among neutropenic and corticosteroid-suppressed mice with DC resulting from gastrointestinal translocation, nor do we implicate the gene in vulvovaginal candidiasis among mice in pseudoestrus. These findings suggest that the role of NOT5 in virulence depends on the specific in vivo environment and is influenced by diverse factors such as tissue site, portal of entry, and the status of host defenses. NOT5 is necessary for normal adherence to colonic and cervical epithelial cells in vitro, demonstrating that such assays cannot fully replicate disease processes in vivo. Lastly, antibody responses against Not5p do not differ in the sera of patients with OPC, patients with DC, and healthy controls, suggesting that the protein is associated with both commensalism and the pathogenesis of disease.

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Candida-Specific Antibodies during Experimental Vaginal Candidiasis in Mice

Cited by 48 publications

References 50 publications

Serum interleukin-6 levels in murine models of Candida albicans infection

Serum interleukin-6 levels in murine models of Candida albicans infection

Oral Opportunistic Infections in HIV-Positive Individuals: Review and Role of Mucosal Immunity

The Role of Candida albicans NOT5 in Virulence Depends upon Diverse Host Factors In Vivo

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