<i>Chlamydia pneumoniae</i>Serology: Interlaboratory Variation in Microimmunofluorescence Assay Results

Peeling, Rosanna W.; Wang, S.-P.; Grayston, J. T.; Blasi, Francesco; Boman, Jens; Clad, Andreas; Freidank, Heike; Gaydos, Charlotte A.; Gnarpe, Judy; Hagiwara, Toshikatsu; Jones, Robert B.; Orfila, J; Persson, Kristina; Puolakkainen, Mirja; Saikku, Pekka; Schachter, Julius

doi:10.1086/315603

Cited by 121 publications

(112 citation statements)

References 12 publications

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“…Some validated commercial MIF kits [17,18] have been used by many clinical laboratories and researchers [11,17,[19][20][21]. Laboratorial diagnosis of C. pneumoniae infection in our study was established on the basis of a significant and clear-cut antibody response by MIF test as reported [11] and recommended [5]; we use seroconversion as criterium of definite acute infection as others [6].…”

Section: Discussionmentioning

confidence: 99%

Community-acquired pneumonia by Chlamydophila pneumoniae: a clinical and incidence study in Brazil

Chedid¹,

Chedid²,

Ilha³

et al. 2007

Braz J Infect Dis

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As there was not any data on Chlamydia pneumoniae (TWAR) infections in Brazil so far, a prospective cohort study of adult patients hospitalized due to CAP was carried out for one year in a Brazilian university general hospital to detect the incidence of CAP by Chlamydophila pneumoniae (TWAR) for one year. During a whole year 645 consecutive patients hospitalized due to an initial presumptive diagnosis of respiratory diseases by ICD-10 (J00-J99), excluding upper respiratory diseases, were screened; 59 consecutive patients with CAP were diagnosed. They had determinations of serum antibodies to C. pneumoniae by microimmunofluorescence at the Infectious Diseases Laboratory of University of Louisville (KY, USA); 37 patients (63.8%) had seroreactivity to TWAR antigens, from which 23 (39.6%) had previous infection; 3 patients (5.2%) were diagnosed with CAP by TWAR and got cured. The incidence of TWAR CAP in our hospital by seroconversion was 5.2%. Our incidence of 5.2% is probably underestimated since TWAR culture was not available; we suggest that Real-Time PCR be used along with other diagnostic methods in future studies to detect the actual incidence of TWAR CAP. We propose that the serological criterion of IgM ≥1:16 alone to the diagnosis of acute infection by TWAR are discontinued due a lack of specificity.

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Section: Discussionmentioning

confidence: 99%

Community-acquired pneumonia by Chlamydophila pneumoniae: a clinical and incidence study in Brazil

Chedid¹,

Chedid²,

Ilha³

et al. 2007

Braz J Infect Dis

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“…However, the MIF test is dif®cult to standardise, and is technically demanding. As a result, the use of this assay has been restricted to a relatively small number of quali®ed research laboratories [34]. The results presented in this paper suggest that a more widely applicable enzyme immunoassay, based upon recombinant MOMP polyantigens, may be helpful in the serodiagnosis of C. trachomatis infections.…”

Section: Discussionmentioning

confidence: 99%

Detection of Chlamydia trachomatis-specific antibodies in human sera by recombinant major outer-membrane protein polyantigens

Mygind¹,

Christiansen

Persson³

et al. 2000

Journal of Medical Microbiology

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This study was performed to generate and evaluate recombinant antigens for use in a species-speci®c Chlamydia trachomatis immunoassay. In a molecular genetic approach, fragments of the C. trachomatis major outer-membrane protein (MOMP) were produced as fusion proteins to create three different constructs encompassing the variable domains I, II and IV of selected C. trachomatis serovars. The recombinant MOMP polyantigens were af®nity-puri®ed and used in an enzyme-linked immunosorbent assay. Antibody detection was evaluated with 103 patient sera and the results were compared with titres obtained in the micro-immuno¯uorescence test. The results showed that the generated MOMP polyantigens detected the presence of C. trachomatis-speci®c human antibodies with little cross-reaction to C. pneumoniae-speci®c antibodies. When compared to the micro-immuno¯uorescence assay the MOMP polyantigen detected the presence of anti-C. trachomatis IgG antibodies with a sensitivity of 80% and a speci®city of 91%.

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“…However, MIF is not ideal for routine serodiagnostics because it is labour intensive, highly observer dependent, and interlaboratory variation is significant [13]. Enzyme immunoassays (EIA) based on synthetic peptides characterized by high throughput, objective endpoints, and technical accessibility are commercially available [14], but are generally considered to be inferior to MIF in predicting tubal factor subfertility [15].…”

Section: Introductionmentioning

confidence: 99%

Comparison of four serological assays for the diagnosis of Chlamydia trachomatis in subfertile women

Muvunyi

Claeys

Sutter

et al. 2011

J Infect Dev Ctries

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Introduction: Chlamydia antibody testing (CAT) in serum has been introduced as a screening method in the infertility workup. We evaluated the test characteristics of two ELISA tests compared to micro-immunofluorescence tests (MIFs). MIFs are considered the gold standard in the C. trachomatis IgG antibodies detection. We also compared the accuracy of all CAT tests in predicting tubal subfertility, using laparoscopy as a reference. Methodology: Four commercial serological methods were used to analyse 101 serum samples for the presence of C. trachomatis IgG antibodies from patients at the Infertility Clinic of Ghent University Hospital. The diagnostic utility for prediction of tubal infertility of serological methods was evaluated based on patients' medical records. Results: A comparison of the serological assays showed little difference in the major performance characteristics: the sensitivities of all MIFs and ELISAs were 100% for all assays (except the ELISA Vircell, with a sensitivity of 90%), and the specificities ranged from 92% for MIF Ani Labsystems to 98% for the MIF Focus and ELISA Vircell. As compared to laparoscopy data, CAT positivity in subfertile women with tubal damage (n=40) did not significantly differ from that of subfertile women without tubal damage (n=61): Positive predictive values (PPV) of CAT ranged from 53% to 60% and negative predictive values (NPV) ranged from 62% to 64%. Conclusion: evaluated ELISAs are comparable to MIFs in the detection of C. trachomatis IgG antibodies and should be preferred for large serological studies, especially in resource poor settings.

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Chlamydia pneumoniaeSerology: Interlaboratory Variation in Microimmunofluorescence Assay Results

Cited by 121 publications

References 12 publications

Community-acquired pneumonia by Chlamydophila pneumoniae: a clinical and incidence study in Brazil

Community-acquired pneumonia by Chlamydophila pneumoniae: a clinical and incidence study in Brazil

Detection of Chlamydia trachomatis-specific antibodies in human sera by recombinant major outer-membrane protein polyantigens

Comparison of four serological assays for the diagnosis of Chlamydia trachomatis in subfertile women

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