<i>FOXE1</i>
 is a target for aberrant methylation in cutaneous squamous cell carcinoma

Venza, Isabella; Visalli, Maria; Tripodo, B.; Grazia, Giuseppina De; Loddo, Saverio; Teti, Diana

doi:10.1111/j.1365-2133.2009.09560.x

Cited by 52 publications

(30 citation statements)

References 31 publications

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“…In thyroid cancer, FOXE1 is upregulated (35), although most cancers have a decreased expression of FOXE1 often due to promoter CpG island methylation as has been described in breast cancer (36), pancreatic cancer (37), and in cutaneous squamous cell carcinoma (38). Furthermore, FOXE1 has been reported as a sensitive (82%) and specific (98.5%) methylation marker for pancreatic cancer in pancreatic juice of patients (37).…”

Section: Discussionmentioning

confidence: 99%

Spectrin Repeat Containing Nuclear Envelope 1 and Forkhead Box Protein E1 Are Promising Markers for the Detection of Colorectal Cancer in Blood

Melotte

Lentjes

et al. 2015

Cancer Prevention Research

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Identifying biomarkers in body fluids may improve the noninvasive detection of colorectal cancer. Previously, we identified N-Myc downstream-regulated gene 4 (NDRG4) and GATA binding protein 5 (GATA5) methylation as promising biomarkers for colorectal cancer in stool DNA. Here, we examined the utility of NDRG4, GATA5, and two additional markers [Forkhead box protein E1 (FOXE1) and spectrin repeat containing nuclear envelope 1 (SYNE1)] promoter methylation as biomarkers in plasma DNA. Quantitative methylation-specific PCR was performed on plasma DNA from 220 patients with colorectal cancer and 684 noncancer controls, divided in a training set and a test set. Receiver operating characteristic analysis was performed to measure the area under the curve of GATA5, NDRG4, SYNE1, and FOXE1 methylation. Functional assays were performed in SYNE1 and FOXE1 stably transfected cell lines. The sensitivity of NDRG4, GATA5, FOXE1, and SYNE1 methylation in all stages of colorectal cancer (154 cases, 444 controls) was 27% [95% confidence interval (CI), 20%–34%), 18% (95% CI, 12%–24%), 46% (95% CI, 38%– 54%), and 47% (95% CI, 39%–55%), with a specificity of 95% (95% CI, 93%–97%), 99% (95% CI, 98%–100%), 93% (95% CI, 91%–95%), and 96% (95% CI, 94%–98%), respectively. Combining SYNE1 and FOXE1, increased the sensitivity to 56% (95% CI, 48%–64%), while the specificity decreased to 90% (95% CI, 87%–93%) in the training set and to 58% sensitivity (95% CI, 46%–70%) and 91% specificity (95% CI, 80%–100%) in a test set (66 cases, 240 controls). SYNE1 overexpression showed no major differences in cell proliferation, migration, and invasion compared with controls. Overexpression of FOXE1 significantly decreased the number of colonies in SW480 and HCT116 cell lines. Overall, our data suggest that SYNE1 and FOXE1 are promising markers for colorectal cancer detection.

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Section: Discussionmentioning

confidence: 99%

Spectrin Repeat Containing Nuclear Envelope 1 and Forkhead Box Protein E1 Are Promising Markers for the Detection of Colorectal Cancer in Blood

Melotte

Lentjes

et al. 2015

Cancer Prevention Research

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“…Consistently, FOXO3 acts as a transcriptional eff ector of VEGF signaling in endothelial cells through a PI3K/AKT-dependent pathway [ 36 , 37 ] . FOXE1 is a thyroid-specifi c factor presumably involved in thyroid and cutaneous squamous cell carcinoma [ 38 ] , but not described in EOC to date. FOXE1 is able to modify chromatin accessibility [ 39 ] .…”

mentioning

confidence: 99%

Nerve Growth Factor Stimulates Cellular Proliferation of Human Epithelial Ovarian Cancer

Urzúa¹,

Tapia

Geraldo

et al. 2012

Horm Metab Res

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Due to its ability to induce vascular endothelial growth factor expression and proliferation, migration, and vasculogenesis of endothelial cells, nerve growth factor (NGF) has been considered as an angiogenic factor in epithelial ovarian cancer (EOC). In this work, we evaluated the angiogenic and proliferative mRNA expression profiles of EOC and addressed the responsiveness of EOC explants to NGF stimulation. Twenty EOC samples were obtained from Obstetrics and Gynecology Department, University of Chile's Clinical Hospital. Global gene expression profiles of selected poorly differentiated serous EOC samples were obtained with DNA oligonucleotide microarrays. In addition, EOC explants were subjected to NGF stimulation and levels of p-AKT, BAX, BCL2, Ki-67, c-MYC, and FOXL2 proteins were determined by immunohistochemistry. Results showed that mRNAs coding for specific transcriptional regulators and antiapoptotic components of the NGF signaling pathway were upregulated in EOC cells. At the protein level, key members of the NGF pathway including p-AKT, BCL2/BAX, Ki-67, and c-MYC were found increased, while FOXL2 was decreased in response to NGF stimulation. These findings strongly suggest that NGF stimulates cellular proliferation of human EOC.

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“…Tumor progression involved chromatin-mediated changes such as DNA methylation yet the role of histone variants in tumorogenesis is unclear. All of these modifications of gene expression have been associated with the development of various tumor types, including HNSCC and cSCC [39, 40]. A higher frequency of FOXE1 promoter hypermethylation was found in cSCC compared to normal skin, indicating that FOXE1 may be a target for aberrant methylation in cSCC [39].…”

Section: Pathogenesismentioning

confidence: 99%

Molecular Markers in Cutaneous Squamous Cell Carcinoma

Tufaro

Chuang

Prasad

et al. 2011

International Journal of Surgical Oncology

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Nonmelanoma skin carcinoma (NMSC) is the most frequent cancer in the USA with over 1.3 million new diagnoses a year; however due to an underappreciation of its associated mortality and growing incidence and its ability to be highly aggressive, the molecular mechanism is not well delineated. Whereas the molecular profiles of melanoma have been well characterized, those for cutaneous squamous cell carcinoma (cSCC) have trailed behind. This importance of the new staging paradigm is linked to the ability currently to better clinically cluster similar biologic behavior in order to risk-stratify lesions and patients. In this paper we discuss the trends in NMSC and the etiologies for the subset of NMSC with the most mortality, cutaneous SCC, as well as where the field stands in the discovery of a molecular profile. The molecular markers are highlighted to demonstrate the recent advances in cSCC.

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FOXE1 is a target for aberrant methylation in cutaneous squamous cell carcinoma

Abstract: These results indicate that FOXE1 is a crucial player in development of cutaneous SCC.

Cited by 52 publications

References 31 publications

Spectrin Repeat Containing Nuclear Envelope 1 and Forkhead Box Protein E1 Are Promising Markers for the Detection of Colorectal Cancer in Blood

Spectrin Repeat Containing Nuclear Envelope 1 and Forkhead Box Protein E1 Are Promising Markers for the Detection of Colorectal Cancer in Blood

Nerve Growth Factor Stimulates Cellular Proliferation of Human Epithelial Ovarian Cancer

Molecular Markers in Cutaneous Squamous Cell Carcinoma

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