<i>Helicobacter</i>

On, Stephen L. W.; Lee, Adrian V.; O’Rourke, Jani; Dewhirst, Floyd E.; Paster, Bruce J.; Fox, James G.; Vandamme, Peter

doi:10.1002/9781118960608.gbm01073

Cited by 11 publications

(21 citation statements)

References 167 publications

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“…Although EHH are frequently detected in clinical samples by PCR (Fox, ; Mateos‐Muñoz et al, ; Fresia et al, ), only occasionally they are isolated using medium and atmospheric conditions designed for Campylobacter spp. According to the Bergey’s Manual, there is no ideal method for the isolation of EHH and the recovery of these species is often optimized by the simultaneous use of selective and nonselective procedures (On et al, ). However, no standard culture method has been described for the isolation of EHH species so far.…”

Section: Resultsmentioning

confidence: 99%

Comparison of media and growth conditions for culturing enterohepaticHelicobacterspecies

Ochoa

Martínez

Fernández

et al. 2019

Lett Appl Microbiol

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This research aims to compare the culturing conditions for enterohepatic Helicobacter, evaluating culture media, incubation atmosphere and susceptibility to antimicrobials used to generate selective conditions. Four common media for the closely related genus Campylobacter (Columbia, Bolton, Brucella and CCDA agar), as well as the need for hydrogen in the microaerobic incubation atmosphere, were evaluated. Serial dilutions of 13 strains belonging to six species (H. apodemus, H. bilis, H. canicola, H. canis, H. equorum and Helicobacter sp.) were inoculated in each media and incubated at 37°C for 48 to 96 h using CampyGen (OXOID) and gaseous exchange (including hydrogen) in parallel. Columbia or Brucella agars were the most appropriate for culturing EHH (P < 0·05). However, there was no significant difference between the atmospheres evaluated (P = 0·13). In addition, minimal inhibitory concentration for six antibiotics showed that all isolates were resistant to trimethoprim, whereas for the rest of the antibiotics (cephalothin, cefoperazone, cefsulodin, teicoplanin and vancomycin) the inhibition range was between 8 and 64 μg ml−1. Our findings suggest that Columbia or Brucella media, regardless of the use of hydrogen, can be used for the EHH isolation. In addition, the concentration of antibiotics included in commercial campylobacteria supplements is suitable for EHH species recovery. Significance and Impact of the Study Enterohepatic Helicobacter (EHH) infections have been associated with several diseases in humans such as acute gastroenteritis, inflammatory bowel disease and hepatobiliary diseases. Although they are frequently detected in clinical samples by molecular methods, only occasionally they are isolated using culture conditions described for the taxonomic related pathogen Campylobacter sp. This is because the optimal conditions for the isolation of EHH have not yet been described, which results in an underestimation of the prevalence and clinical importance of these emerging pathogens. Therefore, this study provides insight for culturing EHH species.

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Section: Resultsmentioning

confidence: 99%

Comparison of media and growth conditions for culturing enterohepaticHelicobacterspecies

Ochoa

Martínez

Fernández

et al. 2019

Lett Appl Microbiol

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“…The G+C mol% content of H. cinaedi genomic DNA was previously estimated to be 37-38 mol% by the Tm method. [29], and our genome sequence data indicated similar content of 38.3-38.7%. The G+C mol% contents of genomic DNA from the H.…”

Section: Resultsmentioning

confidence: 51%

“…H. cinaedi infection causes many kinds of symptoms including fever, bacteremia, meningitis, erysipelas, cellulitis, arthritis, abdominal pain, proctitis, gastroenteritis, and diarrhea [14,29]. This organism is also known as an emerging nosocomial infection and risk factor for atherosclerosis [16].…”

Section: In Silico Hybridization-datamentioning

confidence: 99%

“…H. cinaedi, which is now classified as an enterohepatic Helicobacter species, has also been isolated from human blood and intestinal tract [26,34]. The organism identified as H. cinaedi has also been isolated from a wide range of animals, from domestic pets to wild animals, including dogs, cats, hamsters, rats, foxes, and rhesus monkeys [7,29,35]. H. cinaedi isolates from human, dog, and hamster formed distinct ribotype pattern groups according to their host source [17].…”

Section: Introductionmentioning

confidence: 98%

“…Data taken from Ref[2,29] and our results. a Data from whole genome sequence of each type strain (H. bilis PAGU 599 T , H. canis PAGU 598 T , and H. hepaticus 604 T )…”

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confidence: 97%

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Proposal of Helicobacter canicola sp. nov., previously identified as Helicobacter cinaedi, isolated from canines

Kawamura

Tomida

Miyoshi‐Akiyama

et al. 2016

Systematic and Applied Microbiology

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Dietary niche breadth influences the effects of urbanization on the gut microbiota of sympatric rodents

Anders

Mychajliw

Moustafa

et al. 2022

Ecology and Evolution

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Cities are among the most extreme forms of anthropogenic ecosystem modification, and urbanization processes exert profound effects on animal populations through multiple ecological pathways. Increased access to human‐associated food items may alter species' foraging behavior and diet, in turn modifying the normal microbial community of the gastrointestinal tract (GIT), ultimately impacting their health. It is crucial we understand the role of dietary niche breadth and the resulting shift in the gut microbiota as urban animals navigate novel dietary resources. We combined stable isotope analysis of hair and microbiome analysis of four gut regions across the GIT to investigate the effects of urbanization on the diet and gut microbiota of two sympatric species of rodents with different dietary niches: the omnivorous large Japanese field mouse (Apodemus speciosus) and the relatively more herbivorous gray red‐backed vole (Myodes rufocanus). Both species exhibited an expanded dietary niche width within the urban areas potentially attributable to novel anthropogenic foods and altered resource availability. We detected a dietary shift in which urban A. speciosus consumed more terrestrial animal protein and M. rufocanus more plant leaves and stems. Such changes in resource use may be associated with an altered gut microbial community structure. There was an increased abundance of the presumably probiotic Lactobacillus in the small intestine of urban A. speciosus and potentially pathogenic Helicobacter in the colon of M. rufocanus. Together, these results suggest that even taxonomically similar species may exhibit divergent responses to urbanization with consequences for the gut microbiota and broader ecological interactions.

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Helicobacter

Cited by 11 publications

References 167 publications

Comparison of media and growth conditions for culturing enterohepaticHelicobacterspecies

Comparison of media and growth conditions for culturing enterohepaticHelicobacterspecies

Proposal of Helicobacter canicola sp. nov., previously identified as Helicobacter cinaedi, isolated from canines

Dietary niche breadth influences the effects of urbanization on the gut microbiota of sympatric rodents

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