<i>GhMYB30</i>‐<i>GhMUR3</i> affects fiber elongation and secondary wall thickening in cotton

Wu, Aimin; Lian, Boying; Hao, Pengbo; Fu, Xiaokang; Zhang, Meng; Lu, Jianhua; Ma, Liang; Yu, Shuxun; Wei, Hengling; Wang, Hantao

doi:10.1111/tpj.16523

Cited by 4 publications

(1 citation statement)

References 69 publications

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“…It was identified as a crucial regulator of GhMUR3, responsible for encoding a xyloglucan galactosyltransferase essential for xyloglucan synthesis during fiber elongation; GhMYB30 was also found to directly bind to the GhMUR3 promoter, activating GhMUR3 expression (Figure 2). Additionally, GhMYB46 was confirmed as a target gene of GhMYB30 through EMSA, with GhMYB46 exhibiting a significant increase in GhMYB30-silenced lines, indicating the inhibitory role of GhMYB30 on GhMYB46 expression during fiber development [46] (Figure 2).…”

Section: Myb Transcription Factorsmentioning

confidence: 95%

Genetic and Molecular Regulation of Cotton Fiber Initiation and Elongation

Bai,

Scheffler

2024

Agronomy

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Cotton fiber, a crucial and sustainable resource for global textile production, undergoes a complex five-stage developmental process, encompassing initiation, elongation, transition, secondary cell wall biosynthesis, and maturation. These elongated single-cell fibers originate from the outer ovule epidermis. The development of cotton fibers involves intricate changes in gene expression and physiological processes, resulting in a nearly pure cellulose product that is vital for the global cotton industry. Decoding the genes associated with fiber development enhances our understanding of cotton fiber mechanisms and facilitates the cultivation of varieties with enhanced quality. In recent decades, advanced omics approaches, including genomics, transcriptomics, and proteomics, have played a pivotal role in identifying the genes and gene products linked to cotton fiber development, including the MYB transcription factor family, which coordinates cotton fiber development. Molecular studies have revealed the transcription factors, like MYB, WRKY, Homeodomain Leucine Zipper (HD-ZIP), and basic helix–loop–helix (bHLH), influencing fiber initiation and elongation. The intricate interplay of phytohormones, like auxin, gibberellic acid (GA), brassinosteroids (BRs), jasmonic acid (JA), ethylene, abscisic acid (ABA), and cytokinin, is explored, providing a comprehensive perspective on the shaping of cotton fibers. Numerous candidate genes and cellular processes affecting various aspects of fiber development hold promise for genetic engineering or marker-assisted breeding to improve fiber quality. This review presents a comprehensive overview of key achievements in cotton molecular biology, with a specific emphasis on recent advancements in understanding the transcription factors and phytohormones involved in cotton fiber initiation and elongation.

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Section: Myb Transcription Factorsmentioning

confidence: 95%

Genetic and Molecular Regulation of Cotton Fiber Initiation and Elongation

Bai,

Scheffler

2024

Agronomy

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Dynamics of duplicated gene regulatory networks governing cotton fiber development following polyploidy

Xiong,

Zhu,

Grover

et al. 2024

Preprint

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Cotton fiber development entails complex genome-wide gene regulatory networks (GRN) that remain mostly unexplored. Here we present integrative analyses of fiber GRNs using public RNA-seq datasets, integrated with multi-omics genomic, transcriptomic, and cistromic data. We detail the fiber coexpression dynamics and regulatory connections, validating findings with external datasets and transcription factor (TF) binding site data. We elucidate previously uncharacterized TFs that regulate genes involved in fiber-related functions and cellulose synthesis, and identify the regulatory role of two homoeologous G2-like transcription factors on fiber length. Analysis of duplicated gene expression and network relationships in allopolyploid cotton, which has two co-resident genomes (A, D), revealed novel aspects of asymmetric subgenomic developmental contributions. Whereas D-based homoeolog pairs drive higher overall gene expression from the D subgenome, TFs from the A subgenome play a preferential regulatory role in the fiber gene regulatory network. Following allopolyploid formation, it appears that the trans-regulatory roles of TFs diversified more rapidly between homoeologs than did the cis-regulatory elements of their target genes. Our approach underscores the utility of network analysis for detection of master regulators and provides fresh perspectives on fiber development and polyploid functional genomics, through the lens of co-expression and GRN dynamics.

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Daily glycome and transcriptome profiling reveals polysaccharide structures and correlated glycosyltransferases critical for cotton fiber growth

Swaminathan,

Grover,

Mugisha

et al. 2024

The Plant Journal

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SUMMARYCotton fiber is the most valuable naturally available material for the textile industry and the fiber length and strength are key determinants of its quality. Dynamic changes in the pectin, xyloglucan, xylan, and cellulose polysaccharide epitope content during fiber growth contribute to complex remodeling of fiber cell wall (CW) and quality. Detailed knowledge about polysaccharide compositional and structural alteration in the fiber during fiber elongation and strengthening is important to understand the molecular dynamics of fiber development and improve its quality. Here, large‐scale glycome profiling coupled with fiber phenotype and transcriptome profiling was conducted on fiber collected daily covering the most critical window of fiber development. The profiling studies with high temporal resolution allowed us to identify specific polysaccharide epitopes associated with distinct fiber phenotypes that might contribute to fiber quality. This study revealed the critical role of highly branched RG‐I pectin epitopes such as β‐1,4‐linked‐galactans, β‐1,6‐linked‐galactans, and arabinogalactans, in addition to earlier reported homogalacturonans and xyloglucans in the formation of cotton fiber middle lamella and contributing to fiber plasticity and elongation. We also propose the essential role of heteroxylans (Xyl‐MeGlcA and Xyl‐3Ar), as a guiding factor for secondary CW cellulose microfibril arrangement, thus contributing to fiber strength. Correlation analysis of profiles of polysaccharide epitopes from glycome data and expression profiles of glycosyltransferase‐encoding genes from transcriptome data identified several key putative glycosyltransferases that are potentially involved in synthesizing the critical polysaccharide epitopes. The findings of this study provide a foundation to identify molecular factors that dictate important fiber traits.

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GhMYB30‐GhMUR3 affects fiber elongation and secondary wall thickening in cotton

Cited by 4 publications

References 69 publications

Genetic and Molecular Regulation of Cotton Fiber Initiation and Elongation

Genetic and Molecular Regulation of Cotton Fiber Initiation and Elongation

Dynamics of duplicated gene regulatory networks governing cotton fiber development following polyploidy

Daily glycome and transcriptome profiling reveals polysaccharide structures and correlated glycosyltransferases critical for cotton fiber growth

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