<i>De Novo</i>Sequencing and Characterization of the Transcriptome of Dwarf Polish Wheat (<i>Triticum polonicum</i>L.)

Wang, Yi; Wang, Chao; Wang, Xiaolu; Peng, Fan; Wang, Ruijiao; Jiang, Yulin; Zeng, Jian; Fan, Xing; Kang, Houyang; Li, Sha; Zhang, Haiqin; Xiao, Xue; Zhou, Yong‐Hong

doi:10.1155/2016/5781412

Cited by 8 publications

(6 citation statements)

References 34 publications

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“…While mining the public transcriptome database for the discovery of novel viral sequences [ 32 ], we discovered the presence of an unannotated TSA sequence related to WhCV1 (accession no. GEDL01038361, 15,366 nt in length) in the transcriptomic data of Polish wheat ( Triticum polonicum L.) from a Chinese research group [ 33 ]. During the preparation and submission of this paper, another research group mined the same TSA sequence for the same virus as we discovered and tentatively named it Triticum polonicum closterovirus (TriPCV; TPA no.…”

Section: Resultsmentioning

confidence: 99%

Discovery and Genome Characterization of a Closterovirus from Wheat Plants with Yellowing Leaf Symptoms in Japan

et al. 2023

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Many aphid-borne viruses are important pathogens that affect wheat crops worldwide. An aphid-transmitted closterovirus named wheat yellow leaf virus (WYLV) was found to have infected wheat plants in Japan in the 1970s; however, since then, its viral genome sequence and occurrence in the field have not been investigated. We observed yellowing leaves in the 2018/2019 winter wheat-growing season in an experimental field in Japan where WYLV was detected five decades ago. A virome analysis of those yellow leaf samples lead to the discovery of a closterovirus together with a luteovirus (barley yellow dwarf virus PAV variant IIIa). The complete genomic sequence of this closterovirus, named wheat closterovirus 1 isolate WL19a (WhCV1-WL19a), consisted of 15,452 nucleotides harboring nine open reading frames. Additionally, we identified another WhCV1 isolate, WL20, in a wheat sample from the winter wheat-growing season of 2019/2020. A transmission test indicated that WhCV1-WL20 was able to form typical filamentous particles and transmissible by oat bird-cherry aphid (Rhopalosiphum pad). Sequence and phylogenetic analyses showed that WhCV1 was distantly related to members of the genus Closterovirus (family Closteroviridae), suggesting that the virus represents a novel species in the genus. Furthermore, the characterization of WhCV1-WL19a-derived small RNAs using high-throughput sequencing revealed highly abundant 22-nt-class small RNAs potentially derived from the 3′-terminal end of the WhCV1 negative-strand genomic RNA, indicating that this terminal end of the WhCV1 genome is likely particularly targeted for the synthesis of viral small RNAs in wheat plants. Our results provide further knowledge on closterovirus diversity and pathogenicity and suggest that the impact of WhCV1 on wheat production warrants further investigations.

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Section: Resultsmentioning

confidence: 99%

Discovery and Genome Characterization of a Closterovirus from Wheat Plants with Yellowing Leaf Symptoms in Japan

et al. 2023

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“…PCR primers (F: 5′-TCATTGGGAGAGAGTGAGCAT-3′; R: 5′-ACATATCTAGTTT CCTCGCTGC-3′) for the full-length cDNA of TpNRAMP3 were designed according to DPW transcript ( Wang et al, 2016 ). PCR volume included 2.5 μl 10× buffer, 2.0 μl MgCl 2 (25 mM), 2.0 μl dNTP mixture (2.5 Mm each), 2.0 μl forward primer (4 pM), 2.0 μl reverse primer (4 pM), 3.0 μl cDNA, 0.5 μl ExTaq, and added sterilized distilled water up to 25 μl.…”

Section: Methodsmentioning

confidence: 99%

Cloning and Characterization of TpNRAMP3, a Metal Transporter From Polish Wheat (Triticum polonicum L.)

et al. 2018

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Essential transition metals and non-essential metals often co-exist in arable soils. In plants, some transition metal transporters, such as the natural resistance-associated macrophage proteins (NRAMPs), poorly selectively transport metals with similar chemical properties whether they are essential or non-essential. In this study, a member of the NRAMP transporter family, TpNRAMP3, was identified from dwarf Polish wheat (Triticum polonicum L.). TpNRAMP3 encodes a plasma membrane-localized protein and was highly expressed in leaf blades and roots at the jointing and booting stages, and in the first nodes at the grain filling stage. Expression of TpNRAMP3 increased sensitivity to Cd and Co, but not Zn, and increased the Cd and Co concentrations in yeast. TpNRAMP3 expression in Arabidopsis increased concentrations of Cd, Co, and Mn, but not Fe or Zn, in roots, shoots, and whole plant. However, TpNRAMP3 did not affect translocation of Cd, Co, or Mn from roots to shoots. These results suggest that TpNRAMP3 is a transporter for Cd, Co, and Mn accumulation, but not for Fe or Zn. However, Cd and Co are non-essential toxic metals; selective genetic manipulation of TpNRAMP3 will help breed low Cd- and Co-accumulating cultivars.

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“…The full-length cDNAs of TpIRT1A and TpIRT1B were ampli ed from leaves. PCR primers were selected based on the reference sequence of the wheat genome (Wang et al 2016) were designed using Beacon Designer v7.0 (PREMIER Biosoft International, California, USA) (Table S1). Ten tetraploid wheat and two diploid ancestral species were used to investigate IRT1 homoeologs with the same primers (Table S2).…”

Section: Gene Cloning Bioinformatics and Phylogenetic Analysismentioning

confidence: 99%

“…Here, we thought to characterize IRT1 homoeologous from wheat. We speci cally focused on Dwarf Polish Wheat (DPW) because it can accumulate high concentrations of Cd, Zn, and Fe in its seedlings without showing toxicity symptoms (Wang et al 2016). DPW (Triticum polonicum L., 2n = 4x = 28, AABB) was originally collected from Tulufan, Xinjiang province, China, by Prof. Chi Yen of the Sichuan Agricultural University, China.…”

Section: Introductionmentioning

confidence: 99%

TpIRT1 is a transition metal transporter in Polish Wheat (Triticum polonicum L.) with a broad substrate specificity

Jiang¹,

Chen²,

Chai³

et al. 2021

Preprint

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Aims (1) Explore the metal substrate specificity of homologous TpIRT1A and TpIRT1B transporters from dwarf Polish wheat by expressing them in protoplast, yeast, and transgenic Arabidopsis; (2) screen polymorphic residues of IRT1 homologs from tetraploid and diploid ancestral wheat species that change the substrate specificity.Methods Two IRT1 homoeologs were isolated from A (TpIRT1A) and B (TpIRT1B) genomes of a tetraploid crop, polish wheat (Triticum polonicum). Both of them were analysed by expressing them in yeast and Arabidopsis protoplast, respectively. Then we constructed over-expressing transgenic plants of TpIRT1B for metals property analysis in Arabidopsis. We also isolated 22 IRT1 homoeologs from tetraploid and diploid ancestral wheat species and expressed them in yeast for function analysis. Results Our data highlighted the importance of TpIRT1 in the uptake and translocation of Fe, Mn, Co, and Cd with direct implications for wheat yield potential. Both TpIRT1A and TpIRT1B were located at the plasma membrane and internal vesicles in Arabidopsis protoplasts, and responsible for Cd and Co sensitivity in yeast. The over-expression of TpIRT1B in A. thaliana increased Fe, Mn, Co, and Cd concentration in its tissues and improved plant growth under Fe, Mn, and Co deficiencies, while causing more sensitivity to Cd than wild-type plant. Functional analysis of IRT1 homoeologs from tetraploid and diploid ancestral wheat species in yeast disclosed four distinct amino acid residues in TdiIRT1B (T. dicoccum) and TtuIRT1B (T. turgidum). Altogether, these results increase the knowledge of IRT1 function in a global crop, wheat.

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De NovoSequencing and Characterization of the Transcriptome of Dwarf Polish Wheat (Triticum polonicumL.)

Cited by 8 publications

References 34 publications

Discovery and Genome Characterization of a Closterovirus from Wheat Plants with Yellowing Leaf Symptoms in Japan

Discovery and Genome Characterization of a Closterovirus from Wheat Plants with Yellowing Leaf Symptoms in Japan

Cloning and Characterization of TpNRAMP3, a Metal Transporter From Polish Wheat (Triticum polonicum L.)

TpIRT1 is a transition metal transporter in Polish Wheat (Triticum polonicum L.) with a broad substrate specificity

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