<i>Cryptosporidium</i>
            Genotypes in Wildlife from a New York Watershed

Feng, Yaoyu; Alderisio, Kerri A.; Yang, Wenli; Blancero, Lisa A.; Kuhne, William G.; Nadareski, Christopher A.; Reid, Michael J. C.; Xiao, Lihua

doi:10.1128/aem.01034-07

Cited by 145 publications

(167 citation statements)

References 27 publications

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“…Isolate W971 was homologous with genotype W13 found in storm water, which, in turn, is the skunk genotype (6). Initially, the BLAST search for isolate W6863 erroneously indicated C. parvum as the most probable identity at the SSU rRNA gene, but this was due to the short length (484 bp) of the only horse genotype sequence available (AY273770) for comparison.…”

Section: The Studymentioning

confidence: 99%

UnusualCryptosporidiumGenotypes in Human Cases of Diarrhea

Robinson¹,

Elwin²,

Chalmers³

2008

Emerg. Infect. Dis.

118

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Section: The Studymentioning

confidence: 99%

UnusualCryptosporidiumGenotypes in Human Cases of Diarrhea

Robinson¹,

Elwin²,

Chalmers³

2008

Emerg. Infect. Dis.

118

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“…New York, USA, have been given temporary sequential genotype numbers prefixed by the letter W (water) until a host is identified (Feng et al 2007). …”

Section: Colleagues In Watersheds Inmentioning

confidence: 99%

“…UK E4 was most similar to but distinct from the fox genotype, and was found in a stream beside property 7; UK E6 was most similar to but distinct from Cryptosporidium canis and was Figure 1 | Phylogenetic relationships among Cryptosporidium isolates from this study and known species and genotypes reported on GenBank, as inferred by neighbour-joining analysis. Water genotype names are given in brackets after their animal hosts, according to Feng et al 2007. Numbers on branch nodes are bootstrap values (.50%) obtained from 1000 re-samples.…”

Section: Cryptosporidium Speciesmentioning

confidence: 99%

“…This has enabled the identification of the species and genotypes present and provides additional information about possible sources of contamination and the risk to public health. However, when source and storm waters were sampled, only some of the isolates detected matched named Cryptosporidium species or genotypes, while others were also found in animals in the catchment thus generating a common name for the genotype (Feng et al 2007). Isolates found only in water by Feng and …”

Section: Introductionmentioning

confidence: 97%

“…Most other Cryptosporidium species and genotypes cause human disease doi: 10.2166/wh.2009.185 more rarely (Chalmers et al 2009b). Many exhibit some degree of host specificity, providing some indication of potential source when detected in water (Feng et al 2007). Notable exceptions appear to include the cervine genotype which has been found in a variety of hosts (Santin & Fayer 2007).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Detection of Cryptosporidium species and sources of contamination with Cryptosporidium hominis during a waterborne outbreak in north west Wales

Chalmers

Robinson

Elwin

et al. 2009

Journal of Water and Health

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As part of investigations into the cause of a waterborne outbreak of Cryptosporidium hominis infection linked to a mains water supply, surface waters and wastewater treatment plants were tested for Cryptosporidium spp. Oocyst counts in base flow surface water samples ranged from nil to 29 per 10 l. Oocyst counts in effluent from a community wastewater treatment plant were up to 63 fold higher and breakout from one septic tank five logs higher. There were no peak (storm) flow events during the investigation. C. hominis, four named genotypes (cervine, muskrat II, rat, W19) and six new small subunit ribosomal RNA gene sequences were identified. Four of the new sequences were closely related to Cryptosporidium muskrat genotype I, one was closely related to the fox genotype and one to Cryptosporidium canis. C. hominis was found extensively in the catchment, but only at sites contaminated by wastewater, and in the treated water supply to the affected area. All were gp60 subtype IbA10G2, the outbreak subtype.Multiple routes of contamination of the reservoir were identified, resulting in persistent detection of low numbers of oocysts in the final water. This work demonstrates the utility of genotyping Cryptosporidium isolates in environmental samples during outbreak investigations.

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Molecular evaluation of Cryptosporidium spp. among breeding calves of Lorestan province Western Iran

Talebi

Mamaghani

Kheirandish

et al. 2022

Veterinary Medicine & Sci

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Background Cryptosporidium spp. are opportunistic intestinal protozoans with global distribution and are of great importance as zoonotic protozoans are common to humans and domestic animals, including cattle and calves. Identification and detection of parasite species using precise methods including molecular methods can be an effective step in treating and controlling parasites. Objectives This study aimed to investigate the prevalence of Cryptosporidium among breeding calves of Khorramabad city, Lorestan province, Western Iran, using PCR. Methods The faecal samples were taken from 181 healthy and diarrhoeal calves and after the Ziehl Neelsen Acid‐fast staining and microscopic evaluation, the genomic DNA was extracted for molecular evaluations. To detect Cryptosporidium species, specific primers targeting the SAM‐1 gene of Cryptosporidium and a commercial master mix were used for PCR. Results Out of 181 faecal samples of breeding calves in Khorramabad city, 9 samples (5%) were positive for Cryptosporidium spp. using the PCR method. Statistical analysis of the data showed that there was no significant statistical relationship between Cryptosporidium infection of the calves and variables of age, breed, type of water consumption, clinical signs of diarrhoea, and sampling location, while parasite infection had a significant relationship with calf gender so that all Cryptosporidium positive samples were from male calves ( p ≤ 0.05). Conclusions Considering the presence of Cryptosporidium infection, the region's traditional grazing system, and the close relationship between livestock and humans, there is a possibility of human infection in the region. So preventive measures such as periodic animal testing with sensitive and accurate diagnostic techniques including PCR, pharmacological treatment of livestock, water hygiene and the use of industrial grazing instead of traditional grazing to improve the hygiene of food consumed by livestock are recommended.

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Cryptosporidium Genotypes in Wildlife from a New York Watershed

Cited by 145 publications

References 27 publications

UnusualCryptosporidiumGenotypes in Human Cases of Diarrhea

UnusualCryptosporidiumGenotypes in Human Cases of Diarrhea

Detection of Cryptosporidium species and sources of contamination with Cryptosporidium hominis during a waterborne outbreak in north west Wales

Molecular evaluation of Cryptosporidium spp. among breeding calves of Lorestan province Western Iran

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