<i>Ex vivo</i> identification of circulating tumor cells in peripheral blood by fluorometric “turn on” aptamer nanoparticles

Xia, Weiguo; Shangguan, Xiaoyan; Wang, Yan; Xi, Dongmei; Sun, Wen; Fan, Jiangli; Shao, Kun; Peng, Xiaojun

doi:10.1039/d0sc05112h

Cited by 9 publications

(5 citation statements)

References 54 publications

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“…[162] Yet, the precise discernment of exceedingly scarce CTCs amidst intricate peripheral blood milieu remains a formidable challenge. [163] In a pioneering endeavor, Feng et al introduce an innovative electrochemical cell sensor underpinned by AuIrPt nanozymes, orchestrated via a tripartite recognition strategy tailored for the precise profiling of CTCs. [164] This trinity of AuIrPt nanozymes endows the sensor with the mimicked functions of SOD, POD and CAT.…”

Section: Detecting Of Cellsmentioning

confidence: 99%

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Nanozyme‐Enhanced Electrochemical Biosensors: Mechanisms and Applications

Yang,

Guo,

Wang

et al. 2023

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Nanozymes, as innovative materials, have demonstrated remarkable potential in the field of electrochemical biosensors. This article provides an overview of the mechanisms and extensive practical applications of nanozymes in electrochemical biosensors. First, the definition and characteristics of nanozymes are introduced, emphasizing their significant role in constructing efficient sensors. Subsequently, several common categories of nanozyme materials are delved into, including metal‐based, carbon‐based, metal‐organic framework, and layered double hydroxide nanostructures, discussing their applications in electrochemical biosensors. Regarding their mechanisms, two key roles of nanozymes are particularly focused in electrochemical biosensors: selective enhancement and signal amplification, which crucially support the enhancement of sensor performance. In terms of practical applications, the widespread use of nanozyme‐based electrochemical biosensors are showcased in various domains. From detecting biomolecules, pollutants, nucleic acids, proteins, to cells, providing robust means for high‐sensitivity detection. Furthermore, insights into the future development of nanozyme‐based electrochemical biosensors is provided, encompassing improvements and optimizations of nanozyme materials, innovative sensor design and integration, and the expansion of application fields through interdisciplinary collaboration. In conclusion, this article systematically presents the mechanisms and applications of nanozymes in electrochemical biosensors, offering valuable references and prospects for research and development in this field.

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Section: Detecting Of Cellsmentioning

confidence: 99%

“…[ 162 ] Yet, the precise discernment of exceedingly scarce CTCs amidst intricate peripheral blood milieu remains a formidable challenge. [ 163 ]…”

Section: Practical Applications Of Nanozymes Electrochemical Sensorsmentioning

confidence: 99%

Nanozyme‐Enhanced Electrochemical Biosensors: Mechanisms and Applications

Yang,

Guo,

Wang

et al. 2023

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“…These biorecognition elements exhibit a high affinity to antigens on the cancer cells' surface, thereby making specific bonding with cancer cells. 23,24 Due to their outstanding selectivity, antibodies present the most popular biorecognition elements of cytosensors. Despite the popularity of using antibodies, their applications are limited due to: (i) their complex utilisation procedure, (ii) unstable recognition capability in harsh chemical environments, such as highly alkaline conditions, (iii) nondirectional coupling of antibodies to the solid support that reduces their efficiency in cell capturing 25 and (iv) their high cost.…”

Section: Biorecognition Elements In the Detection Of Ctcsmentioning

confidence: 99%

Optical cytosensors for the detection of circulating tumour cells

et al. 2022

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“…[8] For instance, the use of anti-EpCAM antibody/aptamer as a capture probe is one of the most studied methods for selectively capturing CTCs with high expression EpCAM (e.g., human breast cancer MCF-7 cells), however, it cannot capture CTCs with low expression EpCAM due to EMT or CTCs without EpCAM expression (e.g., human cervical cancer HeLa cells), resulting in inaccurate detection. [4] In addition, the concentration of CTCs in peripheral blood is extremely low, and it may be even lower in cancer patients without significant metastasis, [23][24][25] which further restricts the clinical application of LMMs. For efficient and ultrasensitive detection of heterogeneous CTCs, it is crucial to develop a multifunction LMM system that integrates high specificity, ideal antileukocyte adsorption performance, and signal amplification strategy.…”

Section: Introductionmentioning

confidence: 99%

Engineering Biomimetic Biosensor Using Dual‐Targeting Multivalent Aptamer Regulated 3D DNA Walker Enables High‐Performance Detection of Heterogeneous Circulating Tumor Cells

Jia,

Hu,

Zhang

et al. 2023

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The phenotypic heterogeneity of circulating tumor cells (CTCs) and the nonspecific adsorption of background cells impede the effective and sensitive detection of rare CTCs. Although leukocyte membrane coating approach has a good antileukocyte adhesion ability and holds great promise for addressing the challenge of capture purity, its limited specificity and sensitivity prevent its use in the detection of heterogeneous CTCs. To overcome these obstacles, a biomimetic biosensor that integrated dual‐targeting multivalent aptamer/walker duplex functionalized biomimetic magnetic beads and an enzyme‐powered DNA walker signal amplification strategy is designed. As compared to conventional leukocyte membrane coating, the biomimetic biosensor achieves efficient and high purity enrichment of heterogeneous CTCs with different epithelial cell adhesion molecule (EpCAM) expression while minimizing the interference of leukocytes. Meanwhile, the capture of target cells can trigger the release of walker strands to activate an enzyme‐powered DNA walker, resulting in cascade signal amplification and the ultrasensitive and accurate detection of rare heterogeneous CTCs. Importantly, the captured CTCs remained viable and can be recultured in vitro with success. Overall, this work provides a new perspective for the efficient detection of heterogeneous CTCs by biomimetic membrane coating and paves the way for early cancer diagnosis.

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Ex vivo identification of circulating tumor cells in peripheral blood by fluorometric “turn on” aptamer nanoparticles

Abstract: The detection of the circulating tumor cells (CTCs) detached from solid tumors has emerged as a burgeoning topic for cancer diagnosis and treatment.

Cited by 9 publications

References 54 publications

Nanozyme‐Enhanced Electrochemical Biosensors: Mechanisms and Applications

Nanozyme‐Enhanced Electrochemical Biosensors: Mechanisms and Applications

Optical cytosensors for the detection of circulating tumour cells

Engineering Biomimetic Biosensor Using Dual‐Targeting Multivalent Aptamer Regulated 3D DNA Walker Enables High‐Performance Detection of Heterogeneous Circulating Tumor Cells

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