Detarium microcarpum, Guiera senegalensis, and Cassia siameaInduce Apoptosis and Cell Cycle Arrest and Inhibit Metastasis on MCF7 Breast Cancer Cells

Abstract

Despite the availability of anticancer drugs, breast cancer remains the most death-causing tumor-related disease in women. Hence, there is a need for discovery and development of efficient alternative drugs, and sources such as plants need to be explored. In this study, antioxidant capacities and inhibitory effects against MCF7 cells of the extracts of stem bark of three Nigerian medicinal plants (Detarium microcarpum, Guiera senegalensis, and Cassia siamea) were investigated. The D. microcarpum extracts had the highest antioxidant and antiproliferative effects, followed by that of G. senegalensis, and the C. siamea extracts had minimal effects. The IC50 values of the methanol and aqueous extracts from the three plants that inhibited the proliferation of MCF7 cells ranged from 78–> 500 μg/ml. Moreover, all the plant extracts but the aqueous extract of Cassia siamea exhibited antimetastatic action and induced apoptosis and cell cycle arrest in MCF7 cells. Liquid chromatography/time-of-flight/mass spectrometry profiling revealed that the five potent extracts contain many phenols and omega-6 fatty acids, and some of the identified compounds (isorhamnetin, eupatorin, alpinumisoflavone, procyanidin B3, syringin, and gallic acid) have been reported to have antiproliferative effects on cancer cells. Hence, the stem bark of these plants could be potential sources of antibreast cancer agents.

Boswellia dalzielii-Mediated Silver Nanoparticles Inhibited Acute Myeloid Leukemia (AML) Kasumi-1 Cells by Inducing Cell Cycle Arrest

“…One milligram of the synthesized nanoparticles quenched 53.73% of the oxidant DPPH solution, and its TEAC value was calculated to be 300.91 ± 13.42 μ M Trolox/mg silver nanoparticles. The amount is considered significant when compared with the antioxidant capacities of other nanoparticles and medicinal plants [ 21 , 35 – 37 ]. El-Rafie and Hamed [ 38 ] reported that Terminalia catappa extract-mediated silver nanoparticles had significant free radical scavenging effect and high antioxidant activity.…”

“…The DPPH assay was carried out using a modification of the procedure described by [ 21 ]. Briefly, 150 μ l of 0.2 mM DPPH solution (Sigma-Aldrich, Darmstadt, Germany) was added to either 50 μ l of the synthesized silver nanoparticles or 50 μ l of varying concentrations (0, 30, 60, 120, 240, and 480 μ g/ml) of Trolox antioxidant solution (Acros Organics, Newton Dr, Carlsbad, CA, USA) in wells of a 96-well plate.…”

“…The inhibitory effect of the synthesized silver nanoparticles on AML Kasumi-1 cells was investigated using PrestoBlue antiproliferative assay according to the experimental procedure described by [ 21 ]. The cells (5000 cells/well) were seeded into the wells of a 96-well plate and treated with different concentrations of the silver nanoparticles (0–70 μ g/ml) for 48 and 72 hours.…”

Inhibitory Effect of Eco-Friendly Naturally Synthesized Silver Nanoparticles from the Leaf Extract of Medicinal Detarium microcarpum Plant on Pancreatic and Cervical Cancer Cells

“…The plant is highly rich in electron donating phytochemical compounds such as flavonoids, polyphenols, diterpenes and polysaccharides (Labulo et al, 2016). It is also reported that D. microcarpum has strong antioxidant capacity to scavenge free radicals such as intracellular ROS of cancer cells (Adebayo et al, 2019;Lamien-Meda et al, 2008;Rouamba et al, 2017). In this study, we synthesized silver nanoparticles using the extract of Detarium microcarpumleaves and the cytotoxic effects of the synthesized nanoparticles (dAgNPs) on pancreatic (PANC-1) and cervical (HeLa) cancer cells were evaluated for the first time.…”

Revue bibliographique sur la composition chimique et les activités biologiques de <i>Guiera senegalensis</i> J.F. Gmel. (Combretaceae)

“…Récemment, l'effet inhibiteur vis-à-vis des cellules cancéreueses MCF7 d'extrait méthanolique d'écorces de tige de G. senegalensis a été déterminé à l'aide du test de viabilité cellulaire Presto Blue. Il avait un effet antiprolifératif avec une CI 50 de 141 μg/mL et en outre, il présentait une action antimétastatique et induisait une apoptose (CI 50 de 29,8%) et un arrêt du cycle cellulaire dans les cellules MCF7 (Adebayo et al, 2019).…”