<i>Escherichia coli</i>Eyelid Abscess in a Patient with Alcoholic Cirrhosis

Stratton, Matthew S.; Capitena, Cara; Christensen, Logan; Paciuc-Beja, Miguel

doi:10.1155/2015/827609

Cited by 4 publications

(4 citation statements)

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“…With this in view, in the present study ocular isolates of E. coli which are known to cause about 1.1% of the total ocular infections [22] such as keratitis [23], endophthalmitis [24, 25], conjunctivitis [6], pan-ophthalmitis [26] and eyelid abscess [27], were used as model systems to investigate antibiotic susceptibility and biofilm formation potential. In addition global gene expression was studied by DNA-microarray analysis to identify genes that are involved in biofilm formation and antibiotic resistance.…”

Section: Introductionmentioning

confidence: 99%

Global gene expression in Escherichia coli, isolated from the diseased ocular surface of the human eye with a potential to form biofilm

et al. 2017

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Background Escherichia coli, the gastrointestinal commensal, is also known to cause ocular infections such as conjunctivitis, keratitis and endophthalmitis. These infections are normally resolved by topical application of an appropriate antibiotic. But, at times these E. coli are resistant to the antibiotic and this could be due to formation of a biofilm. In this study ocular E. coli from patients with conjunctivitis, keratitis or endophthalmitis were screened for their antibiotic susceptibility and biofilm formation potential. In addition DNA-microarray analysis was done to identify genes that are involved in biofilm formation and antibiotic resistance.ResultsOut of 12 ocular E. coli isolated from patients ten isolates were resistant to one or more of the nine antibiotics tested and majority of the isolates were positive for biofilm formation. In E. coli L-1216/2010, the best biofilm forming isolate, biofilm formation was confirmed by scanning electron microscopy. Confocal laser scanning microscopic studies indicated that the thickness of the biofilm increased up to 72 h of growth. Further, in the biofilm phase, E. coli L-1216/2010 was 100 times more resistant to the eight antibiotics tested compared to planktonic phase. DNA microarray analysis indicated that in biofilm forming E. coli L-1216/2010 genes encoding biofilm formation such as cell adhesion genes, LPS production genes, genes required for biofilm architecture and extracellular matrix remodeling and genes encoding for proteins that are integral to the cell membrane and those that influence antigen presentation are up regulated during biofilm formation. In addition genes that confer antimicrobial resistance such as genes encoding antimicrobial efflux (mdtM and cycA), virulence (insQ, yjgK), toxin production (sat, yjgK, chpS, chpB and ygjN), transport of amino-acids and other metabolites (cbrB, cbrC, hisI and mglB) are also up regulated. These genes could serve as potential targets for developing strategies for hacking biofilms and overcoming antibiotic resistance.ConclusionsThis is the first study on global gene expression in antibiotic resistant ocular E. coli with a potential to form biofilm. Using native ocular isolates for antibiotic susceptibility testing, for biofilm formation and global gene expression is relevant and more acceptable than using type strains or non clinical strains which do not necessarily mimic the native isolate.Electronic supplementary materialThe online version of this article (doi:10.1186/s13099-017-0164-2) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Global gene expression in Escherichia coli, isolated from the diseased ocular surface of the human eye with a potential to form biofilm

et al. 2017

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“…The condition can be serious to the extent of causing vision loss (Stratton et al, 2015). It can be classified as preseptal cellulitis, orbital cellulitis, subperiosteal abscess, intraorbital abscess and cavernous sinus thrombosis related cellulitis (Gonzalez and Durairaj, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Preseptal cellulitis (PC) is defined as an inflammation of the eyelid and surrounding skin with eyelid abscess being a possible outcome (Akçay et al, 2014). The most frequent pathogen implicated in the etiology of this group of ocular infections is H. influenza (Stratton et al, 2015). Currently, S. aureus and Streptococcus species cause the majority of culture positive cases of preseptal cellulitis (Gonzalez and Durairaj, 2010).…”

Section: Introductionmentioning

confidence: 99%

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External ocular bacterial infections among Sudanese children at Khartoum State, Sudan

Mohager¹,

Kaddam²,

Mohager³

2016

Afr. J. Microbiol. Res.

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Ocular infections are widespread and they exert heavy burden on eye health. Virtually, any eye component can be infected by a diversity of bacteria. The present study was performed to determine the prevalence of external ocular bacterial infections and to find out antibiotic susceptibility pattern of bacterial isolates at eye care hospitals in Khartoum, Sudan. Two hundred and four corneal scrape and drained pus materials were received from infected eyes with clinical diagnoses of bacterial conjunctivitis, keratoconjunctivitis, keratitis, blepharo-conjunctivitis, blepharitis, dacryo-cystitis and eye abscess. Culture, microscopy with Gram's stain of both samples, bacterial colonies and biochemical tests were carried out. Antibiotic susceptibility analysis using Kirby-Bauer disc diffusion test and standard table of antibiotic susceptibility was performed. Out of 204 samples processed, 130 (63.7%) yielded bacterial growth. The most prevalent bacterial eye infection was conjunctivitis (59.2%). Of all the isolates, 75 (57.7%) were Gram's positive and 55 (42.3%) were Gram's negative. Coagulase positive Staphylococcus aureus were the most prevalent, 39 (30%) followed by Streptococcus pneumonia 31 (23.8%), Haemophilus influenzae 22 (16.9%), Pseudomonas aeruginosa 13 (10%) and Neisseria gonorrheae 10 (7.7%). Gram positive bacteria were highly sensitive to vancomycin (95%), followed by chloramphenicol and ciprofloxacin (91%) and ceftriaxone (84%), while the majority were resistant to penicillin (72%). Gram-negative organisms were highly susceptible to amikacin (92.7%) followed by ceftriaxone (87.3%) and ciprofloxacin (78.2%). Major resistance was towards cotrimoxazole (82%) and ampicillin (73%).

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Risk factors for endogenous endophthalmitis in infectious endocarditis patients

Shah,

Zhu,

Uppuluri

et al. 2024

Eye

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Background/Objectives The purpose of this study was to identify demographic variables and systemic comorbidities that may increase risk of endogenous endophthalmitis (EE) development in patients with infective endocarditis (IE). Subjects/Methods A retrospective database study was conducted using the 2002–2014 National Inpatient Sample (NIS). Patients with IE and EE were identified using ICD-9-CM codes. Descriptive chi-square and logistic regression analysis identified risk factors for EE in IE patients. Results Of 769,472 inpatients with a diagnosis of IE, 2248 had a diagnosis of EE. Women comprised 39.7% of IE patients without EE and 42.6% of those with EE (p = 0.005). The majority of IE cases with EE were in those 21–64-year-old (58.5%) age cohort and 67.4% of cases were Whites. Multivariate analysis revealed IE patients in the 21–64 (OR, 3.660) and 65+ age group (OR, 2.852) had increased risk of developing EE compared to the 0–20-year-old group. Hispanic (OR, 1.377) and Asian/Pacific Islander (OR, 1.620) patients had increased risk compared to White patients. Diabetes with (OR, 2.043) and without (OR, 1.433) chronic complications, alcohol use disorder (AUD; OR, 1.795), and cirrhosis (OR, 1.452) conferred an increased risk of developing EE, whereas, congestive heart failure (CHF; OR, 0.716), arrhythmia (OR, 0.678), and having a cardiac device (OR, 0.336) decreased risk of EE in IE subjects. Conclusion Older age (21+ years) and Hispanic and Asian/Pacific Islander background were associated with increased risk of developing EE in IE patients. Diabetes with and without chronic complications, AUD, or cirrhosis also conferred a 1.5–2 times increased risk. CHF, arrhythmia, or having a cardiac device were associated with decreased risk.

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Escherichia coliEyelid Abscess in a Patient with Alcoholic Cirrhosis

Cited by 4 publications

References 10 publications

Global gene expression in Escherichia coli, isolated from the diseased ocular surface of the human eye with a potential to form biofilm

Global gene expression in Escherichia coli, isolated from the diseased ocular surface of the human eye with a potential to form biofilm

External ocular bacterial infections among Sudanese children at Khartoum State, Sudan

Risk factors for endogenous endophthalmitis in infectious endocarditis patients

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