<i>Cryptosporidium</i> and <i>Giardia</i> as water contaminant pathogens in Hungary

Plutzer, Judit

doi:10.1556/oh.2013.29749

Cited by 3 publications

(2 citation statements)

References 154 publications

(211 reference statements)

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“…Waterborne parasitic protozoan diseases with worldwide distribution, result in four billion cases of diarrhoea, 1.6 million deaths annually (www.who.int) and 62.5 million Disability Adjusted Life Years (DALYs) worldwide (Wright and Gundry, 2009, WHO Guidelines for Drinking Water Quality., 2011). Yet, despite the latest advances made in water treatment measures, protecting drinking water supplies against waterborne pathogens remains by far, as one of the most challenging concerns for the entire drinking water supply chain worldwide (Cotruva et al., 2004, Betancourt and Rose, 2004, Thompson and Smith, 2011, Plutzer, 2013, Burnet et al., 2014). In response to this, in 2009, the World Health Organization has developed guidelines for water suppliers on how to implement “Water Safety Plans” (WSPs), in the hope of halving the number of people without safe access to drinking water by the end of 2015 (WHO, 2009).…”

Section: Introductionmentioning

confidence: 99%

Public health significance of zoonotic Cryptosporidium species in wildlife: Critical insights into better drinking water management

Zahedi

Paparini

Jian

et al. 2016

International Journal for Parasitology: Parasites and Wildlife

162

144

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Cryptosporidium is an enteric parasite that is transmitted via the faecal–oral route, water and food. Humans, wildlife and domestic livestock all potentially contribute Cryptosporidium to surface waters. Human encroachment into natural ecosystems has led to an increase in interactions between humans, domestic animals and wildlife populations. Increasing numbers of zoonotic diseases and spill over/back of zoonotic pathogens is a consequence of this anthropogenic disturbance. Drinking water catchments and water reservoir areas have been at the front line of this conflict as they can be easily contaminated by zoonotic waterborne pathogens. Therefore, the epidemiology of zoonotic species of Cryptosporidium in free-ranging and captive wildlife is of increasing importance. This review focuses on zoonotic Cryptosporidium species reported in global wildlife populations to date, and highlights their significance for public health and the water industry.

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Section: Introductionmentioning

confidence: 99%

Public health significance of zoonotic Cryptosporidium species in wildlife: Critical insights into better drinking water management

Zahedi

Paparini

Jian

et al. 2016

International Journal for Parasitology: Parasites and Wildlife

162

144

View full text Add to dashboard Cite

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“…DNAs were extracted from all stool samples that were purified with sucrose gradient method, with the QIAamp DNA Mini Kit using the protocol modified by Plutzer et al 13 Hot Start Taq DNA Polymerase Kit (Qiagen), 25 mM dNTP mix, 10 pmol CPBD AGF, and CPBDIAGR primers;…”

Section: Dna Isolation and Imaging Of Cryptosporidium Oocysts With Pcr Methodsmentioning

confidence: 99%

Investigation of Cryptosporidium spp. Positivity in Formol-Fixed Stool Samples by Polymerase Chain Reaction Method

Karaman¹,

Kolören²,

Ayaz³

2019

Cumhuriyet Medical Journal

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It was aimed to determine Cryptosporidium spp., which is thought to cause digestive system complaints in primary school children, by Modified Acid-Fast (MAF) staining method and Polymerase Chain Reaction (PCR). Method: One hundred sixty-four stool samples taken from children were examined under light microscopy with MAF. Positive samples were fixed in 10% formalin and stored at + 4 °C until analysis. These samples were then purified using the Sucrose Gradient method and, DNA isolation was performed using the modified QIAamp DNA Mini Kit (Qiagen) protocol. In order to increase the sensitivity of the study, the SSU rRNA gene region was amplified by PCR method. Results: Microscopic examination showed that 89 of the 164 stool samples (54,26%) were found to be positive for Cryptosporidium spp.oocysts. Eightynine samples which were found to be positive by microscopic examination and fixed in %10 formalin were studied by PCR, and 74 (45,12%) of them were positive. Conclusions: Cryptosporidiosis, which is known to cause severe infections, especially in immunocompromised individuals and children, is transmitted by the fecal-oral route from infected humans and animals, and contaminated water and nutrients. Cryptosporidiosis, which is the result of fecal pollution, causes a great risk for human health even with a low number of oocysts due to the fact that oocysts are resistant to chlorine and they can survive for a long time in the outer environment. It was observed that primary school children living in the area of the study were under risk for cryptosporidiosis.

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Occurrence of Cryptosporidium in the water basins of nitra region, slovakia

et al. 2018

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Cryptosporidium and Giardia as water contaminant pathogens in Hungary

Abstract: The presented results contribute to a better understanding the epidemiology and relevance of waterborne parasites, their surveillance and performance of future control measures to prevent waterborne infections in Hungary.

Cited by 3 publications

References 154 publications

Public health significance of zoonotic Cryptosporidium species in wildlife: Critical insights into better drinking water management

Public health significance of zoonotic Cryptosporidium species in wildlife: Critical insights into better drinking water management

Investigation of Cryptosporidium spp. Positivity in Formol-Fixed Stool Samples by Polymerase Chain Reaction Method

Occurrence of Cryptosporidium in the water basins of nitra region, slovakia

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