1983
DOI: 10.1111/j.1570-7458.1983.tb03301.x
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Development of artificial diets for laboratory rearing of larvae of the spiny bollworm, Earias insulana

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1983
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Cited by 10 publications
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“…E. Vitella. For rearing and bioassay, modified Klein medium was used, replacing alfalfa meal with bhendi [Abelmoschus esculentus (L) Moench] seed powder (8). The diet contained 700 mL of sterile water, 15 g of agar, 30 g of yeast, 100 g of bhendi seed meal, 2 g of sorbic acid, 2 g of choline chloride, 4 g of ascorbic acid, 2.5 g of p-hydroxybenzoic acid methyl ester, 7 g of Wesson's salt mixture, 2 mL of vitamins (ABDEC), 2.5 mL of formaldehyde, and 0.1 unit of streptomycin.…”
Section: Methodsmentioning
confidence: 99%
“…E. Vitella. For rearing and bioassay, modified Klein medium was used, replacing alfalfa meal with bhendi [Abelmoschus esculentus (L) Moench] seed powder (8). The diet contained 700 mL of sterile water, 15 g of agar, 30 g of yeast, 100 g of bhendi seed meal, 2 g of sorbic acid, 2 g of choline chloride, 4 g of ascorbic acid, 2.5 g of p-hydroxybenzoic acid methyl ester, 7 g of Wesson's salt mixture, 2 mL of vitamins (ABDEC), 2.5 mL of formaldehyde, and 0.1 unit of streptomycin.…”
Section: Methodsmentioning
confidence: 99%
“…Rearing technique. The conditions for maintaining a mass culture o f E. insulana, and the composition and preparation o f the artificial diet were described previously (Klein et al, 1981(Klein et al, , 1983. Between 30 and 40 pupae, 3 -6 days old, were placed in three 500-ml glass jars and 10~ aqueous sucrose was added just before emergence o f the adults.…”
Section: Methodsmentioning
confidence: 99%