<i>GCR1</i>
            , the putative
            <i>Arabidopsis</i>
            G protein-coupled receptor gene is cell cycle-regulated, and its overexpression abolishes seed dormancy and shortens time to flowering

Colucci, Gabriella; Apone, Fabio; Alyeshmerni, Nicole; Chalmers, Derek T.; Chrispeels, Maarten J.

doi:10.1073/pnas.072087699

Cited by 97 publications

(74 citation statements)

References 44 publications

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“…No additional vegetative phenotypes were observed in gcr1 gpa1, agb1 gcr1, and agb1 gcr1 gpa1 mutants. Colucci et al (2002) showed that plants ectopically expressing GCR1 flower early providing a clear prediction that plants lacking a functional GCR1 would flower late. However, gcr1 null mutants typically do not flower later than wild-type plants, and, under certain conditions, actually flower slightly, but statistically earlier, than wild-type plants (data not shown).…”

Section: And B)mentioning

confidence: 99%

“…Although a ligand for GCR1 has not been identified, there is biochemical evidence that GCR1 does physically interact with GPA1 (Pandey and Assmann, 2004). Colucci et al (2002) reported that one of the phenotypes caused by overexpression of GCR1 in Arabidopsis is loss of seed dormancy. Expression of a germination marker, a phosphatase PP2A subunit, correlated with GCR1 overexpression (Colucci et al, 2002).…”

mentioning

confidence: 99%

“…Colucci et al (2002) reported that one of the phenotypes caused by overexpression of GCR1 in Arabidopsis is loss of seed dormancy. Expression of a germination marker, a phosphatase PP2A subunit, correlated with GCR1 overexpression (Colucci et al, 2002). In a follow-up study using BY2 cells overexpressing GCR1, Apone et al (2003) concluded that GCR1 regulates DNA synthesis through activation of phosphatidylinositol-specific phospholipase C.…”

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confidence: 99%

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GCR1 Can Act Independently of Heterotrimeric G-Protein in Response to Brassinosteroids and Gibberellins in Arabidopsis Seed Germination

et al. 2004

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Signal recognition by seven-transmembrane (7TM) cell-surface receptors is typically coupled by heterotrimeric G-proteins to downstream effectors in metazoan, fungal, and amoeboid cells. Some responses perceived by 7TM receptors in amoeboid cells and possibly in human cells can initiate downstream action independently of heterotrimeric G-proteins. Plants use heterotrimeric G-protein signaling in the regulation of growth and development, particularly in hormonal control of seed germination, but it is not yet clear which of these responses utilize a 7TM receptor. Arabidopsis GCR1 has a predicted 7TM-spanning domain and other features characteristic of 7TM receptors. Loss-of-function gcr1 mutants indicate that GCR1 plays a positive role in gibberellin-(GA) and brassinosteroid-(BR) regulated seed germination. The null mutants of GCR1 are less sensitive to GA and BR in seed germination. This phenotype is similar to that previously observed for transcript null mutants in the Ga-subunit, gpa1. However, the reduced sensitivities toward GA and BR in the single gcr1, gpa1, and agb1 (heterotrimeric G-protein b-subunit) mutants are additive or synergistic in the double and triple mutants. Thus, GCR1, unlike a typical 7TM receptor, apparently acts independently of the heterotrimeric G-protein in at least some aspects of seed germination, suggesting that this alternative mode of 7TM receptor action also functions in the plant kingdom.Signaling through heterotrimeric G-proteins is highly conserved among divergent eukaryotes. G-proteins physically couple the recognition of many extracellular signals by cell-surface receptors to activation of enzyme activities in the cytoplasm. In the classical paradigm, ligand binding to its cognate GPCR activates receptor-mediated GDP/GTP exchange on the a-subunit (Ga), causing dissociation of Ga from the bg dimer (Gbg). Activated Ga-subunits, Gbg, or both then bind to downstream target proteins, which results in the relevant cellular responses (Gilman, 1987). There are 23 Ga-, 6 Gb-, and 12 Gg-subunits in humans (Vanderbeld and Kelly, 2000). In contrast to humans, the Arabidopsis genome contains genes encoding only one prototypical G-protein a-subunit (GPA1), one G-protein b-subunit (AGB1), and two G-protein g-subunits (AGG1 and AGG2), indicating that the repertoire of heterotrimeric G-protein complexes in plants is smaller (Assmann, 2002;Jones, 2002). Studies on the null alleles of GPA1 and AGB1 suggest that plants use heterotrimeric G-protein signaling in many growth and developmental processes (Ullah et al., , 2002(Ullah et al., , 2003Wang et al., 2001;Chen et al., 2003).No classical GPCR has been definitively identified in plants. To date, the most promising candidate for a plant GPCR remains GCR1, independently cloned by two groups (Josefsson and Rask, 1997; PlakidouDymock et al., 1998). GCR1 encodes a protein with predicted seven membrane-spanning domains and has some sequence similarity to Dictyostelium cAMP receptors. GCR1 was originally proposed to be a receptor for cytokinins (Plakid...

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Section: And B)mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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GCR1 Can Act Independently of Heterotrimeric G-Protein in Response to Brassinosteroids and Gibberellins in Arabidopsis Seed Germination

et al. 2004

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“…These cAMP receptors are not clustered with the Rhodopsin-like family, but GCR1 possesses a motif similar to the D-R-Y triplet (H-R-T). On the basis of transgenic studies Colucci et al (2002) recently suggested that GCR1 may be the gibberellic acid receptor.…”

Section: Putative Cor413 Functionmentioning

confidence: 99%

Expression Profiling and Bioinformatic Analyses of a Novel Stress-Regulated Multispanning Transmembrane Protein Family from Cereals and Arabidopsis,

et al. 2003

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Cold acclimation is a multigenic trait that allows hardy plants to develop efficient tolerance mechanisms needed for winter survival. To determine the genetic nature of these mechanisms, several cold-responsive genes of unknown function were identified from cold-acclimated wheat (Triticum aestivum). To identify the putative functions and structural features of these new genes, integrated genomic approaches of data mining, expression profiling, and bioinformatic predictions were used. The analyses revealed that one of these genes is a member of a small family that encodes two distinct groups of multispanning transmembrane proteins. The cold-regulated (COR)413-plasma membrane and COR413-thylakoid membrane groups are potentially targeted to the plasma membrane and thylakoid membrane, respectively. Further sequence analysis of the two groups from different plant species revealed the presence of a highly conserved phosphorylation site and a glycosylphosphatidylinositol-anchoring site at the C-terminal end. No homologous sequences were found in other organisms suggesting that this family is specific to the plant kingdom. Intraspecies and interspecies comparative gene expression profiling shows that the expression of this gene family is correlated with the development of freezing tolerance in cereals and Arabidopsis. In addition, several members of the family are regulated by water stress, light, and abscisic acid. Structure predictions and comparative genome analyses allow us to propose that the cor413 genes encode putative G-protein-coupled receptors.

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“…In contrast to animals, where they may number in the hundreds, plant G-protein subunits are coded by only one or sometimes two genes (Ma et al, 1990;Marsh and Kaufman, 1999;Assmann, 2002). Analysis of Arabidopsis plants mutant in GPA1 gene reveal that G proteins are likely to be involved in a number of processes critical for proper plant development, including hypocotyls and leaf formation , stomatal opening (Wang et al, 2001), abscisic acid (ABA) responsiveness/germination (Colucci et al, 2002;Ullah et al, 2002;Lapik and Kaufman, 2003), and both ABA and BL regulation of gene expression (K.M. Warpeha, unpublished data).…”

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confidence: 99%

G-Protein-Coupled Receptor 1, G-Protein Gα-Subunit 1, and Prephenate Dehydratase 1 Are Required for Blue Light-Induced Production of Phenylalanine in Etiolated Arabidopsis

et al. 2006

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Different classes of plant hormones and different wavelengths of light act through specific signal transduction mechanisms to coordinate higher plant development. A specific prephenate dehydratase protein (PD1) was discovered to have a strong interaction with the sole canonical G-protein Gα-subunit (GPA1) in Arabidopsis (Arabidopsis thaliana). PD1 is a protein located in the cytosol, present in etiolated seedlings, with a specific role in blue light-mediated synthesis of phenylpyruvate and subsequently of phenylalanine (Phe). Insertion mutagenesis confirms that GPA1 and the sole canonical G-protein-coupled receptor (GCR1) in Arabidopsis also have a role in this blue light-mediated event. In vitro analyses indicate that the increase in PD1 activity is the direct and specific consequence of its interaction with activated GPA1. Because of their shared role in the light-mediated synthesis of phenylpyruvate and Phe, because they are iteratively interactive, and because activated GPA1 is directly responsible for the activation of PD1; GCR1, GPA1, and PD1 form all of or part of a signal transduction mechanism responsible for the light-mediated synthesis of phenylpyruvate, Phe, and those metabolites that derive from that Phe. Data are also presented to confirm that abscisic acid can act through the same pathway. An additional outcome of the work is the confirmation that phenylpyruvate acts as the intermediate in the synthesis of Phe in etiolated plants, as it commonly does in bacteria and fungi.

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GCR1 , the putative Arabidopsis G protein-coupled receptor gene is cell cycle-regulated, and its overexpression abolishes seed dormancy and shortens time to flowering

Cited by 97 publications

References 44 publications

GCR1 Can Act Independently of Heterotrimeric G-Protein in Response to Brassinosteroids and Gibberellins in Arabidopsis Seed Germination

GCR1 Can Act Independently of Heterotrimeric G-Protein in Response to Brassinosteroids and Gibberellins in Arabidopsis Seed Germination

Expression Profiling and Bioinformatic Analyses of a Novel Stress-Regulated Multispanning Transmembrane Protein Family from Cereals and Arabidopsis,

G-Protein-Coupled Receptor 1, G-Protein Gα-Subunit 1, and Prephenate Dehydratase 1 Are Required for Blue Light-Induced Production of Phenylalanine in Etiolated Arabidopsis

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