<i>Herbaspirillum</i>
            Species Bacteremia in a Pediatric Oncology Patient

Ziga, Edward; Druley, Todd E.; Burnham, Carey‐Ann D.

doi:10.1128/jcm.01479-10

Cited by 35 publications

(43 citation statements)

References 7 publications

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“…However, K. gyiorum is not present in the Vitek MS database. The identification of K. gyiorum by MALDI-TOF was confirmed by 16S rRNA gene sequencing using previously described methodology (2,3). The sequence was 100% identical to K. gyiorum using the NCBI nr/nt database; the next nearest matches were Bordetella spp.…”

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confidence: 99%

Two Cases of Kerstersia gyiorum Isolated from Sites of Chronic Infection

Pence¹,

Sharon

TeKippe³

et al. 2013

J Clin Microbiol

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c Kerstersia gyiorum is infrequently associated with human infection. We report the isolation of Kerstersia gyiorum from two patients: the first, a patient with chronic ear infections, and the second, a patient with a chronic leg wound. Both isolates were resistant to ciprofloxacin, which has not been previously reported. CASE REPORTSC ase 1. A 55-year-old man with a past medical history of chronic ear disease, alcoholism, and smoking (2 packs/day) was seen in the Barnes-Jewish Hospital otolaryngology clinic with a chief complaint of bilateral ear drainage. At the ages of 13 and 16, he had undergone canal wall-down mastoidectomies of the right and left ears, respectively. Since that time, he had reported some hearing loss and bilateral ear drainage. One month prior to his current encounter, the patient complained of increasing drainage from his left ear, which reportedly exhibited a reddish hue and an odor of "dead fish." At that time, the patient was prescribed 0.3% ciprofloxacin-0.1% dexamethasone otic solution (four drops, twice daily). At a follow-up visit 1 month later, he admitted to being only partially compliant with his prescribed regimen. During the same visit, the left mastoid cavity was suctioned and cleaned and a specimen was taken from the posterior pocket at the sinodural angle and submitted for aerobic bacterial culture. The patient was instructed to continue using ciprofloxacindexamethasone drops and expressed that he would make an effort to be more compliant.The direct Gram stain of the specimen submitted from the mastoid cavity showed no polymorphonuclear cells, moderate numbers of Gram-positive bacilli, and moderate numbers of Gram-negative bacilli. The culture grew abundant amounts of Corynebacterium amycolatum, as well as an abundant amount of a Gram-negative coccobacillus, which appeared in singles, pairs, and short chains on Gram stain (Fig. 1A). The isolate formed flat, opaque, gray colonies with spreading edges on blood (Fig. 1B) and chocolate agar, with a colony morphology somewhat resembling that of Alcaligenes spp. but lacking the characteristic "fruity" odor associated with this genus. On MacConkey agar, the isolate was non-lactose fermenting, but colonies had a slight lavender hue (Fig. 1C), which was especially evident when the colonies were picked up using a swab (Fig. 1D). The isolate was oxidase negative, spot indole negative, catalase positive, and nonmotile. An oxidation/fermentation (OF) glucose test was performed; the isolate was found to be a nonutilizer of glucose. Disks containing vancomycin and penicillin were added to subculture plates to obtain additional information about the isolate; there was no inhibition around the vancomycin disk, and a zone size of 16 mm was measured around the penicillin disk. A Vitek 2 Gram-negative identification (GNI) card (bioMérieux, Durham, NC) resulted in no identification. A RapID NF plus assay (Thermo Fisher Scientific, Lenexa, KS) was performed and gave a biocode of 010200, which resulted in an identification of Pseudomonas oryzihabi...

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confidence: 99%

Two Cases of Kerstersia gyiorum Isolated from Sites of Chronic Infection

Pence¹,

Sharon

TeKippe³

et al. 2013

J Clin Microbiol

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“…Herbaspirillum spp. have been recovered from the blood of patients with cystic fibrosis (22), leukemia (23,24), and cellulitis and bacteremia (25) and from sputum (22). They have been also found in arterial walls of aortic aneurysms (26).…”

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confidence: 99%

“…putei) and three distinct lineages were characterized (22,27). All these isolates have been misidentified as Ralstonia, Burkholderia cepacia complex, Cupriavidus pauculus, or Ochrobactrum anthropi (22,24) or could not be identified (22,23,25) by phenotypic identification methods used in the clinical laboratory.…”

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confidence: 99%

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Biochemical Characteristics, Adhesion, and Cytotoxicity of Environmental and Clinical Isolates of Herbaspirillum spp

et al. 2015

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e Herbaspirillum bacteria are best known as plant growth-promoting rhizobacteria but have also been recovered from clinical samples. Here, biochemical tests, matrix-assisted laser deionization-time of flight (MALDI-TOF) mass spectrometry, adherence, and cytotoxicity to eukaryotic cells were used to compare clinical and environmental isolates of Herbaspirillum spp. Discrete biochemical differences were observed between human and environmental strains. All strains adhered to HeLa cells at low densities, and cytotoxic effects were discrete, supporting the view that Herbaspirillum bacteria are opportunists with low virulence potential.

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“…Due to phylogenetic and phenotypic similarities, Herbaspirillum bacilli are often identified as BCC by automated microbial identification systems (4,5). The Vitek 2 microbial identification system with its expanded identification database (Advanced Expert System) is the automated system used by our Clinical Microbiology Laboratory to identify bacteria according to their biochemical profiles.…”

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confidence: 99%

Severe Community-Acquired Pneumonia with Bacteremia Caused by Herbaspirillum aquaticum or Herbaspirillum huttiense in an Immune-Competent Adult

et al. 2015

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cHerbaspirillum spp. are Gram-negative bacteria that inhabit soil and water. Infections caused by these organisms have been reported in immunocompromised hosts. We describe severe community-acquired pneumonia and bacteremia caused by Herbaspirillum aquaticum or H. huttiense in an immunocompetent adult male. CASE REPORT In early September 2014, a 46-year-old white male presented to a referring facility with fever, fatigue, and shortness of breath. His illness began 5 days prior to admission to our facility after he was drenched in rain during a fishing trip. He had a fever of 40°C, despite use of antipyretics, with chills, night sweats, anorexia, myalgia, and headache. Three days prior to admission, he had a transient period of dry cough for half a day that resolved spontaneously. The next day he developed sharp right-sided pleuritic chest pain, worsening dyspnea, and severe fatigue. When he presented to the referring facility, clinical findings were remarkable for a respiratory rate (RR) of 28/min with an oxygen saturation (SpO 2 ) level of 77% on room air which improved to 91% on 4 liters/min of oxygen via nasal cannula. His white blood cell (WBC) count was 7.7 ϫ 10 Ϫ3 /l with 55% bands, and bilateral alveolar infiltrates were noted on a chest X-ray. Analysis of his arterial blood gas revealed a pH level of 7.46, partial pressure of CO 2 (pCO 2 ) of 34 mm Hg, pO 2 of 53 mm Hg, SpO 2 of 89% on 4 liters/min of oxygen via nasal cannula, and HCO 3 of 24 meq/liter. Blood cultures were drawn, and he was treated with intravenous vancomycin, ceftriaxone, and azithromycin. His condition worsened and he was transferred to our university teaching hospital.The patient's past medical history was unremarkable, except for childhood asthma, atypical pneumonia as a teenager, and tonsillectomy. He was on no home medications. He lived on a farm in rural Missouri with his wife and had close contact with cattle and turkeys (he had birthed calves 6 months earlier and handled 15,000 baby turkeys 3 weeks prior to the onset of illness). A few weeks prior to admission, the patient returned from work with about 20 loosely attached ticks. He also cleaned a grain bin in his barn (contained mold and possible rat excreta) a week prior to presentation. He smoked cigarettes for 30 years and also consumed alcohol regularly. He denied sick contacts, animal bites or scratches, and recent travel.The results of a physical examination conducted on presentation to our facility were remarkable for temperature (38°C), heart rate (HR) (98 beats/min), blood pressure (BP) (141/88 mm Hg), and RR (30/min with use of accessory muscles). There was bilateral lower chest wall tenderness, coarse inspiratory crepitations were heard in left axilla and left lower chest, and diminished breath sounds were noted in the right axilla and right lower chest.The rest of the exam was unremarkable. Laboratory measurements on admission revealed a WBC count of 9.0 ϫ 10 Ϫ3 /l, granulocytes at 89.6% and no bands on automated differential determinations, hemoglobin at 13.4 g/dl, ...

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Herbaspirillum Species Bacteremia in a Pediatric Oncology Patient

Cited by 35 publications

References 7 publications

Two Cases of Kerstersia gyiorum Isolated from Sites of Chronic Infection

Two Cases of Kerstersia gyiorum Isolated from Sites of Chronic Infection

Biochemical Characteristics, Adhesion, and Cytotoxicity of Environmental and Clinical Isolates of Herbaspirillum spp

Severe Community-Acquired Pneumonia with Bacteremia Caused by Herbaspirillum aquaticum or Herbaspirillum huttiense in an Immune-Competent Adult

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