Coronostoma claireaen. sp. (Nematoda: Rhabditida: Oxyuridomorpha: Coronostomatidae) from the Indigenous MillipedNarceus gordanus(Chamberlain, 1943) (Diplopoda: Spirobolida) in Ocala National Forest, Florida

Abstract

Twenty-four individuals of Narceus gordanus (Diplopoda: Spirobolidae) were collected in Ocala National Forest, FL, between November 2013 and July 2014. Each specimen was dissected to extract the intestine, which was removed and examined for parasitic nematodes. Coronostoma claireae n. sp. was collected from the hindgut and midgut of 10 specimens, and its morphology was examined with brightfield, differential interference contrast, phase contrast, and scanning electron microscopy. This species is separated from other Coronostoma spp. by the following characteristics: body length less than 3 mm; head sense organs pit-like; first annule long, extending past middle of corpus, width similar to that of second annule; basal bulb pyriform; eggs larger than 60 3 50 mm. This species is the first North American record for the genus Coronostoma, which is removed from Thelastomatoidea: Thelastomatidae and reassigned to Coronostomatidae on the basis of presumed apomorphies. A key is provided for known Coronostoma spp. The superfamily Coronostomatoidea is re-established for Coronostomatidae and Traklosiidae.

A new species ofTravassosinema(Oxyuridomorpha: Travassosinematidae) from an Indian millipede,Trigoniulus corallinus(Gervais)

“…Millipedes were dissected by severing the head and epiproct with a razor blade as described by Phillips et al (2016). The whole gut was pulled out intact from the body cavity with a fine tip forcep and put in a watch glass containing saline water.…”

Stauratostoma shelleyi n. gen., n. sp. (Nematoda: Rhabditida: Thelastomatidae) from Appalachian Polydesmid Millipedes (Polydesmida: Xystodesmidae)

“…Millipedes were dissected by severing the head and epiproct with a razor blade as described by Phillips et al (2016). The intestine was pulled intact from the body cavity with fine-tip forceps and placed in a Syracuse watch glass containing distilled water.…”

“…For SEM, methods used by Phillips et al (2016) were followed. Formalin-fixed nematodes were washed in distilled water for 20 min then placed into a 12-mm × 30-µm microporous specimen capsule (Electron Microscopy Services, Hatfield, PA).…”

Two species of Thoracostomopsidae (Nematoda: Enoplida) from Jeju Island, South Korea

“…Drops of distilled water were added gradually to the drop of glycerin to rehydrate the specimen. Hydrated specimens underwent ethanol series for dehydration (20%, 40%, 50%, 70%, 80%, 90%, 95%, 100%, for 10 min each) to be placed in hexamethyldisilazane (HMDS), with slightly altered concentration and duration compared to a method used by Phillips et al (2016). A pool of HMDS containing the specimen was placed in drying oven to be completely dried overnight.…”