<i>Helicobacter pylori</i>Anti-CagA Antibodies: Prevalence in Symptomatic and Asymptomatic Subjects in Turkey

Abasıyanık, M. Fatih; Sander, Ersan; Salih, Barik A.

doi:10.1155/2002/589087

Cited by 20 publications

(19 citation statements)

References 29 publications

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“…Since the cytotoxin-associated gene product (CagA, 120 to 140 kDa) encoded by cagA is immunodominant, therefore, serum IgG antibodies to the CagA antigen may be a reliable marker of carriage of a CagA + H pylori strain [25] . In other studies the seroprevalence of anti-CagA antibody in H pylori-infected asymptomatic subjects was evaluated, so that it has been reported to be 56.7% at age 1-14 years in Saudia Arbia [17] , 82% at age 1.5-5 years in Bangladesh [18] , 46.9% at age 1-15 years in Mexico [26] , 88.5% and 81.3% at age 3-12 years in two counties of China [27] , 83% at age 20-65 years in Turkey [28] , 95.3% at age 20-60 years in Nigeria [29] . Accordingly, our observation confirms that CagA seroprevalence varies geographically.…”

Section: Discussionmentioning

confidence: 99%

Specific serum immunoglobulin G to H pylori and CagA in healthy children and adults (south-east of Iran)

Jafarzadeh¹,

Rezayati²,

Nemati³

2007

WJG

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AIM:To evaluate the serologic IgG response to H pylori and CagA across age groups and in healthy children and adults. METHODS:Totally, 386 children aged 1-15 years and 200 adults aged 20-60 years, were enrolled to study. The serum samples of participant were tested for presence of anti-H pylori and anti-CagA IgG by using ELISA method. RESULTS:The seroprevalence of H pylori in adults was significantly higher than that observed in children (67.5% vs 46.6%; P < 0.000003). In children, the seropositivity rate in males (51.9%) was significantly (P < 0.05) higher than that observed in females (41.7%). The prevalence of serum anti-CagA antibody was 72.8% and 67.4% in infected children and adults, respectively. The mean titer of serum anti-CagA antibodies was significantly higher among children in comparison to adults (64.1 Uarb/mL vs 30.7; P < 0.03). In infected children and adults the prevalence of serum anti-CagA antibody was higher in males compared to females (78.4% vs 66.3%; P = 0.07 and 75.6% vs 54.71%; P < 0.04, respectively). The age-specific prevalence of anti-H pylori and anti-CagA antibody (in infected subjects) was 37.6% and 59.57% at age 1-5 years, 46.9% and 75% at age 6-10 years, 54.9% and 79.45% at age 11-15, 59.01% and 83.33% at age 20-30 years, 66.6% and 60.52% at age 31-40 years, 73.46% and 63.88% at age 41-50 years and 75.75% and 60% at age 51-60 years with mean titer of anti-CagA antibody of 75.94, 63.32, 57.11, 52.06, 23.62, 21.52 and 21.80 Uarb/mL, respectively. There was significant difference between mean serum anti-CagA antibody in age subgroups (P < 0.001). CONCLUSION:These results showed that anti-H pylori and anti-CagA antibodies were common in the children and adults. The H pylori -specific antibodies influenced by age and sex of subjects. Moreover, it seems that males are more susceptible to infection with CagA + strains compared to females. The seroprevalence of anti-CagA antibody was increased with age, up to 30 years and then decreased. It was also found that the magnitude of the IgG response to CagA decreased with advanced age.

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Section: Discussionmentioning

confidence: 99%

Specific serum immunoglobulin G to H pylori and CagA in healthy children and adults (south-east of Iran)

Jafarzadeh¹,

Rezayati²,

Nemati³

2007

WJG

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“…Several studies have implicated CagA in the development of duodenal ulcer and gastric adenocarcinoma in most western populations (Covacci et al, 1993;Blaser et al, 1995;Torres et al, 1998;Bach et al, 1999;Miehlke et al, 2000;Abasiyanik et al, 2002; Nomura et al, 2002a, b;Oliveira et al, 2003;Wu et al, 2003), whereas the majority of reports from east Asian countries (Miehlke et al, 1996; Pan et al, 1997; Shimoyama et al, 1997;Maeda et al, 1998;Zheng et al, 2000;Groves et al, 2002; Lai et al, 2002), as well as those from Estonia (Andreson et al, 2002) and Argentina (Catalano et al, 2001), showed a very high prevalence of cagA-positive H. pylori, irrespective of clinical manifestation. Furthermore, it was reported that a PCR primer set that amplified cagA from H. pylori isolated in one country failed to detect cagA in isolates from another country (Miehlke et al., 1996; Pan et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

cagA genotype and variants in Chinese Helicobacter pylori strains and relationship to gastroduodenal diseases

Zhou

Zhang

et al. 2004

Journal of Medical Microbiology

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Previous studies have implicated CagA [encoded by cytotoxin-associated gene A (cagA)] in Helicobacter pylori-associated gastroduodenal pathology and distinct subgenotypes of cagA may circulate in different pathological manifestations of cagA-positive H. pylori infection. To investigate cagA genotype and variants in Chinese H. pylori strains and explore their relationship with gastroduodenal diseases, the cagA status of 82 Chinese H. pylori strains was examined and variation in size of the 39 region of cagA in 71 of these strains was analysed by PCR. cagA was detected in 28 (100 %) of 28 strains from peptic ulcer patients, two (100 %) of two strains from gastric cancer patients, 32 (94 . 1 %) of 34 strains from chronic gastritis patients and 17 (94 . 4 %) of 18 strains from healthy volunteers. PCR products of the cagA 39 variable region were obtained from 71 (92 . 2 %) of 77 Chinese H. pylori strains and could be classified into subgenotypes I, II and III, which gave PCR products of around 825, 900 and 950 bp, respectively. Subgenotype I cagA predominated in Chinese H. pylori strains (67/71), whereas subgenotype II cagA presented in two isolates from patients with chronic gastritis and subgenotype III presented in two isolates from healthy volunteers. Therefore, neither cagA nor its 39 region variants can be used as a sole marker for the presence of particular H. pylori-related gastroduodenal diseases in the Chinese population. INTRODUCTIONIt is generally accepted that Helicobacter pylori strains that produce CagA, an antigenic outer-membrane protein of variable molecular mass from 128 to 140 kDa, are highly virulent. Several studies have implicated CagA in the development of duodenal ulcer and gastric adenocarcinoma in most western populations (Covacci et al., 1993;Blaser et al., 1995;Torres et al., 1998;Bach et al., 1999;Miehlke et al., 2000;Abasiyanik et al., 2002; Nomura et al., 2002a, b;Oliveira et al., 2003;Wu et al., 2003), whereas the majority of reports from east Asian countries (Miehlke et al., 1996; Pan et al., 1997; Shimoyama et al., 1997;Maeda et al., 1998;Zheng et al., 2000;Groves et al., 2002; Lai et al., 2002), as well as those from Estonia (Andreson et al., 2002) and Argentina (Catalano et al., 2001), showed a very high prevalence of cagA-positive H. pylori, irrespective of clinical manifestation. Furthermore, it was reported that a PCR primer set that amplified cagA from H. pylori isolated in one country failed to detect cagA in isolates from another country (Miehlke et al., 1996; Pan et al., 1997). These discrepancies have led to the hypotheses that there may be several distinct forms of CagA with an uneven geographical distribution, that differences in cagA subgenotypes may provide a marker for differences in virulence among cagA-positive H. pylori strains and that only some forms of CagA are associated with severe gastroduodenal diseases.cagA is noted for its sequence diversity, both within and outside the variable 39 region of the molecule. Repeat sequences in the variable region are the m...

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“…Comparison between serology and the combination of the rapid urease test: Campylobacter-like organism test (CLO), histology and culture showed up to 97.8% sensitivity and 100% specificity (Abasiyanik et al, 2002).…”

Section: Resultsmentioning

confidence: 99%

Screening for Helicobacter pylori Infection among Asymptomatic University Students in Alexandria, Egypt, Using Non Invasive Laboratory Techniques

dir¹,

Kady²

2018

Int.J.Curr.Microbiol.App.Sci

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Helicobacter pyloriAnti-CagA Antibodies: Prevalence in Symptomatic and Asymptomatic Subjects in Turkey

Abstract: Antibodies to CagA antigen were prevalent in both groups, regardless of the presence of gastroduodenal disease.

Cited by 20 publications

References 29 publications

Specific serum immunoglobulin G to H pylori and CagA in healthy children and adults (south-east of Iran)

Specific serum immunoglobulin G to H pylori and CagA in healthy children and adults (south-east of Iran)

cagA genotype and variants in Chinese Helicobacter pylori strains and relationship to gastroduodenal diseases

Screening for Helicobacter pylori Infection among Asymptomatic University Students in Alexandria, Egypt, Using Non Invasive Laboratory Techniques

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