Specific serum immunoglobulin G to H pylori and CagA in healthy children and adults (south-east of Iran)

Abstract

AIM:To evaluate the serologic IgG response to H pylori and CagA across age groups and in healthy children and adults. METHODS:Totally, 386 children aged 1-15 years and 200 adults aged 20-60 years, were enrolled to study. The serum samples of participant were tested for presence of anti-H pylori and anti-CagA IgG by using ELISA method. RESULTS:The seroprevalence of H pylori in adults was significantly higher than that observed in children (67.5% vs 46.6%; P < 0.000003). In children, the seropositivity rate in males (51.9%) was significantly (P < 0.05) higher than that observed in females (41.7%). The prevalence of serum anti-CagA antibody was 72.8% and 67.4% in infected children and adults, respectively. The mean titer of serum anti-CagA antibodies was significantly higher among children in comparison to adults (64.1 Uarb/mL vs 30.7; P < 0.03). In infected children and adults the prevalence of serum anti-CagA antibody was higher in males compared to females (78.4% vs 66.3%; P = 0.07 and 75.6% vs 54.71%; P < 0.04, respectively). The age-specific prevalence of anti-H pylori and anti-CagA antibody (in infected subjects) was 37.6% and 59.57% at age 1-5 years, 46.9% and 75% at age 6-10 years, 54.9% and 79.45% at age 11-15, 59.01% and 83.33% at age 20-30 years, 66.6% and 60.52% at age 31-40 years, 73.46% and 63.88% at age 41-50 years and 75.75% and 60% at age 51-60 years with mean titer of anti-CagA antibody of 75.94, 63.32, 57.11, 52.06, 23.62, 21.52 and 21.80 Uarb/mL, respectively. There was significant difference between mean serum anti-CagA antibody in age subgroups (P < 0.001). CONCLUSION:These results showed that anti-H pylori and anti-CagA antibodies were common in the children and adults. The H pylori -specific antibodies influenced by age and sex of subjects. Moreover, it seems that males are more susceptible to infection with CagA + strains compared to females. The seroprevalence of anti-CagA antibody was increased with age, up to 30 years and then decreased. It was also found that the magnitude of the IgG response to CagA decreased with advanced age.

Serological Diagnosis of Helicobacter pylori Infection in Patients With a Polycystic Ovary Syndrome

“…In this study, the positive IgG at female cases with PCOS was 40% and in control group was 22%,which showed a significant difference (2). In a study of Jafarzadeh et al (17), 386 children aged 1 -15 years and 200 adults aged 20 -60 years were investigated in specific serum immunoglobulin G to H. pylori and CagA. They were reported the seroprevalence of IgG antibody against H. pylori in adults that was significantly higher than that observed in children (67.5% vs 46.6%; P < 0.000003).…”

“…Moreover, they indicated the prevalence of serum anti-CagA antibody that was 72.8% in infected children and 67.4% in adults. In addition, they were claimed that the mean titer of serum anti-CagA antibodies was significantly higher among children in comparison to the adult (64.1 Uarb/mL vs 30.7; P < 0.03) (17). In the present study, positive IgG antibody against H. pylori in women with PCOS was 79 (62%) and in women without PCOS was 76 (60-%).…”

Advantage of using a home-made elisa kit for detection of <i> Helicobacter pylori</i> infection over commercially imported kits

“…[5] The incidence of Hp infection in the developed countries ranges from 30 to 50% of the population [6] whereas that of developing countries such as Iran reaches 60-90% of the general population. [7][8][9] Since Hp infection, in addition to causing problematic symptoms, may lead to gastrointestinal ulcers and gastric malignancies, it is of utmost essence to detect the infection at early stages and pursue with eradication therapy and follow-ups.…”

The Antibody Response to Helicobacter pylori in the Sera from a Rural Population in the Central Anatolia Region of Turkey

“…In the studies from Korea and Iran, higher rates in males than females. 16,19) In two studies that were both performed via ELISA in Turkey, however, no differences were detected. 18,20) In our study, only half of the samples (53%) were positive for CagA antigen, a pathogenesis criterion for H. pylori.…”

“…This rate varies worldwide according to country, region, and age. The rate of anti-H. pylori IgG positivity for Iran has been reported as 53.75%; 16) for China, as 47% in 2003; 15) for Finland as 56% in 1973 and as 31% in 1994. 7) Helicobacter pylori positivity increases with age in all societies.…”