Candida siamensissp. nov., an anamorphic yeast species in theSaturnisporaclade isolated in Thailand

Abstract

Three strains of a novel anamorphic yeast species were isolated from natural samples collected in Thailand, including strain EF10 isolated from sediment in a mangrove forest, strain ST-473 from rotten wood, and strain ST-479 from a wild mushroom (Hygrophorus sp.). Analysis of the D1/D2 domains of the large-subunit rRNA gene sequence revealed that the sequence of strain EF10 differed from the other two strains (ST-473 and ST-479), which had identical sequences, by only one nucleotide (nt) substitution in 535 nt. The closest species in terms of pairwise sequence similarity was Saturnispora mendoncae, with 32 nt substitutions and seven gaps in 535 nt. Phylogenetic analysis demonstrated that the three strains form a cluster with S. mendoncae and the other eight species in the Saturnispora clade. Phenotypic characteristics of the three strains were the same including proliferation by multilateral budding, absence of ascospores and negative diazonium blue B and urease reactions. The major ubiquinone was Q-7. On this basis, the three strains were assigned to a single novel species of the genus Candida, for which the name Candida siamensis sp. nov. is proposed. The type strain is EF10(T) (=BCC 29901(T)=NBRC 104878(T)=CBS 11022(T)).

Spathaspora allomyrinae sp. nov., a d-xylose-fermenting yeast species isolated from a scarabeid beetle Allomyrina dichotoma

“…Species described later in the genus Spathaspora include Spathaspora arborariae (Cadete et al, 2009), Spathaspora brasiliensis, Spathaspora roraimanensis, Spathaspora suhii and Spathaspora xylofermentans (Cadete et al, 2013). Five anamorphic Candida species, namely Candida jeffriesii, Candida lyxosophila, Candida materiae, Candida subhashii and Candida xylanilytica, are also related to this genus as indicated by phylogenetic analyses of the D1/D2 domains of the large subunit (LSU) rRNA gene (Boonmak et al, 2011;Cadete et al, 2013). Species of the Spathaspora clade are associated with rotting wood or insects linked with this substrate (Boonmak et al, 2011;Cadete et al, 2009Cadete et al, , 2013Nguyen et al, 2011).…”

“…Five anamorphic Candida species, namely Candida jeffriesii, Candida lyxosophila, Candida materiae, Candida subhashii and Candida xylanilytica, are also related to this genus as indicated by phylogenetic analyses of the D1/D2 domains of the large subunit (LSU) rRNA gene (Boonmak et al, 2011;Cadete et al, 2013). Species of the Spathaspora clade are associated with rotting wood or insects linked with this substrate (Boonmak et al, 2011;Cadete et al, 2009Cadete et al, , 2013Nguyen et al, 2011). Most species of the clade, such as C. jeffriesii, C. lyxosophila, Spathaspora arborariae, Spathaspora brasiliensis, Spathaspora passalidarum, Spathaspora roraimanensis, Spathaspora suhii and Spathaspora xylofermentans, are known for their ability to ferment D-xylose.…”

Saturnispora serradocipensis sp. nov. and Saturnispora gosingensis sp. nov., two ascomycetous yeasts from ephemeral habitats

“…Six anamorphic Candida species, C. diversa, C. sanitii, C. sekii, C. siamensis, C. silvae and C. suwanaritii, are also related to this clade (Morais et al 2005;Kurtzman 2006;Boonmak et al 2009;Limtong et al 2010). Most species of Saturnispora were isolated from plant-related substrates, soil, and water samples.…”

“…Most species of Saturnispora were isolated from plant-related substrates, soil, and water samples. These yeasts are characterized by the fermentation and assimilation of only a few carbon compounds and are almost indistinguishable physiologically from one another (Kurtzman 2006;Boonmak et al 2009;Limtong et al 2010).…”

Candida asiatica sp. nov., an anamorphic ascomycetous yeast species isolated from natural samples from Thailand, Taiwan, and Japan

“…Species of this genus have been isolated from various sources in terrestrial and aquatic habitats. In recent years, many novel Candida species have been described from strains isolated in Thailand, Taiwan, and Japan from various natural samples, e.g., soils, estuarine waters, sediments in mangrove forests, insect frasses, and beetle galleries (Boonmak et al 2009;Endo et al 2008;Limtong et al 2007bLimtong et al , 2008Limtong et al , 2010Liu et al 2008;Nakase et al 2009). Phylogenetic analysis showed that these novel species are distributed in various clades, e.g., Ambrosiozyma, Metschnikowia, Pichia, Saturnispora, Wickerhamomyces, and Yarrowia.…”