Fasciola hepaticain Some Buffaloes and Cattle by PCR and Microscopy

Abstract

Fasciolosis is the burning problem of the livestock rearing community having huge morbidity, mortality, and economic losses to livestock industries in our country Pakistan. The faecal and liver biopsy samplings were examined by polymerase chain reaction (PCR) and microscopy technique during the entire study. A total of 307 samples including 149 samples from Karak and 158 samples from Kohat abattoirs were examined by PCR method and overall prevalence of fasciolosis was 5.86% (18/307), amongst theses 8.05% (12/149) in liver biopsy and 3.79% (6/158) in feacal samples of cattle and Buffaloes were recorded. Similarly the microscopy based detection was 3.58% (11/307) including 4.61% (7/149) in liver biopsy and 2.5% (4/158) in faecal samples accordingly. Furthermore the areawise prevalence of fasciolosis in abattoirs by PCR method was found to be 7.59% (12/158) in Kohat and 4.02% (6/149) in Karak. A 618 pb DNA was amplified in 2% agarose gel electrophoreses. It is concluded from the study that prevalence of fasciolosis was higher in abattoir of district Kohat and PCR was a more sensitive method of diagnosis than microscopy.

Biochemical and immunological investigation of fascioliasis in cattle in Egypt

“…Fascioliasis is an extremely serious disease that causes enormous losses in cattle production and in the related meat, milk, and leather industry as Fascciola spp. could be found in ectopic location as the skin [15]. Although the prevalence of fascioliasis recorded in the present study was low (16.81%), which may not be a regular consideration of its prevalence in New Valley, Egypt, analysis of the different immunological and biochemical parameters emphasizes on how the infestation of F. hepatica even at such a low prevalence rate affects the quality of meat and meat products, particularly when apparently clinically normal animals are allowed to be slaughtered after antemortem examination.…”

“…In the present study, only F. hepatica was detected at the study site (100%), as revealed by postmortem examination, whereas F. gigantica and mixed specifies were not detected, which is consistent with that reported by Tulu [24], who stated that F. hepatica (100%) was the predominant species of zoonotic liver flukes found at an abattoir in Ambo district, Ethiopia. The prevalence rate of F. hepatica can reach up to 77% in developing countries [15].…”

Fasciola and fasciolosis in ruminants in Europe: Identifying research needs

“…Robles‐Pérez, Martínez‐Pérez, Rojo‐Vázquez, and Martínez‐Valladares () also detected the infection at 2 wpi but by a conventional PCR, which is a less time consuming method, and amplifying a 292 bp fragment of the ITS2 gene. Ayaz, Ullah, AbdEl‐Salam, Shams, and Niaz () compared the prevalence of F. hepatica in cattle and buffaloes using the FEC and a PCR method; authors showed the higher sensitivity of the molecular technique. Additionally, to differentiate between species, F. hepatica and F. gigantica , a single‐step multiplex PCR was developed for simultaneous detection using faecal samples (Le et al., ); both species overlap in distribution in some countries of Africa and Asia and have similar egg morphology, making identification from faecal samples difficult.…”

Accurate and rapid detection of Fasciola hepatica copro‐DNA in sheep using loop‐mediated isothermal amplification (LAMP) technique

“…Ayaz et al. (2014) compared the PCR and microscopy for the diagnosis of F. hepatica in some buffaloes and cattle. Their results showed that PCR was a more sensitive method of Fasciola diagnosis than microscopy.…”