<i>Ex vivo</i>stimulation of cord blood mononuclear cells by dexamethasone and interleukin-7 results in the maturation of interferon-γ-secreting effector memory T cells

Talayev, V. Yu.; Zaichenko, I.Ye.; Babaykina, O.N.; Lomunova, Maria; Talayeva, E B; Никонова, М Ф

doi:10.1111/j.1365-2249.2005.02863.x

Cited by 6 publications

(3 citation statements)

References 39 publications

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“…However, 72 h of incubation resulted in elevated background IFN-␥ production such that naïve T cells could have been primed (29). IL-7 and dexamethasone treatment of cord blood cells causes maturation of T cells into effector memory cells (46). IL-7 also promotes transition of T effectors to T memory cells, at least in the mouse (27).…”

Section: Vol 15 2008 Il-7 Enhances Memory T-cell Responses 1617mentioning

confidence: 99%

Enhancement of Human Antigen-Specific Memory T-Cell Responses by Interleukin-7 May Improve Accuracy in Diagnosing Tuberculosis

Feske

Nudelman

Medina

et al. 2008

Clin Vaccine Immunol

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Section: Vol 15 2008 Il-7 Enhances Memory T-cell Responses 1617mentioning

confidence: 99%

Enhancement of Human Antigen-Specific Memory T-Cell Responses by Interleukin-7 May Improve Accuracy in Diagnosing Tuberculosis

Feske

Nudelman

Medina

et al. 2008

Clin Vaccine Immunol

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“…Then, the cells were stained with fluorescent conjugates of antibodies to the molecules of CD4 (Sorbent, Russia), CD45RO, CD196 (CCR6) (BioLegend), and CD197 (CCR7) (eBioscience, USA) and fixated with 4% paraformaldehyde. The membranes were permeabilized with 0.1% saponin followed by cell staining for cytokines using conjugates of antibodies to IFN-g and IL-17A (eBioscience), as described previously [ 21 ]. The samples were analyzed using a flow cytometer FacsCanto II (BD Biosciences, USA), aiming at evaluating the number of IFN-g and IL-17A producers in CD4 + , CD4 + CD45RO + , and CD4 + CD45RO + CSR6 + CCR7 – cells.…”

Section: Methodsmentioning

confidence: 99%

Cytokine Profile of CCR6+ T-Helpers Isolated from the Blood of Patients with Peptic Ulcer Associated with Helicobacter pylori Infection

Talayev¹,

Svetlova²,

Zaichenko³

et al. 2020

Sovrem Tehnol Med

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We previously found that the number of CCR6 + T-helpers with the phenotype of effector/effector memory T cells increases in the blood of patients with H. pylori -associated peptic ulcer. The mature phenotype and the expression of the chemokine receptor CCR6, which is involved in migration of lymphocytes to the inflamed mucous membrane of the gastrointestinal tract, suggests that these cells are involved in the immune response observed in this clinical condition. To better understand the pathogenetic role of these cells, it is necessary to study their functional activity, specifically, the production of pro-inflammatory cytokines involved in the pathogenesis of the disease. The aim of the study was to evaluate changes in the blood level of pro-inflammatory types of mature CCR6 + T-helpers in H. pylori -associated peptic ulcer disease. Materials and Methods. CCR6 + T-helpers were isolated from the blood by using immuno-magnetic separation adapted to this study. The number of T-helpers of types 1 and 17 (Th1 and Th17) and cells with mixed properties of Th1 and Th17 (Th1/Th17) was determined by intracellular cytokine assay. Results. Initially, we planned to activate unseparated peripheral blood mononuclear cells ex vivo and evaluate the number of cytokine producers among mature CCR6 + T-helper cells by gating them during the flow cytometry. However, dramatic changes in the phenotype of T-helpers upon activation did not allow us to reliably identify the cells of interest . Subsequently, we used a two-stage immunomagnetic separation procedure to obtain functionally active mature CCR6 + T-helpers with a purity of >90%. The quantitative yield of these cells from the blood of patients with gastric and duodenal peptic ulcer associated with H. pylori was 9 times higher than that from the blood of healthy donors. Activation of CCR6 + T-helpers purified from blood of ulcer patients revealed an increased content of Th1, Th17, and Th1/Th17. One ml of the patient’s blood yielded 18.1 times more CCR6 + Th1, 19.4 times more CCR6 + Th17, and 21.1 times more CCR6 + Th1/Th17 compared with the blood of healthy subjects. Conclusion. The content of mature CCR6 + T-helper cells with pro-inflammatory activity significantly increases in the blood of patients with peptic ulcer associated with H. pylori infection.

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“…In addition to playing a critical role in peripheral immune homeostasis [9] , [14] , [15] , [16] , [17] , [18] and the development and maintenance of T-cell memory, [19] , [20] IL-7 also plays an important role in the activation of CD8 T-cells in response to foreign antigen. IL-7 independently stimulates CD8 T-cell proliferation, [21] , [22] , [23] , [24] and potentiates cytolytic activity [25] , [26] , [27] , [28] , [29] , [30] , [31] by enhancing production of IFN-γ following TCR stimulation [32] , [33] and by inducing accumulation of intracellular perforin. [34] , [35] Given the important role IL-7 plays in CD8 T-cell responses, decreased IL-7 signaling would be expected to result in impaired cell mediated immunity and inefficient control of viral pathogens including HIV.…”

Section: Introductionmentioning

confidence: 99%

IL-7 Receptor Recovery on CD8 T-Cells Isolated from HIV+ Patients Is Inhibited by the HIV Tat Protein

2014

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Expression of the IL-7 receptor α-chain (CD127) is decreased on CD8 T-cells in HIV infected patients and partially recovers in those receiving antiretroviral therapy with sustained viral suppression. We have shown that soluble HIV Tat protein down regulates CD127 expression on CD8 T-cells isolated from healthy HIV-negative individuals. Tat is taken up by CD8 T-cells via endocytosis, exits the endosome and then translocates to the inner leaflet of the cell membrane where it binds to the cytoplasmic tail of CD127 inducing receptor internalization and degradation by the proteasome. This down regulation of CD127 by Tat results in impaired CD8 T-cell function. Interestingly, suppression of CD127 by Tat is reversible and requires the continual presence of Tat in the culture media. We thus questioned whether the low IL-7 receptor expression evident on CD8 T-cells in HIV+ patients was similarly reversible and if suppression of the receptor could be maintained ex vivo by Tat protein alone. We show here that when CD8 T-cells isolated from HIV+ patients are incubated alone in fresh medium, low CD127 expression on the cell surface recovers to normal levels. This recovery of CD127, however, is completely inhibited by the addition of HIV Tat protein to the culture media. This study then provides evidence that soluble factor(s) are responsible for low CD127 expression on circulating CD8 T-cells in HIV+ individuals and further implicates Tat in suppressing this receptor essential to CD8 T-cell proliferation and function.

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Ex vivostimulation of cord blood mononuclear cells by dexamethasone and interleukin-7 results in the maturation of interferon-γ-secreting effector memory T cells

Cited by 6 publications

References 39 publications

Enhancement of Human Antigen-Specific Memory T-Cell Responses by Interleukin-7 May Improve Accuracy in Diagnosing Tuberculosis

Enhancement of Human Antigen-Specific Memory T-Cell Responses by Interleukin-7 May Improve Accuracy in Diagnosing Tuberculosis

Cytokine Profile of CCR6+ T-Helpers Isolated from the Blood of Patients with Peptic Ulcer Associated with Helicobacter pylori Infection

IL-7 Receptor Recovery on CD8 T-Cells Isolated from HIV+ Patients Is Inhibited by the HIV Tat Protein

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