Gaeumannomyces graminisvars.avenae, graminis, andtriticiIdentified Using PCR Amplification of Avenacinase-like Genes

Rachdawong, Sansanalak; Cramer, Carole L.; Grabau, Elizabeth A.; Stromberg, Verlyn K.; Lacy, George H.; Stromberg, Erik L.

doi:10.1094/pdis.2002.86.6.652

Cited by 23 publications

(15 citation statements)

References 33 publications

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“…For analysis of R. secalisinfected samples, the primers RS8mod2 (CCAGAG-GACCACAACTCTTGTT) and RS9mod2 (AAGG CGCAATGTGCGTTCAAA) were used to amplify a fragment from the 5.8S RNA gene of R. secalis (Lee et al 2001). For analysis of G. graminis-infected samples, the primers AV3 (TGCTCATGGTGG TTCCTGCG) and Ggt_for1 (GGTCAGGACAAG GACTACCC) were used to amplify a fragment from the Avenacinase-like gene of G. graminis (Rachdawong et al 2002). Each 96-well PCR plate contained the standard curve samples, non-template control and samples to be analyzed in threefold replicates.…”

Section: Quantitative Real-time Pcrmentioning

confidence: 99%

Response of barley to root colonization byPseudomonassp. DSMZ 13134 under laboratory, greenhouse, and field conditions

Fröhlich

Buddrus-Schiemann

Durner

et al. 2012

Journal of Plant Interactions

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Beneficial rhizobacteria strains are of substantial interest as biological plant protection agents in agriculture. Bacteria of the genus Pseudomonas have been studied for many years for their role in plant growth and biocontrol. In this study, we analyzed the influence of the commercially available agent Proradix † , which contains the strain Pseudomonas sp. DSMZ 13134, on barley. In controlled infection experiments, we showed that Pseudomonas sp. DSMZ 13134 induces resistance to the barley leaf pathogen Rhynchosporium secalis and inhibits the growth of the barley root pathogen Gaeumannomyces graminis. In greenhouse experiments, Pseudomonas sp. DSMZ 13134-treated plants showed enhanced growth and yield under nutrient deprivation. In field trials, an increase of yield and straw weight was observed. While the quality of grains, as determined by starch and protein content, was not affected, the yield increased by up to 20%. Our results demonstrate the value of Pseudomonas sp. DSMZ 13134 in agronomical applications for barley.

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Section: Quantitative Real-time Pcrmentioning

confidence: 99%

Response of barley to root colonization byPseudomonassp. DSMZ 13134 under laboratory, greenhouse, and field conditions

Fröhlich

Buddrus-Schiemann

Durner

et al. 2012

Journal of Plant Interactions

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“…Specific PCR assays for identification of Ggt, Ggg and Gga, based on avenacinase and avenacinase‐like gene sequences, have recently been developed (Rachdawong et al ., 2002). Variety‐specific forward PCR primers were used, singly and together, with a common reverse PCR primer to give amplification products of different sizes from DNA of Ggt, Ggg and Gga isolates.…”

Section: Detection and Identification Of G Graminis Varieties By Dnamentioning

confidence: 99%

“…The avenacinase‐minus mutant was no longer pathogenic on oats but was still able to infect wheat. Ggt and some other members of the Gaeumannomyces–Phialophora complex also contain DNA sequences homologous to the avenacinase gene even though they are unable to inactivate avenacin effectively (Osbourn, 1996a; Osbourn et al ., 1994a; Rachdawong et al ., 2002). The role of these avenacinase‐like genes is uncertain but it has been suggested that they might be involved in producing enzymes that detoxify other saponins in hosts other than oats (e.g.…”

Section: Factors Implicated In Pathogenicity and Host Range Determinamentioning

confidence: 99%

Gaeumannomyces graminis, the take‐all fungus and its relatives

Freeman

Ward

2004

Molecular Plant Pathology

106

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SUMMARY Take‐all, caused by the fungus Gaeumannomyces graminis var. tritici, is the most important root disease of wheat worldwide. Many years of intensive research, reflected by the large volume of literature on take‐all, has led to a considerable degree of understanding of many aspects of the disease. However, effective and economic control of the disease remains difficult. The application of molecular techniques to study G. graminis and related fungi has resulted in some significant advances, particularly in the development of improved methods for identification and in elucidating the role of the enzyme avenacinase as a pathogenicity determinant in the closely related oat take‐all fungus (G. graminis var. avenae). Some progress in identifying other factors that may be involved in determining host range and pathogenicity has been made, despite the difficulties of performing genetic analyses and the lack of a reliable transformation system.

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“…Recently, G. graminis var. avenae (E.M. Turner) Dennis was described as affecting oat in Chile (Gutiérrez et al, 2007); however, this pathogen also may affect wheat (Rachdawong et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Distribution and prevalence of crown rot pathogens affecting wheat crops in southern Chile

Moya-Elizondo¹,

Arismendi²,

Castro

et al. 2015

Chilean J. Agric. Res.

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Crown rot pathogens are associated with higher losses for wheat crop farmers, but information about the distribution and prevalence of these pathogens in Chile is inadequate. Distribution and prevalence of wheat (Triticum aestivum L.) crown rot pathogens were examined in a survey of 48 commercial fields from December 2011 to February 2012 in southern Chile. These fields were located between Collipulli (37º56'00" S; 72°26'39" W) and Purranque (40º50'30" S; 73°22'03" W). Severity of crown rot disease was determined through visual assessment of the first internode of 20 tillers obtained from each field. Incidence of crown rot pathogens per field was determined by plating the 20 tillers on Petri plates with 20% potato dextrose agar amended with lactic acid (aPDA) medium. Resulting fungal colonies from monoxenic culture were identified by morphological or molecular-assisted identification. Severity of crown rot varied between 11.3% and 80% for individual fields. Culture plate analysis showed 72.2% of stems were infected with some fungus. Fusarium avenaceum, F. graminearum, and F. culmorum, pathogens associated with Fusarium crown rot disease were isolated from 13.5% of tillers. Gaeumannomyces graminis, causal agent of take-all disease in cereals, was isolated from 11.1% of culms. Phaeosphaeria sp., an endophyte and possibly a non-pathogenic fungus, was isolated from 13.9% of tillers. Pathogenic fungi such as Rhizoctonia spp. and Microdochium nivale, other saprophyte, and several unidentified non-sporulating fungi were isolated at frequencies lower than 3% of the total. Fusarium crown rot and take-all were the most prevalent and distributed crown rot diseases present in wheat crops in southern Chile.

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Gaeumannomyces graminisvars.avenae, graminis, andtriticiIdentified Using PCR Amplification of Avenacinase-like Genes

Cited by 23 publications

References 33 publications

Response of barley to root colonization byPseudomonassp. DSMZ 13134 under laboratory, greenhouse, and field conditions

Response of barley to root colonization byPseudomonassp. DSMZ 13134 under laboratory, greenhouse, and field conditions

Gaeumannomyces graminis, the take‐all fungus and its relatives

Distribution and prevalence of crown rot pathogens affecting wheat crops in southern Chile

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