DICER-LIKE 1 and DICER-LIKE 3 Redundantly Act to Promote Flowering via Repression of FLOWERING LOCUS C in Arabidopsis thaliana

Schmitz, Robert J.; Hong, Lewis Z.; Fitzpatrick, Kathleen E.; Amasino, Richard M.

doi:10.1534/genetics.107.070649

Cited by 62 publications

(43 citation statements)

References 31 publications

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“…Comparative transcriptome analysis of dcl1 and dcl2 dcl3 dcl4 mutants supports the conclusion that DCL1 acts mainly in miRNA processing and that it fulfills this role largely independently of other DCL proteins. However, the four DCLs also have overlapping functions; for instance, they are all involved in RNA silencing of viral transcripts, and DCL1 and DCL3 act redundantly in the control of FLOWERING LOCUS C expression (29,41,76). To this, we can now add coordinated action of DCLs in silencing a subset of transposons, exemplified by AT1TE36060.…”

Section: Discussionmentioning

confidence: 99%

Global effects of the small RNA biogenesis machinery on the Arabidopsis thaliana transcriptome

Laubinger

Zeller

Henz

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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In Arabidopsis thaliana, four different dicer-like (DCL) proteins have distinct but partially overlapping functions in the biogenesis of microRNAs (miRNAs) and siRNAs from longer, noncoding precursor RNAs. To analyze the impact of different components of the small RNA biogenesis machinery on the transcriptome, we subjected dcl and other mutants impaired in small RNA biogenesis to whole-genome tiling array analysis. We compared both protein-coding genes and noncoding transcripts, including most primiRNAs, in two tissues and several stress conditions. Our analysis revealed a surprising number of common targets in dcl1 and dcl2 dcl3 dcl4 triple mutants. Furthermore, our results suggest that the DCL1 is not only involved in miRNA action but also contributes to silencing of a subset of transposons, apparently through an effect on DNA methylation.ike other plants, the widely used model species Arabidopsis thaliana produces a complex population of small RNAs (sRNAs). These sRNAs come in two major flavors: microRNAs (miRNAs), most of which are 20-22 nt long, and siRNAs, with a typical length of 23-24 nt. Most sRNAs are derived from longer precursor RNAs, which are either dsRNA molecules or ssRNA molecules that form a self-complementary fold-back structure. These RNAs are processed to sRNAs by four different dicer-like (DCL) proteins, DCL1, DCL2, DCL3, and DCL4 (reviewed in ref. 1).DCL1 is mainly involved in the generation of miRNAs, which are derived from longer primary miRNA (pri-miRNA) transcripts that are transcribed by polymerase II (polII) (2). PrimiRNAs are first trimmed by DCL1 to precursor miRNAs (pre-miRNAs), from which DCL1 further excises the miRNA/ miRNA* duplexes (3). DCL1 interacts with the dsRNA binding protein hyponastic leaves 1 (HYL1) and the zinc-finger protein serrate (SE) (4-10). Formation of this complex occurs in nuclear dicing bodies and is required for accurate processing activity of DCL1 (10, 11). The core miRNA biogenesis machinery probably acts in concert with associated factors that ensure proper processing of pri-miRNAs. These include the forkhead-associated domain containing protein dawdle (DDL) and the components of the nuclear cap binding complex abscisic acid ABA hypersensitive 1 (ABH1)/Cap-Binding Protein (CBP) . Processed miRNAs subsequently associate with one of the ten Arabidopsis argonaute (AGO) proteins to regulate their target mRNAs by transcript cleavage and/or inhibition of translation (16-22) until the miRNA is degraded by specific sRNA degradation nuclease (SDN) proteins (23).Other classes of sRNAs are mainly produced by DCL2, DCL3, and DCL4. SiRNAs derived from natural antisense transcripts (nat-siRNAs) are generated by DCL1 and DCL2 (24). DCL4 mainly acts in the biogenesis of transacting siRNAs (tasiRNAs) and in the generation of mobile siRNAs that communicate silencing effects between cells, but DCL4 also generates miRNAs from almost perfectly complementary miRNA fold backs (25-29). DCL3 acts in concert with RNA-dependent RNA polymerase 2 (RDR2) to generate heterochromatic...

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Section: Discussionmentioning

confidence: 99%

Global effects of the small RNA biogenesis machinery on the Arabidopsis thaliana transcriptome

Laubinger

Zeller

Henz

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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“…Although there is evidence that small RNAs can contribute to the regulation of FLC under non-vernalized conditions, small RNA components appear to be dispensable for vernalization-mediated FLC repression. 15,16 Unlike many PRC2-mediated repressions during development in eukaryotes, vernalization triggers PRC2-mediated repression with slow kinetics; it takes several weeks of cold exposure. By taking advantage of the slow kinetics of vernalization response, differential expression of transcripts could be monitored.…”

Section: The Role Of Coldair In Vernalizationmentioning

confidence: 99%

Encoding memory of winter by noncoding RNAs

Heo

Sung

2011

Epigenetics

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“…Association of COLDAIR triggers PRC2 targeting to FLC, a situation that leads to FLC repression during vernalization (Swiezewski et al 2009;Heo and Sung 2011). Expression of FLC is partially controlled by miRNAs since mutations within the miRNA biogenesis genes DICER-LIkE 1 ( DCL1) and DICER-LIkE 3 ( DCL3) can lead to delayed flowering due to excessively high expression of FLC in these mutant backgrounds (Schmitz et al 2007). Thus, long non-coding and miRNAs both play roles in the vernalization-mediated flowering.…”

Section: Vernalization/chilling-mediated Gene Repression or Activatiomentioning

confidence: 99%

Dormancy Behaviors and Underlying Regulatory Mechanisms: From Perspective of Pathways to Epigenetic Regulation

Liu

Zhu

Abbott

2015

Advances in Plant Dormancy

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DICER-LIKE 1 and DICER-LIKE 3 Redundantly Act to Promote Flowering via Repression of FLOWERING LOCUS C in Arabidopsis thaliana

Cited by 62 publications

References 31 publications

Global effects of the small RNA biogenesis machinery on the Arabidopsis thaliana transcriptome

Global effects of the small RNA biogenesis machinery on the Arabidopsis thaliana transcriptome

Encoding memory of winter by noncoding RNAs

Dormancy Behaviors and Underlying Regulatory Mechanisms: From Perspective of Pathways to Epigenetic Regulation

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