<i>Drosophila</i>female-specific brain neuron elicits persistent position- and direction-selective male-like social behaviors

Wu, Yang; Ss, Bidaye; Mahringer, David

doi:10.1101/594960

Cited by 8 publications

(20 citation statements)

References 41 publications

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“…Two types have a ring dorsal to the lateral junction ('pC1-Las-a/b'; Las for Lasso; similar to pC1a,b in Wang et al 2020), two have a long medial projection ('pC1-Med-a,b'; Med for Medial; pC1-Med-a is similar to pC1c in Wang et al 2020), one has dense processes mediodorsal to the lateral junction (pC1e; same as in Wang et al 2020). Two other pC1 types have a horizontal projection medial to the 'ring' , similar to pC1 cells previously named 'pC1-Alpha' (Wu et al, 2019). We termed these 'pC1-Alpha-l/s' for long/short based on the length of the medial vertical projection (Fig.…”

Section: Pc1 Cell Typesmentioning

confidence: 82%

“…Thermogenetic activation of pC1 neurons in females drives chasing (Rezával et al, 2016;Wu et al, 2019), a male-specific behavior, and also aggressive behaviors toward females (Palavicino-Maggio et al, 2019). Taken together these data suggest that female pC1 neurons can drive multiple distinct behaviors, similar to male P1 neurons, but whether female pC1 neurons can drive persistent changes in behavior and persistent neural activity, has not yet been investigated.…”

Section: Introductionmentioning

confidence: 94%

“…3A-B) and excluded neurons that do not project to the lateral junction (Supp Fig. S3C-D), as all pC1 cells characterized so far project to the lateral junction (Kimura et al, 2015;Rezával et al, 2016;Wu et al, 2019;Zhou et al, 2014). We sorted pC1 cells manually based on morphology and found 7 major pC1-like cell types in the EM volume ( Fig.…”

Section: Pc1 Cell Typesmentioning

confidence: 99%

“…Little attention has been paid to the persistence of social behaviors in females. Drosophila females lack P1 neurons, but do have Doublesex+ pC1 neurons (Rideout et al, 2010;Robinett et al, 2010;Zhou et al, 2014), including a subset that are female-specific (Wu et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…The effect of pC1 activation on female receptivity peaks at a different time relative to stimulus offset, compared with effects on aggressive and male-like behaviors. We identify the subset of pC1 neurons (called pC1-Alpha (Wu et al, 2019)) that affects the persistent aggressive and male-like behaviors. Using calcium imaging, we find that pC1-Alpha activation can elicit persistent activity among multiple cell types, including pC1 neurons themselves.…”

Section: Introductionmentioning

confidence: 99%

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The Neural Basis for a Persistent Internal State inDrosophilaFemales

Deutsch

Pacheco

Encarnacion-Rivera

et al. 2020

Preprint

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Sustained changes in mood or action require persistent changes in neural activity, but it has been difficult to identify and characterize the neural circuit mechanisms that underlie persistent activity and contribute to long-lasting changes in behavior. Here, we focus on changes in the behavioral state of Drosophila females that persist for minutes following optogenetic activation of a single class of central brain neurons termed pC1. We find that female pC1 neurons drive a variety of persistent behaviors in the presence of males, including increased receptivity, shoving, and chasing. By reconstructing cells in a volume electron microscopic image of the female brain, we classify 7 different pC1 cell types and, using cell type specific driver lines, determine that one of these, pC1-Alpha, is responsible for driving persistent female shoving and chasing. Using calcium imaging, we locate sites of minutes-long persistent neural activity in the brain, which include pC1 neurons themselves. Finally, we exhaustively reconstruct all synaptic partners of a single pC1-Alpha neuron, and find recurrent connectivity that could support the persistent neural activity. Our work thus links minutes-long persistent changes in behavior with persistent neural activity and recurrent circuit architecture in the female brain.

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Section: Pc1 Cell Typesmentioning

confidence: 82%

Section: Introductionmentioning

confidence: 94%

Section: Pc1 Cell Typesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The Neural Basis for a Persistent Internal State inDrosophilaFemales

Deutsch

Pacheco

Encarnacion-Rivera

et al. 2020

Preprint

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A small number of cholinergic neurons mediate hyperaggression in female Drosophila

Palavicino‐Maggio

Chan

McKellar

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

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In the Drosophila model of aggression, males and females fight in same-sex pairings, but a wide disparity exists in the levels of aggression displayed by the 2 sexes. A screen of Drosophila Flylight Gal4 lines by driving expression of the gene coding for the temperature sensitive dTRPA1 channel, yielded a single line (GMR26E01-Gal4) displaying greatly enhanced aggression when thermoactivated. Targeted neurons were widely distributed throughout male and female nervous systems, but the enhanced aggression was seen only in females. No effects were seen on female mating behavior, general arousal, or male aggression. We quantified the enhancement by measuring fight patterns characteristic of female and male aggression and confirmed that the effect was female-specific. To reduce the numbers of neurons involved, we used an intersectional approach with our library of enhancer trap flp-recombinase lines. Several crosses reduced the populations of labeled neurons, but only 1 cross yielded a large reduction while maintaining the phenotype. Of particular interest was a small group (2 to 4 pairs) of neurons in the approximate position of the pC1 cluster important in governing male and female social behavior. Female brains have approximately 20 doublesex (dsx)-expressing neurons within pC1 clusters. Using dsxFLP instead of 357FLP for the intersectional studies, we found that the same 2 to 4 pairs of neurons likely were identified with both. These neurons were cholinergic and showed no immunostaining for other transmitter compounds. Blocking the activation of these neurons blocked the enhancement of aggression.

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The function of juvenile-adult transition axis in female sexual receptivity ofDrosophila melanogaster

Li,

Ning,

Liu

et al. 2023

Preprint

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Female sexual receptivity is essential for reproduction of a species. Neuropeptides play the main role in regulating female receptivity. However, whether neuropeptides regulate the establishment of neural circuits for female sexual receptivity is unknown. Here we found the peptide hormone prothoracicotropic hormone (PTTH), which belongs to the insect PG axis, regulated virgin female receptivity through ecdysone during neural maturation inDrosophila melanogaster. We identified PG neurons expressing PTTH as doublesex-positive neurons, they regulated virgin female receptivity before the metamorphosis during the 3rd-instar larval stage. Furthermore, the ecdysone receptor EcR-A in pC1 neurons regulated virgin female receptivity during metamorphosis. The reduced EcR-A in pC1 neurons induced abnormal morphological development of pC1 neurons without changing neural activity. Among all subtypes of pC1 neurons, the function of EcR-A in pC1b neurons was necessary for virgin female copulation rate. These suggested that the changes of synaptic connections between pC1b and other neurons decreased female copulation rate. Moreover, analysis of brain transcriptomes when EcR-A was reduced in pC1 neurons revealed that, additional genes were regulated downstream of EcR-A function in pC1 neurons. The PG axis has similar functional strategy as the HPG axis in mammals to trigger the juvenile–adult transition. Our work suggests a general mechanism underlying which the neurodevelopment during maturation regulates female sexual receptivity.

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Drosophilafemale-specific brain neuron elicits persistent position- and direction-selective male-like social behaviors

Cited by 8 publications

References 41 publications

The Neural Basis for a Persistent Internal State inDrosophilaFemales

The Neural Basis for a Persistent Internal State inDrosophilaFemales

A small number of cholinergic neurons mediate hyperaggression in female Drosophila

The function of juvenile-adult transition axis in female sexual receptivity ofDrosophila melanogaster

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